First record of the genus Disholcaspis Dalla Torre & Kieffer (Hymenoptera: Cynipidae: Cynipini) in the Neotropics, with description of two new species from Panama Medianero, Enrique Nieves-Aldrey, José Luis Zootaxa 2011 2802 23 33 Medianero & Nieves-Aldrey Medianero & Nieves-Aldrey [151,803,1839,1865] Insecta Cynipidae Disholcaspis Animalia Hymenoptera 1 24 Arthropoda species bettyannae sp. nov.    Typematerial. HolotypeƤ ( Fig. 5A) (in Museo Nacional de Ciencias Naturales, Madrid, Spain( MNCN), cardmounted. Cat. nº 2108). PANAMA, Chiriquí, Volcan Baru 8º 47' 50 08” N, 82º 29' 35 9” W, 1800 m; ex gall on stems of  Quercus bumelioidesLiebm. (Fagaceae), gall collected 30.i.2008, insect emerged ii.08, E. Medianero leg. Paratypes: 3Ƥ same data as holotype, but collected 22.xii.2008, insect emerged i.09.Two paratypesin MNCN, one paratypein Maestría en Entomología, Universidad de Panamá( MEUP). Additionally, 1Ƥ paratypeof the typeseries was dissected for SEM observation (in MNCN). Etymology.Named after Dra. Betty Ann Rowe Catsambanis Vice-Rector Research/Graduate Programs, University of Panama.   Diagnosis and comments. The species is closely allied to  D. quercusvirensAshmeadfrom Florida, being similar in color and a majority of morphological characteristics. The species differ in the length of the notauli, the scutellar foveae, leg coloration and, mainly, in the gall that is induced.  Disholcaspis bettyannaehas the notauli distinct posteriorly and medially, indistinct anteriorly, smooth, broad and convergent posteriorly, whereas  D. quercusvirenshas notauli weakly impressed also posteriorly. The new species has scutellar foveae ellipsoidal, shallow but distinct, whereas the scutellar foveae are indistinct in  D. quercusvirens.  Disholcaspis bettyannaehas legs yellowish brown, whereas  D. quercusvirenshas legs light reddish-brown. The new species induces a small globular gall ( 6– 11 mmdiameter) that does not secrete nectar. Internally, it is of a compact, rather hard, corky texture and contains a free, oval yellowish larval cell ( Fig. 6C), whereas  D. quercusvirensinduces similar small, globular galls, internally spongy, which exude a sticky substance that attracts ants. In coloration,  Disholcaspis bettyannaealso resembles  D. unicolorKinseyfrom Mexicoand  D. mamillanaWeldfrom California ( USA) but differs from them mainly in the typeof gall that it induces and host plant. The galls of  D. unicolorand  D. mamillanaare large globular bullets ( 21 mmin diameter) with a nipple at the apex. Additionally, the anteroadmedian signa are not visible in  D. unicolor, whereas they are discernible in  D. bettyannae.A few more  Disholcaspisspecies described from Mexicoby Kinsey (Kinsey 1937)namely,  Disholcaspis laetaeKinsey,  D. purlansKinsey,  D. purpureaKinseyand  D. regina Kinsey 1937seems also closely similar to the Panamanian species here described. However the Mexican species differ from them in the general brightly cherry rufous coloration and the smaller size of adults, as well as for the smooth and shining gall surface and the different oak hosts, being associated mainly to dwarf oaks as  Q. laeta,  Q. reticulataand  Q. repanda.   Description.Body length 3.94 mm(range 3.64–4.19; N = 4) for females. Body uniformly amber and shiny with distal segment of antenna, anteroadmedian signa area, parapsidal signa, propodeal area (except by spiracular area), posteromedial area of metasoma, sternum, stipes, last segment of maxillary palp, labial palp and claws dark brown to black. Legs yellowish brown. Forewing hyaline with some very light infumation, veins dark brown to black.  Female.Head rugose, moderately pubescent with piliferous punctures, in dorsal view about 3.4 x wider than long. POL 1.5 x longer than OOL, posterior ocellus separated from inner orbit of eye by 2.2 x its longest diameter. Head in anterior view ( Fig. 1A) transversely ovate, 1.3 x wider than high, gena moderately broadened behind eyes, 0.26 x diameter of compound eye. Vertex, frons and occiput more rugose; face, gena with piliferous punctures, short delicate irradiating carinae from clypeus present, not reaching ventral margin of compound eye, absent medially above clypeus; head moderately pubescent, with relatively long golden setae, except vertex, frons with sparse, shorter setae. Clypeus more or less trapezoidal, 1.8 x wider than high, mostly smooth and moderately pubescent, ventral margin sinuate, slightly projecting over mandibles. Anterior tentorial pits visible; epistomal sulcus not indicated, clypeo-pleurostomal lines visible. Malar space 0.4 x height of compound eye, without malar sulcus. Toruli situated slightly above mid-height of compound eye; distance between antennal rim and compound eye 1.1 x width of antennal socket including rim. Ocellar plate not raised. Head, posterior view ( Fig. 1B) without occipital