Updated description of Diospyros dussaudii Lecomte (Ebenaceae), with lectotypification and notes on its distribution Duangjai, Sutee Sinbumroong, Aroon Chalermwong, Porntawat Suekaew, Padungsak Khammongkol, Kwanjai Kiewbang, Wittawat Rueangruea, Sukid Thananthaisong, Theerawat Suddee, Somran PhytoKeys 2021 2021-11-04 184 67 82 6CX9D 2FE0C846-309B-522E-ADA7-0B7576548638 Lecomte, Notul. Syst. (Paris) 4: 113. 1928; in Fl. Indo-Chine 3: 954. 1930. Lecomte, Notul. Syst. (Paris) 4: 113. 1928; in Fl. Indo-Chine 3: 954. 1930. Magnoliopsida Ebenaceae Diospyros CoL Plantae Diospyros dussaudii Ericales 0 67 Tracheophyta species dussaudii   Type.  Laos. recule 4 October, 1913, Dussaud 120( lectotypedesignated here, P barcode P00721485; isolectotypes P barcode P00721486 and P 02141495). Figs 1and 3- 5.   Figure 3.  Diospyros dussaudii Ahabit Bbranch and leaves Ctrunk and bark C1slash of bark Dleaf venation, abaxial view E, Fmale inflorescences G, Hfemale flower I-Kimmature fruits L, Mmature fruits N, Ofruit in cross section showing immature seeds ( N) and mature seeds ( O). Photographs by Aroon Sinbumroong ( A-H, J, L-Mand O) and Sukid Rueangruea ( I, Kand N). Tree, 8-15(-20) m tall, up to 20 cmin diameter; trunk straight, without buttresses; outer bark grey, smooth and sparsely lenticellate; inner bark thick, reddish-brown or blackish-brown; sapwood white. Young branches covered with hairs, persistent or later glabrous. Buds covered by bifarious scales, ca. 3.0 cm long, outside densely pubescent. Leaves alternate; petiole 8-9 mmlong, with dense short brown hairs; blade subcoriaceous, oblong or elliptic-oblong, 12.0-16.2 x3.8-5.0 cm, base attenuate to cuneate, apex acute to acuminate; mid-rib prominent and finely pubescent above, glabrous or slightly pubescent or puberulous below; secondary veins 7-10 on each side, grooved above, raised below; glabrous adaxially, glabrous to tomentose abaxially. Male inflorescences fasciculate, axillary or on older branches below the leaves, covered with bifarious scales at base. Male flowers 4-merous; pedicel ca. 1 mmlong, pubescent; calyx tubular, with 4 short lobes, rounded at apex, 6.5-8.0 mm long, pubescent outside, glabrous inside; corolla white, salverform, pubescent outside, glabrous inside, tube ca. 8.0 mm long, cylindrical, lobes 4, lanceolate, 10.0 x3.0- 3.5 mm; stamens 12-16, arranged in 2 series, attached at base of corolla tube; filaments 1-3 mmlong, sparsely pubescent; anthers triangular, ca. 1.8 mmlong, apex apiculate, dehiscence sublateral. Female inflorescences 1-flowered, in the axils of leaves or on older branches below the leaves. Female flowers 4-merous; sessile or subsessile, pedicel up to ca. 3.0 mm long, bracteate; calyx green, tube ca. 6.0 mm long, pubescent outside, glabrous inside, lobes rounded, ca. 5.0 x3.0 mm, pubescent outside, glabrous inside; corolla creamy-white to pale yellow, urceolate, tube ca. 9.0 mm long, ca. 7.0 mm in diameter, lobes elliptic, ca. 10.0 x7.0 mm, pubescent outside, glabrous inside; staminodes 8, attached at the base of corolla tube; ovary globose, pubescent, 8-locular; style 1, ca. 1 mmlong, glabrous, stigmas 4. Fruits globose or depressed-globose, covered with dense orange hairs, 3.8-5.0 x4.0-5.0 cm, apex rounded, shortly apiculate, 8-locular; seeds 3-8 per fruit, light green when immature, turning yellow and orange when ripe; fruiting calyx divided to base, lobes ovate-oblong, spreading, ca. 4.0 x5.0 mm, pubescent outside, glabrous inside; fruit stalk ca. 5.0 mm long; mesocarp 3.0-4.0 mm thick, cream with brown dots. Seeds ellipsoid to planoconvex, two faces flat and one face convex, glabrous, ca. 4.0 x5.0 mm, black, endosperm smooth when young, but slightly ruminated when mature.   Figure 4.  Diospyros dussaudii Afruiting branchlet Baxillary bud Cmale inflorescence Dmale flower Eopened corolla of male flower, showing stamens Fstamens Gfemale flower, side view Hfemale flower, top view Iopened corolla of female flower, showing eight staminodes attached at the base of corolla tube Jovary and stigma of female flower Kfruit with four calyx Lcross section of 8-seeded fruit Mseeds, top view and side view and Nthe longitudinal section of seed, showing embryo. Drawn by W. Bhuchaisri from Sinbumroong & Suekaew 04042020-1( A, Band K-N), Sinbumroong 12092020-1( C-F) and Sinbumroong 08052020-1( G-J).Scale bars: 1 cm( G-J, M, N); 2 cm( A, B, K, L); 5 mm( D-F); 8 mm( C).  