Clevelandella philipi, Kotyk & Bourland & Soviš & Méndez-Sánchez & Škaloud & Varadínová & Čepička, 2024

Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, Zoological Journal of the Linnean Society 202 (1), pp. 1-51 : 8

Clevelandella philipi sp.nov.

( Figs 6 , 7 ; Supporting Information, Tables S5, S6)

Description based on populations þom hosts ASS and STH þom Papua Neo Guinea: Small- to medium-sized Clevelandella * size in vivo 74–99 × 40–54 µm* usually about 90 × 49 µm; size in protargol preparations 59–88 × 35–47µm* usually about 75 × 41 µm in STH population; size in vivo 73–95 × 43–59 µm* usually about 87 × 49 µm; size in protargol preparations 49–78 × 29–41 µm* usually about 63 × 34 µm in ASS population. Body proper dorsoventrally flaưened; overall outline spade-shaped in ventral view* widest near junction of body proper with peristomial projection* cell outline asymmetric due to prominent less lobe* anterior end of cell tapers to blunt point; peristomial projection joins body proper near right margin; less cell margin conspicuously notched at base of peristomial projection* less lobe overhangs peristomial projection up to one-third of its length ( Figs 6A–D * 7B–D* G* H). Macronucleus usually inverted teardrop-shape ( Fig. 7B * E* F)* right margin slightly convex* or ellipsoidal ( Figs 6A * 7A* G* H)* karyophore unobserved. Chromatin coarsely granular. Micronucleus ellipsoidal (5.5 × 2.5 µm)* adjacent to right anterior margin of macronucleus ( Figs 6B * 7G). Swims slowly.

Somatic ciliature arranged in about 55 kineties. Cilia present only on anterior two-thirds of cell ( Fig. 7B ). About nine widelyspaced* curved* free transverse ciliary rows on peristomial projection. Right sutural kinetofragments numerous* well developed ( Fig. 7C * E). Peristomial projection averages about 24% of total body length in STH population* 28% in ASS population. Peristomial opening extends one-half ( Fig. 7A ) to three-quarters ( Fig. 7B * H) of length of peristomial projection. Adoral zone extends anteriorly about 51% (STH) to 57% (ASS) of body length to end near posterior margin of macronucleus* composed of an average of 30 (ASS) to 32 (STH) membranelles* adoral zone widest in peristomial projection* narrows anteriorly* POM as described for the family.

Occurrence: Clevelandella philipi was found in the hindgut of two Panesthiinae species from Papua New Guinea: ASS and STH from the same locality (Wanang 3). Of STH individuals* all of which were juveniles* 30% harboured C. philipi . Since only two adults of the STH population (both males) were dissected* no conclusions can be reached about possible life-stage specificity. In the ASS population* C. philipi was present in 37% of cockroach individuals regardless of age and sex. When present* the ciliate is typically abundant. In ASS the infection with C. philipi sp. nov. frequently (90%) co-occurred with C. ananiasi sp. nov. * requiring care in identification.

Figures

Figure 6. Schematic drawings of Clevelandella philipi sp. nov. from life (A) and asser protargol impregnation (B–D). A* ventral view of a typical individual.B* ventral view showing the oral apparatus and nuclear structures. C* ventral infraciliature.D* dorsal infraciliature.AZM* adoral zone of membranelles; CPK* cilia of circumperistomial kineties; CV* contractile vacuole; HL* hyaline subcortical layer; Ma* macronucleus; Mi* micronucleus; POM* paroral membrane; POV* peristomial overture; SK* somatic kineties. Scale bars:20 µm.

Figure 7. Clevelandella philipi sp. nov.* in vivo (A* B) and asser protargol impregnation (C–I). From STH (A–G) and ASS (H* I). A* ventral view showing thick* hyaline cortical layer (between black arrowheads)* cilia of the circumperistomial kineties (black arrow) and adoral membranelles (white arrow). B* ventral view showing posterior extent of somatic ciliation (white arrowheads).Note variability of macronuclear shape (A* B). C* ventral infraciliature with detail (inset) showing right sutural kinetofragments (white arrowheads). D* dorsal view showing infraciliature* ciliated circumperistomial kineties (black arrow)* looping kineties of peristomial projection (white arrow)* and cytoproct (black arrowhead).E* ventral view showing less-hand spiralling ventral somatic kineties (black asterisk) and right sutural kinetofragments (black arrowhead).F* dorsal view showing straight somatic kineties (white asterisk)* disordered basal bodies on less protuberance (white arrow)* and paroral membrane (black arrowhead). G* dorsal view showing micronucleus (white arrowhead). H* dorsal view showing straight dorsal somatic kineties (asterisk)* proximal margin of the peristomial overture (white arrow)* and looping kineties of peristomial projection (black arrowhead). I* detail view showing diplostichomonad paroral membrane composed of shorter right file (black arrowhead) and longer less file (white arrowhead) of basal bodies. Ma* macronucleus. Scale bars:20 µm (A–H) 10 µm (I).