carina. Gula short; distance between occipital and oral foramina 1.5 x height of occipital foramen ( Fig. 1B). Hypostomal sulci well separate at oral fossa. Mouthparts ( Figs. 1A, 1B): mandibles strong, exposed, with dense setae in base, right mandible with three teeth, left with two teeth. Cardo of maxilla not visible, maxillary stipes relatively short, broad, about 2.2 x longer than wide. Maxillary palp five-segmented. Labial palp three-segmented. Antenna ( Fig. 1C) of moderate length, as long as 1/2 body length, with 13 flagellomeres; flagellum not broadening towards apex; with relatively long, erect setae, and elongate placodeal sensilla not well visible ( Fig. 1D). Relative lengths of antennal segments: 18:11:40:37:28:26:21:19:14:12:12:11:22. Pedicel sub-globose, small, 0.6 x as long as scape; F1-F6 long, slender, gradually decreasing in length. F1 1.08 x as long as F2. F7-F10 short and wide, F11 2.7 x longer than wide, 2.0 x as long as F10 ( Fig. 1D). Placodeal sensillae on F3-F11 disposed in one row of 8–10 sensillae in half dorsal area of each flagellomere. Mesosoma. Smooth to finely rugose, very densely pubescent with piliferous punctures, in lateral view 1.3 x as long as high, slightly convex dorsally. Pronotum, densely pubescent; lateral surface of pronotum with some longitudinal wrinkles dorsally; with long and dense setae ( Fig. 1F). Pronotum short medially, ratio of length of pronotum medially/laterally = 0.18. Pronotal plate indistinct dorsally ( Fig. 1E). Mesonotum ( Fig. 2A). Mesoscutum finely rugose, densely pubescent with piliferous punctures, slightly broader than long in dorsal view. Notauli distinct posteriorly, medially, faint anteriorly, smooth and convergent posteriorly, with an indistinct median mesoscutal impression. Anteroadmedian signa clearly visible. Parapsidal signa broad, smooth. Transscutal fissure narrow, clearly visible, deeply impressed, slightly sinuate. Scutellar foveae ellipsoidal, shallow, almost smooth, indistinctly separated medially, anterior and posterior margins relatively well marked, about 1/ 8 xas long as scutellum. Scutellum ( Fig. 2A) rounded from above, about 0.8 x length of mesoscutum, strongly reticulate-rugose, in lateral view extended posteriorly over dorsellum. Axillula densely pubescent, anterior, posterior margins marked. Mesopleuron smooth, densely pubescent with piliferous punctures ( Fig. 1F).   FIGURE 2.  Disholcaspis bettyannae: (A) Mesosoma dorsal view. (B) Propodeum. (C) Metatarsal claw. (D) Metasoma lateral view. (E) Detail of ventral spine of hypopygium, lateral view. (F) Detail of ventral spine of hypopygium. Metanotum ( Fig. 2B). Metapectal-propodeal complex. Metapleural sulcus reaching posterior margin of mesopectus at about mid-height of metapectal-propodeal complex ( Fig. 1F). Lateral propodeal carinae arched, poorly defined, with some secondary strong rugae laterally and dorsally on bare median propodeal area ( Fig. 2B), lateral propodeal area densely pubescent; nucha rugose. Legs. Densely pubescent; metatarsal claws with strong triangular basal lobe or teeth ( Fig. 2C). Forewing ( Fig. 5B) as long as body, radial cell 3.2 x longer than wide; open along anterior margin; areolet small, triangular, closed, distinct. R1 and M nearly straight, not reaching wing margin. R1 and Rs weakly pigmented. Rs slightly bowed. Rs+M not reaching basalis. 2r well pigmented, slightly projected medially. Apical margin of wing with short hair fringe. Metasoma ( Fig. 2D) large, as long as head and mesosoma combined, in lateral view as wide as high. Second metasomal tergite covering about 2/3 of metasoma, with patch of dense setae in its anteromedial area. Projecting part of hypopygial spine ( Fig. 2E) about 2.5 x as long as wide in ventral view ( Fig. 2F); laterally with long setae, longer than spine width, but not forming an apical patch.  Gall( Fig. 6A–C). Globose or bud-shaped, with broad base, monothalamic, densely pubescent with velvet or felt-like surface, growing singly or in clusters of two to six galls. Pale yellowish, sometimes tinged with pink or red when fresh ( Fig. 6A) and light brown when mature ( Fig. 6B). Internally with compact, rather hard, corky texture, containing free oval yellowish larval cell, ( Fig. 6C). Diameter of gall 6 to 11 mm, formed in stems of  Quercus bumelioidesLiebm., closely resembling that of  D. simulataKinsey, 1922,  D. canescensBassett, 1890and  D. quercussuccinipesAshmead, 1881known from USA, but, these galls are induced in different oak species.   Distribution.  Disholcaspis bettyannaewas found between 1431–2400 ma.s.l. at Chiriqui, Panama.   Biology.Only the asexual generation is known, inducing galls on  Quercus bumelioidesLiebm.(section Quercus). The galls are found between October and May during the dry season in Panama. The insects studied emerged in December and February.