Additional specimens examined.   Laos. Khammouan: Nam Theun, Kaeng Luang, landing area near waterfall, 17°45'46"N, 105°20'21"E,  555 malt., 7 November 2005, fr.,  Newmanet al. LAO 833( E!, L!).  Thailand. Nakhon Phanom Province: Ban Phaeng District, Phu Langka National Park, trail behind park headquarters, 17°59'06.60"N, 104°07'58.20"E,  197 malt., 14 June 2013, fr.,  Suddeeet al. 4514( BKF); Ban Phaeng District, Phai Lom, Phu Langka National Park, Tat Pho Waterfall, 26 December 2019,  Khammongkol207( BKF).  Chumphon Province: Lamae District, 12 December 2014,  Sinbumroong12122014-1(fr.) ( BKF); ibid., 8 May 2020,  Sinbumroong08052020-1(female fl.) ( BKF); Tha Sae District, 10 December 2020,  Sinbumroong10122020-1(fr.) ( BKF).  Surat Thani Province: Tha Chana District, 4 April 2020,  Sinbumroong& Suekaew04042020-1(fr.) ( BKF); Ban Ta Khun District, Ratchaprapa Dam, 12 Sep. 2020,  Sinbumroong12092020-1(male fl.) ( BKF).  Distribution. Laos, Thailand (Fig. 5).  Ecology. Scattered along streams in tropical rain forests and dry evergreen forests. The species occurs in the understorey at altitudes of 100-300 m. The canopy of the tropical rainforest in Kaeng Krung National Park, Surat Thani, where the species occurs, is dominated by  Anisoptera costataKorth.,  Artocarpus rigidusBlume,  Dipterocarpus gracilisBlume,  Dipterocarpus kerriiKing,  Palaquium impressionerviumNg and  Parashorea stellataKurz.  Dacryodes rostrata(Blume) H.J. Lam forms a high sub-canopy with  Hopea oblongifoliaDyer,  Hydnocarpus castaneusHook. f. & Thomson,  Mesua ferreaL. and  Xerospermum noronhianum(Blume) Blume. Understorey species are  Barringtonia paucifloraKing,  Diospyros sumatranaMiq.,  Hydnocarpus nanusKing,  Koilodepas longifoliumHook. f. and  Microdesmis caseariifoliaPlanch. ex Hook.   Figure 5.Geographic distribution of  D. dussaudii. The solid star and star represent newly recorded localities in Thailand and the solid circle and circle represent localities in Laos. The map was created with SimpleMappr (http://www.simplemappr.net; Shorthouse 2010).  Conservation status. As the Laotian population remains unknown, we have classified the species as Data Deficient (DD) based on IUCN Red List Criteria ( IUCN 2019).  Phenology. Flowering May-October, fruiting May-April.  Note. When mature, specimens from peninsular Thailand exhibit hairy branches, whereas those collected from Phu Langka National Park have glabrous or glabrescent branches. The Laos specimens match those from Phu Langka National Park. As mentioned in Newman et al. (2007b), duplicates of Laotian specimens were deposited in three other herbaria in Laos, as well as at P. However, we were unable to study the specimens in Laos due to the COVID-19 pandemic. It is unsurprising that only a few specimens were collected from Laos because Laos has a low rate of botanical collection ( Middleton et al. 2019).  GenBank accession no. Sinbumroong 12122014-1: MZ457089 ( rbcL), MZ457093 ( atpB), MZ457101 ( matKand trnKintron), MZ457097 ( ndhF), MZ457105 ( trnLintron and trnL- trnFspacer) and MZ457109 ( trnS- trnGspacer). Sinbumroong & Suekaew 04042020-1: MZ457090 ( rbcL), MZ457094 ( atpB), MZ457102 ( matKand trnKintron), MZ457098 ( ndhF), MZ457106 ( trnLintron and trnL- trnFspacer) and MZ457110 ( trnS- trnGspacer). Sinbumroong 12092020-1: MZ457091 ( rbcL), MZ457095 ( atpB), MZ457103 ( matKand trnKintron), MZ457099 ( ndhF), MZ457107 ( trnLintron and trnL- trnFspacer) and MZ457111 ( trnS- trnGspacer). Khammongkol 207: MZ457092 ( rbcL), MZ457096 ( atpB), MZ457104 ( matKand trnKintron), MZ457100 ( ndhF), MZ457108 ( trnLintron and trnL- trnFspacer) and MZ457112 ( trnS- trnGspacer). 2005-11-07 Newman Laos 555 17.762777 Kaeng Luang 21 105.33917 Nam Theun 1 Khammouan 2013-06-14 2019-12-26 2013-06-14 Suddee, Khammongkol Thailand Ban Phaeng District 197 17.985167 Ban Phaeng District 1 104.132835 Phu Langka National Park 1 Nakhon Phanom Province 2014-12-12 2020-12-10 2014-12-12 Sinbumroong Thailand Ban Phaeng District 197 17.985167 Tha Sae District 1 104.132835 Lamae District 1 Chumphon Province 2020-04-04 2020-09-12 2020-04-04 Sinbumroong, Suekaew Thailand Tha Chana District Ratchaprapa Dam Ban Ta Khun District 1 Surat Thani Province