Distinctive Collembola Communities in the Mesovoid Shallow Substratum: Entomobryomorpha of the Sierra de Guadarrama National Park (Central Spain) Baquero, Enrique Jordana, Rafael Ortuño, Vicente M. Zoosystema 2021 2021-01-26 43 3 37 78 Baquero & Jordana Baquero & Jordana 2021 [867,1336,1713,1741] Entognatha Entomobryidae Lepidocyrtus Animalia Collembola 22 57 Arthropoda species labyrinthi sp. nov.  ( Figs 8C, D; 11; 12; 13; Table 5)  urn:lsid:zoobank.org:act: 2D7EB763-FBF6-4CE1-89E1-350695051356   TYPE MATERIAL. —  Holotype.  Spain• ♀; Segovia, Sierra de Guadarrama, Montes Carpetanos, Majada Aranjuez(Northwest); 30T 4190 45231;  2071 ma.s.l.;  17.XI.2015; Ortuño et al.leg.; pitfall SSD(since  2.VI.2015); MZNA SSD-8(slide 04).    Paratypes. Spain• 5 specimenson slide and 70 inethyl alcohol; SSD-6, slides 05 and 11; Ortuño et al.leg.; MZNA•  6 specimens; SSD-7, slide 09; same data; MZNA•  11 specimenson slide and 20 inethyl alcohol; SSD-9, slide 06; same data; MZNA• 1 specimen; SSD-29, slide 08; same data; MZNA•  10 specimensin ethyl alcohol; SSD-6; same data; MNHN.  FIG. 12. —  Lepidocyrtus labyrinthiBaquero & Jordana n. sp.,Th II to AbdIII dorsal chaetotaxy.Abbreviations:see Material and methods.Symbols:●, ciliated Mc; ○, mes; ⦰, pseudopores; ▣, bothriotricha. Scale bar: 0.05 mm. TYPE LOCALITY. — Spain, Segovia, Sierra de Guadarrama, Montes Carpetanos, Majada Aranjuez (Northwest); 30 T 4190 45231; 2071 ma.s.l.  ETYMOLOGY. — The specific epithet “ labyrinthus” (of the labyrinth), refers to the presence of this species in the underground crack network of the mesovoid shallow substratum.   ADDITIONAL MATERIAL. — Sierra de Guadarrama, Segovia; Ortuño et al.leg.; MZNA•  13 specimens; SSD-1(  0.5 mdepth), slides 06, 07; same data; MZNA•  9 specimens; SSD-1(  1 mdepth), slides 06, 09 and 10; same data; MZNA•  6 specimens; SSD-2(  0.5 mdepth), slides 08, 09; same data; MZNA•  2 specimenson slide and 23 inethyl alcohol; SSD-2(  1 mdepth), slide 16; same data; MZNA•  3 specimens; SSD-3(  1 mdepth), slide 01; same data; MZNA•  1 specimen; SSD-16, slide 12; same data; MZNA•  1 specimen; SSD-22, slide 06; Madrid; same data; MZNA•  1 specimen; SSD-12, slide 11; same data; MZNA•  3 specimens; SSD-13, slide 05; same data; MZNA•  1 specimen; SSD-15, slide 07; same data; MZNA•  12 specimenson slide and 30 inethyl alcohol; SSD-23, slide 04; same data; MZNA•  1 specimen; SSD-24, slide 02; same data; MZNA•  1 specimen; SSD-28, slide 04; same data; MZNA•  3 specimenson slide and 26 inethyl alcohol; SSD-30, slide 04; same data; MZNA.  DIAGNOSIS. — Body violet more or less pigmented, ocular spot black, and antennae and body violetblue, with darker pigment dorsally, especially on tergites Th II-Abd III and distal part of the head; antennae with distal area pigmented and Ant IV totally pigmented; some specimens with posterior Th III and Abd I paler ( Fig. 8C, D). Head Mc Pa 5present; A 0, A 2and A 3as Mc, and A 2aas mes; posterior labial row with M 1, M 2, R*, E, L 1and L 2ciliated Mc (R half to two thirds of M; sometimes M 1absent and usually asymmetric); Th II a little projected overhead, i.e., not pointed completely downward; Th II-III without Mc; Abd II with chaeta a 2ppresent, a 3forward from ‘as’ sensilla and only m 3as ciliated Mc; AbdIV with four median ciliated Mc (C 1, B 4-6), three non-fan-shaped ciliated mic behind anterior bothriotrichum and bothriothrichal complex mic D 1ppresent; claw with four internal teeth: two basal and two unpaired; empodium acuminate; manubrial plate with three internal and 5-8 external chaetae.  DESCRIPTION  Size and color Body length up to 2.00 mm, including head (mean 1.64 mm, n = 17 adults), excluding antennae ( holotype: 1.80 mm). Color variable, from pale to dark violet almost whole body except last two abdominal segments and furcula; all specimens maintain transversal bands on Th II-Abd III; blue pigment on vertex of head and ocular patches Ant IV and tip of Ant II-III pigmented. Scales present on Ant I-II, ventral and dorsal head, thorax and abdomen dorsally, coxae I-III and femora-tibiotarsus I-III, dorsally and ventrally on manubrium and only dorsally on dens; manubrium and dens similar in length ( 0.37 mm, n = 15); non-annulated part of dens three times the length of mucro.  