Thirteen new species of Agromyzidae (Diptera) from the United States, with new host and distribution records for 32 additional species Eiseman, Charles S. Lonsdale, Owen Linden, John Van Der Feldman, Tracy S. Palmer, Michael W. Zootaxa 2021 2021-02-17 4931 1 1 68 49Y6M Spencer Spencer [151,547,1339,1366] Insecta Agromyzidae Ophiomyia Animalia Diptera 15 16 Arthropoda species carolinensis  ( Fig. 68)   Material examined.   NORTH CAROLINA: Durham Co., Durham, Leigh Farm Park,  4.v.2018, em.  25.v.2018, T. S. Feldman, ex  Symphyotrichum patens, # CSE4558, CNC1144104( 1♀);  10.v.2018, em.  25.v.2018, T. S. Feldman, ex  Symphyotrichum patens, # CSE4563, CNC1144103(1♁).   Hosts. Asteraceae:  Symphyotrichum*  patens(Aiton) G.L.Nesom. Wehave seen similar leaf mines on  S. cordifolium(L.) G.L.Nesom,  S. georgianum(Alexander) G.L.Nesom,  S. laeve(L.) Á.L̂ve & D.L̂ve,  S. lateriflorum(L.) Á.L̂ve & D.L̂ve,  S. novae-angliae(L.) G.L.Nesom, and  S. novi-belgii(L.) G.L.Nesom.  Leaf mine.( Fig. 68) Mostly greenish and interparenchymal, with portions sometimes becoming discolored reddish or brownish; initially long, narrow, and linear, later becoming an elongate, poorly defined blotch running along both sides of the midrib. The linear portion is sometimes only intermittently visible on the upper surface.  Puparium.Whitish; formed within the leaf, with the anterior spiracles projecting through the lower epidermis. On  Symphyotrichum patens, each puparium was found at the tip of one of the basal leaf lobes, which in one instance was 6 cmaway from the apparent end of the mine.  Phenology and voltinism.Our North Carolinaspecimens were collected as larvae in early May, with adults emerging later that month. In New York, larvae are present in June and July ( Scheffer & Lonsdale 2018).   Distribution. USA: NC, NY ( Scheffer & Lonsdale 2018). We have seen similar leaf mines in GA, IA, MA, MD, NH, NJ, VT, and ON.   Comments.The only previous host record for this species is that of “possibly  Symphyotrichumsp.” from Scheffer & Lonsdale (2018); the host genus is now confirmed. 3069844317 2018-05-04 2018-05-25 2018-05-04 T Durham Co. Leigh Farm Park Durham 15 16 1 North Carolina 3069844350 [176,1302,1483,1510] 2018-05-10 2018-05-25 2018-05-10 T Feldman 15 16 CSE4558, CNC1144104 1 1 NORTH CAROLINA 3069844460 CSE, CNC 15 16 CSE4563, CNC1144103 1 NORTH CAROLINA