Kockovaella quanzhouensis C. Y. Chai & F. L. Hui, 2024

Chai, Chun-Yue, Xi, Zhi-Wen, Niu, Qiu-Hong & Hui, Feng-Li, 2024, Three new species of the genus Kockovaella (Cuniculitremaceae, Tremellales) from the phylloplane in China, MycoKeys 110, pp. 237-253 : 237-253

Kockovaella quanzhouensis C. Y. Chai & F. L. Hui sp. nov.

Fig. 4

Etymology.

The specific epithet qingyuanensis refers to the geographic origin of the type strain, Qingyuan Mountain, Quanzhou, Fujian.

Type.

China • Fujian Prov.: Quanzhou City, Qingyuan Mountain , in the phylloplane of Ilex asprella , 12 Mar 2022, W. T. Hu & S. B. Chu, NYNU 224192 (holotype GDMCC 2.325 T preserved as a metabolically inactive state, culture ex-type PYCC 9950 ) .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is cream to pale yellow, butyrous, smooth and glistening, with an entire margin. After 7 days in YM broth at 20 ° C, cells are ovoid, 2.1–4.9 × 3.3–5.6 μm, single or pairs, and reproduced by polar budding and the formation of stalked conidia. The conidia are separated at the distal end of the stalks from parent cells. After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar. Ballistoconidia are symmetrical and apiculate, 3.7–4.2 × 7.9–8.0 μm. Glucose fermentation is absent. Glucose, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, cellobiose, L-sorbose (delayed and weak), L-rhamnose, D-xylose, L-arabinose, D-ribose, D-mannitol, D-glucitol, D-gluconate (delayed), D-glucosamine, N-acetyl-D-glucosamine, and D-glucuronate are assimilated as sole carbon sources. Inulin, methyl-α-D-glucoside, salicin, D-arabinose, 5 - keto-D-gluconate, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, myo-inositol, DL-lactate, succinate, citrate, 2 - keto-D-gluconate, and glucono- 1.5 - lactone are not assimilated. L-Lysine is assimilated as sole nitrogen sources. Nitrate, nitrite, ethylamine, and cadaverine are not assimilated. Maximum growth temperature is 30 ° C. Growth in vitamin-free medium is positive. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Fujian Prov.: Quanzhou City, Qingyuan Mountain , in the phylloplane of Myrica sp. , 12 Mar 2022, W. T. Hu & S. B. Chu, NYNU 22425 .

GenBank accession numbers.

holotype GDMCC 2.325 T ( ITS: OP 278691 , D 1 / D 2: OP 278690 , RPB 1: PP 755336); additional strains NYNU 22425 ( ITS: PP 752295, D 1 / D 2: PP 752294, RPB 1: PP 755335).

Note.

Physiologically, Kockovaella quanzhouensis sp. nov. differs from six closely related species, K. calophylli , K. cucphuongensis , K. litseae , K. iteae , K. schimae , and K. vietnamensis , in its inability to assimilate D-arabinose, galactitol, succinate, citrate and glucono- 1.5 - lactone (Table 3 ).

Figures

Figure 4. Morphological characteristics of Kockovaella quanzhouensis sp. nov. NYNU 224192 T A colony morphology on YM agar after growth for 7 d at 20 ° C B budding cells after growth for 7 d in YM broth at 20 ° C C stalked conidia on PDA after growth for 7 d at 20 ° C D ballistoconidia on CM agar after growth for 7 d at 20 ° C. Scale bars: 10 μm.