Paraeutypella citricola = Eutypella citricola Paraeutypella guizhouensis gen. et sp. nov. and Diatrypella longiasca sp. nov. (Diatrypaceae) from China Dissanayake, Lakmali S. Wijayawardene, Nalin N. Dayarathne, Monika C. Samarakoon, Milan C. Dai, Dong-Qin Hyde, Kevin D. Kang, Ji-Chuan Biodiversity Data Journal 2021 2021-03-26 9 63864 63864 28D3A0DD-067B-5B36-9EF5-4D66BC3371CB L. S. Dissan., Wijayaw., J. C. Kang & K. D. Hyde L. S. Dissan., Wijayaw., J. C. Kang & K. D. Hyde 2021 Pyrenomycetes Diatrypaceae Paraeutypella GBIF Fungi Paraeutypella citricola Xylariales 0 63864 Ascomycota species citricola comb. nov.   Paraeutypella citricolaSpeg., in Anales del Museo Nacional de Buenos Aires 6: 245, (1898)  = Eutypella citricolaSyd. & P. Syd., Hedwigia 49: 80 (1909), nom. illegit., Art. 53.1  Materials   Type status: Holotype. Record Level:institutionID: LPS-2120  Type status:  Paratype. Occurrence:recordedBy: Nalin N. Wijayawardene; Taxon:kingdom: Fungi; phylum: Ascomycota; class: Sordariomycetes; order: Xylariales; family: Diatrypaceae; genus: Paraeutypella; specificEpithet: citricola; Location:country: China; county: Guiyang; locationAccordingTo: GuizhouUniversity Garden (South); Identification:identifiedBy:  L.S.Dissanayake; Event:year: 2019; month: October; day: 5; habitat: on a dead branch of Acer sp.; Record Level:type: paratype; institutionID: HMAS 290660, HMAS 290659; collectionID: culture KUMCC 20-0024, KUMCC 20-0023; institutionCode: Chinese Academy of Science, Kunmingand Chinese Academy of Science Herbarium; collectionCode: Kunming Institute of Botany Culture Collection  Description Saprobic on dead twigs of  Acer palmatum(Fig. 4). Sexual morph: Stromata immersed in bark of dead branches, erumpent, solitary or aggregated, circular to irregular in shape, superficial, carbonaceous. Ascomata 410-430 x430-470 μm(x̅ = 420 x450 µm, n = 10), perithecial, with groups of 4-6 perithecia arranged in a valsoid configuration, black, subglobose, clustered, immersed in ascostroma with ostiolar neck. Necks 360-390 μmlong (x̅ = 375 µm, n= 10), papillate, sulcate, central ostiolar canal filled with paraphyses. Peridium 27-40 μmwide, composed of two layers of textura angularis; inner layer cells hyaline, outer layer cells dark brown to black. Hamathecium composed of 3-7 μmwide (x̅ = 5 µm, n= 10), hyaline, paraphyses arising from base of perithecia, composed of long, narrow, unbranched, septate, guttulate, narrowing and apically truncate. Asci 70-75 x5-8 μm(x̅ = 72.5 x6.5 μm, n = 20), 8-spored, unitunicate, thin-walled, clavate to cylindrical clavate, long pedicellate (40-50 μm), J- apical ring. Ascospores 7-10 x2-3 μm(x̅ = 8.5 x2.5 μm, n = 30), overlapping biseriate, allantoid, hyaline to light brown, smooth, aseptate, usually with guttules. Asexual morph: Undetermined. Culture characteristics - Colonies on PDA, reaching 21 mm diam. after 2 weeks at 20-25oC, medium dense, circular to slightly irregular, slightly raised, cottony surface; colony from above: at first white, becoming buff; from below: yellowish-white at margin, yellow to brown at centre; mycelium yellowish.  Notes   Eutypella citricolawas described by Spegazzini (1898)from  Citrusin Argentina and has since been reported to cause dieback on various woody plants in warm temperate and tropical regions ( Farr and Rossman 2020).  Eutypella citricolastrains have previously been isolated from hosts such as  Citrus limon,  C. sinensis,  C. paradisi,  Salixspp.,  Schinus molle,  Ulmus proceraand  Vitis vinifera( Trouillas et al. 2011, Mehrabi et al. 2016). In our study, we provide additional information for  P. citricolafrom dead stems of  Acer( Sapindaceae) in China. In morphology, our collection (HMAS 290660) resembles  Eutypella, thus having pustulate stromata with stout, converging ostiolar necks and asci with eight spores. According to phylogenetic analysis, KUMCC 20-0024 closely groups with a collection of  E. citricola(IRAN 2349C), which was collected on dead branches of  Salixsp. ( Salicaceae) in Gilan Province, Iran ( Mehrabi et al. 2016) (Fig. 1). However, the IRAN 2349C strain is slightly different from our strain in having stromata with groups of 6-25 perithecia in a valsoid configuration and short ostiolar necks (100-300 µm), while our collection comprises stromata with groups of 4-6 perithecia in a valsoid configuration with a longer neck (356-385 μm). Based on phylogenetic analysis, both strains grouped in  Paraeutypellasensu stricto (Fig. 1). Hence, the name  Eutypella citricolais placed in  Paraeutypellaas  P. citricola.   Paraeutypella guizhouensis, the type of  Paraeutypella, morphologically resembles  P. citricolaboth having immersed stromata, perithecia each with a long ostiolar neck and allantoid, aseptate ascospores with an oil droplet at each end. However,  Paraeutypella citricoladiffers from  P. guizhouensisby the number of perithecia within a stroma (4-6 vs. 6-12). A comparison of the ITS 1.0% (6/576) and β-tubulin1.2% (5/406) between KUMCC 20-0024 and IRAN 2340C, ITS 1.0% (6/576) and β-tubulin1.0% (5/406) between KUMCC 20-0024 and HVGRF01, HVVIT07 has been made. 2021-01-01 2021-12-31 2021-01-01 0 63864 1 Holotype 2021-01-01 2021-12-31 2021-01-01 Nalin N. Wijayawardene, L. S. Dissanayake China Chinese Academy of Science L. S. Dissanayake Chinese Academy of Science Herbarium Kunming 0 63864 1 Guizhou Paratype 2021-01-01 2021-12-31 2021-01-01 Nalin N. Wijayawardene, L. S. Dissanayake China Chinese Academy of Science L. S. Dissanayake Chinese Academy of Science Herbarium Kunming 1 Guizhou Paratype