Colletotrichum chinense Ning Jiang & C.B. Wang, 2022

Wang, Cheng-Bin, Jiang, Ning, Xue, Han, Piao, Chun-Gen & Li, Yong, 2022, Colletotrichum chinense sp. nov. from Yucca gloriosa and C. quercicola sp. nov. from Quercus variabilis in China, MycoKeys 93, pp. 1-21 : 1

Colletotrichum chinense Ning Jiang & C.B. Wang sp. nov.

Fig. 3

Etymology.

Referring to the country, where the species was first collected.

Description.

Sexual morph not observed. Asexual morph developed on PDA. Setae and chlamydospores not observed. Conidiomata acervular, abundant, pulvinate, 200-500 μm diam. Conidiophores smooth-walled, unbranched, septate, sometimes constricted at the septa, hyaline, up to 40 µm long. Conidiogenous cells 6.5-19.5 × 3-8 µm (x - = 12.7 ± 2.7 × 5.3 ± 1.3 µm, n = 20), subglobose to ampulliform, smooth-walled, hyaline. Conidia 9.5-25.5 × 3.5-8.5 µm (x - = 14.8 ± 1.8 × 6 ± 1 μm, n = 50), L/W ratio = 2-2.7, cylindrical, obtuse at the apex, smooth-walled, hyaline, contents granular. Appressoria not observed.

Culture characters.

Colonies on PDA, flat, with an entire margin, with sparse aerial mycelium, covered with orange conidial masses, reaching 23-25 mm diam in 7 days at 25 °C. Colonies on MEA, flat, with no aerial mycelium, covered with slimy conidial masses, reaching 15-20 diam in 7 days at 25 °C. Colonies on SNA flat, sparse white hyphae, with an entire margin, reaching 12-15 diam in 7 days at 25 °C.

Specimens examined.

China, Beijing City, isolated from leaf spot of Yucca gloriosa L., Cheng-Bin Wang , 15 August 2020 (holotype CAF800056; ex-type living culture: CFCC 57501); Ibid (living culture: CFCC 57502) .

Notes.

Colletotrichum beeveri of the Colletotrichum boninense species complex and C. tofieldiae of the spaethianum species complex have been reported from Yucca before the present study ( Liu et al. 2022). Colletotrichum chinense from the present study is similar to C. beeveri in the conidial shape, but differs in conidial size (9.5-25.5 × 3.5-8.5 µm in PDA vs. 12.5-15.5 × 5.5-6.5 µm in SNA) ( Damm et al. 2012). In addition, C. tofieldiae differs from C. chinense by the falcate conidia ( Damm et al. 2009). Based on phylogenetic analyses using multi-locus sequences (ITS, gapdh, chs-1, act and tub2), C. chinense formed a sister clade to C. agaves in the Colletotrichum agaves species complex. The sequence identities between C. chinense CFCC 57501 and C. agaves LC0947 (21/578 ITS, 6/94 gapdh, 6/232 chs-1, 19/240 act and 26/505 tub2), C. euphorbiae CBS 134725 (31/578 ITS, 8/94 gapdh, 7/232 chs-1, 35/240 act and 32/505 tub2), C. ledebouriae CBS 141284 (29/578 ITS, 30/240 act), C. neosansevieriae CBS 139918 (28/578 ITS, 6/94 gapdh, 28/240 act and 27/416 tub2) and C. sansevieriae MAFF 239721 (29/578 ITS, 5/94 gapdh, 9/232 chs-1, 31/240 act and 44/505 tub2). ( Nakamura et al. 2006; Crous et al. 2013, 2015, 2016b; Liu et al. 2022) The chs-1 sequence of C. neosansevieriae CBS 139918 and the gapdh, chs-1 and tub2 sequences of C. ledebouriae CBS 141284 were missing. Morphologically, the conidia size of C. chinense are shorter than other species (Table 2 ).

Figures

Figure 3. Colletotrichum chinense (CFCC 57501; ex-type) A colony on PDA B colony on MEA C colony on SNA D conidiomata formed in PDA E conidiophores from the host F, G conidiophores H, I conidia F-I from PDA. Scale bars: 200 µm (D); 50 µm (E); 20 µm (F, G); 10 µm (H, I).