Head Antennal head ratio 1.58 (n =6). Ant IV without apical bulb, apical organite and accessory sensilla as in Figure 11B. Ant III sense organ with two curved and expanded sensilla, one of them bigger than the other ( Fig. 11C) three spiny guard sensilla, one of them blunt; on Ant II one distal similar but straight to Ant III expanded sensilla; Head Mc Pa5present, A 0, A 2and A 3as ciliated Mc, A 2aas mes; t, s and p chaetae present on ocular well (p as mes, bigger than the other), three scales in the area; head dorsal chaetotaxy ( Fig. 11A) with 5-8 antennal (An) ciliated Mc basomedian labial fields chaetae smooth. Four prelabral ciliated chaetae; labrum with three rows, ‘a’ row with four apically ciliated chaetae, ‘m’ and ‘p’ with five smooth chaetae ( Fig. 11D). Four labral papillae, conical or with a spinelike chaeta. Maxillary palp bifurcated with three smooth appendages ( Fig. 11E). Labial papilla (l.p.) E with finger-shaped process not reaching at base of apical appendage ( Fig. 11F). Labial row with M 1, M 2, R*, E, L 1and L 2ciliated Mc (R half to two-thirds of M; M 1sometimes absent and usually asymmetric) ( Fig. 11G). Postlabial chaetotaxy with 3+1 ciliated central Mc along the groove. 12+ 12 spinelike chaetae on posterior dorsal head.  FIG. 13. —  Lepidocyrtus labyrinthiBaquero & Jordana n. sp., Abd IV dorsal macrochaetotaxy, and detail of the shape of the accessory chaetae to bothriotricha. Abbreviations: see Material and methods. Symbols: ●, Mc; ○, mes; ⦰, pseudopores; ▣, bothriotricha; ▲, special chaetae. Scale bar: 0.05 mm.  Thorax chaetotaxy ( Fig. 12) Th II and Th III without Mc; Th II with ‘s’ and ‘ms’ in posterolateral position at level of m row; ThIII with two ‘a’ mic before psp, a 2, a 3, a 5, a 6, m 2(above psp), m 3, m 4, p 2-p 6, and on lateral tergite a mes with the lateral sensilla (sl) interiorly.  Abdomen chaetotaxy ( Figs 12; 13) Abd I with a 1before psp; m 1beside psp; a 3, a 6, m 3, m 4, m 6and p 5(with the ‘ms’ near a 3). Abd II, mi and ml chaetae present over bothriotrichum (m 2); a 2p(p) present as smooth mic; a 2(a) as smooth mic; m 3(B) present as ciliated Mc; ‘as’ over m 3and a 3upside over a 2(two times its length); m 3eand p 4(q 1and q 2) present as smooth mic; lm and ll present as pointed ciliated mic over bothriotrichum (a 5); a 6, m 4, m 6and p 5as smooth mic; m 5as Mc. Abd III, mi, ml and a 2as pointed ciliated mic over bothriotrichum (m 2); ‘as’ between a 2and m 3; m 3as smooth mic; a 3very up; p 3below m 3, and m 4as smooth mic; lm, li, ll and a 6as ciliated pointed mic surrounding bothriotrichum (a 5); im, em and am 6as small ciliated mic over m  5bothriotrichum; pm 6and p 6as ciliated Mc with d 3between them; ‘ms’ near p 5as smooth mic; p 8pas ciliated mes; a 7, a 8, m 7, m 8, p 7and p 8as smooth mic. AbdIV with four median mac (C 1, B 4-6; ratio between C 1- B 4/B 4-B 60.60-0.74, n = 3), and 6 lateral Mc (E 2-4, F 1-3); T 5as mic, D 3, T 6and T 7as mes (D 3as Mc in some specimens); before T 2bothriotrichum, usually, three pointed ciliated mic (a, m and D 1), with a supplementary ‘s’ chaeta present in only one specimen ( ♂) and asymmetric ( Fig. 13).  Legs Scales on legs (including all coxae). Trochanteral organ Vshaped with about 14-19 spine-like chaetae (n=3). Claw with four teeth on inner edge: basal pair at 50%, an unpaired median at 65%, and one minute unpaired subapical; two lateral teeth intermediate to base and paired, and one more basal dorsal tooth. Empodium acuminate, all with pe lamella serrated, other lamellae smooth (ae, ai, pi); claw:empodium ratio =1:0.8. Tibiotarsus III distally with one inner smooth chaeta similar in size to empodium; tenent hairs spatulated, smooth, similar in size to claw ( Fig. 11H).  Furcula Manubrium with scales dorsally and ventrally. Dens with scales only dorsally; manubrium and dens similar in length; manubrial plate (dorsally) with three internal ciliate Mc, between 5 and 8 (n =3) external chaetae, and 2 psp. Non-ringed area of dens three times the length of mucro ( 0,002 mm) ( Fig. 11I).  Macrochaetotaxy Reduced formula (from Gisin 1965, 1967a, b): R 0R 1R 2001/00/0101+3/0, paBq 1q 2, M1M2R*EL1L2 (* ½ to 2/3 of M).  