Pandoraea sputorum EM

Han, Ji Yeon, Lim, Oung Bin, Chea, So-Yi, Lee, Hyosun, Lee, Ki-Eun, Cha, In-Tae & Kim, Won-Jae Chi and Dong-Uk, 2022, A report on 20 unrecorded bacterial species of Korea isolated from soil in 2021, Journal of Species Research 11 (4), pp. 310-320 : 311

Description of Pandoraea sputorum EM 0341

Cells are Gram-stain-negative and rod-shaped. Cell size is 0.6-0.8 μm. Colonies are circular, smooth, entire, and white pigmented after 3 days of incubation at 28°C on R2A. Positive for gluconate, caprate, adipate, malate, citrate, and phenyl-acetate in API 20NE; but negative for reduction of nitrates to nitrite (NO 3 ->NO 2 -), reduction of nitrates to nitrogen, indole production, glucose acidification, arginine dihydrolase, urease, β -glucosidase (esculin hydrolysis), protease (gelatin hydrolysis), β -galactosidase (PNPG), D-glucose, L-arabinose, D-mannose, D-mannitol, N -acetyl-D-glucosamine, and D-maltose. Propionate, caprate, valerate, citrate, L-histidine, 3-hydroxy-butyrate, 4-hydroxy-benzoate, L-proline, acetate, L-alanine, 3-hy- droxy-benzoate, L-serine are utilized; but does not utilize D-mannitol, D-glucose, salicin, D-melibiose, L-fucose, D-sorbitol, L-arabinose, 2-ketogluconate, L-rhamnose, N -acetyl-D-glucosamine, D-ribose, inositol, D-sucrose, D-maltose, itaconate, suberate, malonate, lactate, 5-keto- gluconate, and glycogen. Enzymatic activity reaction was positive for alkaline phosphatase, esterase (C4), leucine arylamidase, acid phosphatase, naphtol-AS-BI-phosphohydrolase in API ZYM; but negative for esterase lipase (C8), lipase (C14), valine arylamidase, cystine arylamidase, trypsin, α -chymotrypsin, α -galactosidase, β -galac- tosidase, β -glucuronidase, α -glucosidase, β -glucosidase, N -acetyl- β -glucosaminidase, α -mannosidase, and α -fucosidase. Strain EM0341 (= NIBRBAC000509124) was isolated from a forest soil sample, Seoul, Korea.