Barrientosiimonas humi EM1075

Han, Ji Yeon, Lim, Oung Bin, Chea, So-Yi, Lee, Hyosun, Lee, Ki-Eun, Cha, In-Tae & Kim, Won-Jae Chi and Dong-Uk, 2022, A report on 20 unrecorded bacterial species of Korea isolated from soil in 2021, Journal of Species Research 11 (4), pp. 310-320 : 314

Description of Barrientosiimonas humi EM1075

Cells are Gram-stain-positive and coccus-shaped. Cell size is 0.55-0.57 μm. Colonies are circular, smooth, entire, and white pigmented after 3 days of incubation at 28°C on R2A. Positive for reduction of β -glucosidase (esculin hydrolysis), protease (gelatin hydrolysis), D-glucose, D-mannitol, N -acetyl-D-glucosamine, D-maltose, gluconate, adipate, malate, and phenyl-acetate; but negative for nitrates to nitrite (NO 3 ->NO 2 -), reduction of nitrates to nitrogen, indole production, glucose acidification, arginine dihydrolase, urease, β -Galactosidase (PNPG), L-arabinose, D-mannose, caprate, and citrate. Utilizes D-mannitol, D-glucose, D-melibiose, propionate, valerate, 3-hydroxy-butyrate, 4-hydroxy-benzoate, N -acetyl-D-glucosamine, D-sucrose, D-maltose, suberate, malonate, acetate, lactate, L-alanine, glycogen, and 3-hy- droxy-benzoate; but does not utilize salicin, L-fucose, D-sorbitol, L-arabinose, caprate, citrate, L-histidine, 2-ke- togluconate, L-proline, L-rhamnose, D-ribose, inositol, itaconate, 5-ketogluconate, and L-serine. Enzymatic activity reaction was positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, naphtol-AS-BI-phosphohydrolase, α -galactosidase, α -gluco- sidase, β -glucosidase, and α -mannosidase; but negative for lipase (C14), valine arylamidase, cystine arylamidase, trypsin, α -chymotrypsin, acid phosphatase, β -galactosi- dase, β -glucuronidase, N -acetyl- β -glucosaminidase, and α -fucosidase. Strain EM1075 (= NIBRBAC000509128) was isolated from a forest soil sample, Seoul, Korea.