ECOLOGY Species widely distributed in the three mountain ranges, found in the MSS of more than half of the sampling points ( Fig. 1A-C). Although it is present in the three bioclimatic zones, given the average catch and its frequent occurrence, it is more common with increasing altitude. Nevertheless, its greatest activity was recorded in SSD-6 of Canchal La Pedriza ( Fig. 3G, H), located in the oro Mediterranean forest zone, and accounts for 32% of the 234 Entomobryomorpha(not including  Orchesella) collected there ( Fig. 1E). At this site,  L. labyrinthiBaquero & Jordana n. sp.is syntopic with seven other species ( Figs 1F; 3H) of the group analyzed in this study.  REMARKS  Winkler (2016)and Mateos (2011)defined the  L. lignorumgroup as the species with the formula R 0R 1R 2001/00/0101+3 (with or without cephalic Mc S 0, also called Pa 5) and scales on antennae and legs, which currently includes the species:  L. barbulus Mateos 2011,  L. instratus Handschin, 1924,  L. juliae Mateos, 2011,  L. lignorum(Fabricius, 1775),  L. peisonisTraser & Christian, 1992,  L. ruberSchött, 1902,  L tellecheae Arbea & Jordana, 1990,  L. traseri Winkler, 2016,  L. uzeliRusek, 1985,  L. violaceus([ Geoffroy, 1762] Fourcroy, 1785). According to this definition, this new species belongs to this group. The species that share the traditional dorsal body macrochaetotaxy formula of Gisin (1965, 1967a, b) with this species include  L. barbulus,  L. instratus,  L. juliae,  L. lignorum,  L. peisonis,  L. traseri,  L. tellecheae,  L. uzeliand  L. violaceus.   Lepidocyrtus barbulusis differentiated by the labial formula; it also has a pale color.  Lepidocyrtus tellecheaehas scales in the antennal segments I-III, claw with three teeth and row ‘a’ of the labral series with pointed chaetae.  Lepidocyrtus juliae,  L. lignorumand  L. violaceushave labial papillae multispinate;  L. juliaealso has a characteristic coloration, with only four spots and  L. lignorumhas no pigment.  Lepidocyrtus peisonishas smooth labial papillae and row ‘a’ of labral chaetae pointed.  Lepidocyrtus instratushas three teeth on the claw and outer lamella of the empodium smooth.  Lepidocyrtus traserialso has the outer lamella of the empodium smooth, in addition labral chaetae of row ‘a’ are bifurcated.  Lepidocyrtus uzelihas the claw with only two teeth.  Lepidocyrtus juliae,  L. lignorum,  L. traseriand  L. violaceushave the row ‘a’ of labral chaetae bifurcated ( Table 5). The wide distribution of  L. labyrinthiBaquero & Jordana n. sp.does not correspond to the activity records, since it represents only 1% of the Entomobryomorphaand Entomobryidae, studied in this work, ( Figs 1D; 2A, B). 3027953462 2015-06-02 2015-11-17 2015-06-02 pitfall SSD MZNA Spain Majada Aranjuez 2071 Segovia Montes Carpetanos 22 57 SSD-8 1 1 holotype 3027953445 MZNA Spain Spain 22 57 SSD-6 5 paratype 3027953347 MZNA Spain 23 58 Spain 22 57 SSD-7 6 paratype 3027953376 MZNA Spain Spain 23 58 SSD-9 11 paratype 3027953373 MZNA Spain Spain 23 58 SSD-29 1 paratype 3027953393 MNHN Spain Spain 23 58 SSD-6 10 paratype 3027953377 MATERIAL, MZNA Ortuno Sierra de Guadarrama 23 58 1 3027953316 MZNA 5 Ortuno Sierra de Guadarrama 23 58 SSD-1 13 3027953367 [366,1099,215,2025] MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-1 9 3027953448 MZNA 5 Ortuno Sierra de Guadarrama 23 58 SSD-2 6 3027953457 MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-2 2 3027953383 [813,1437,294,318] MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-3 3 3027953444 [813,1311,321,345] MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-16 1 3027953425 MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-22 1 Madrid 3027953417 MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-12 1 Madrid 3027953348 MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-13 3 Madrid 3027953314 [961,1439,400,424] MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-15 1 Madrid 3027953410 MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-23 12 Madrid 3027953398 [960,1437,453,477] MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-24 1 Madrid 3027953380 [813,1287,479,504] MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-28 1 Madrid 3027953319 MZNA 1 Ortuno Sierra de Guadarrama 23 58 SSD-30 3 Madrid