Rediscovery of Rubus pendulus Rusby (Rosaceae) and a new record for the flora of Ecuador and Peru Espinel-Ortiz, David A. Rodriguez, Carla J. Romoleroux, Katya PhytoKeys 2023 2023-06-02 227 109 122 DB2EC27D-7B1F-5F04-AC51-B265125BBCC2 Rusby, Torreya 33: 41. 1933. Rusby, Torreya 33: 41. 1933. Magnoliopsida Rosaceae Rubus CoL Plantae Rubus pendulus Rosales 0 109 Tracheophyta species pendulus   Figs 1 , 2 , 3   Type.    Colombia. Huila: Balsillas, at Balsillas river, edge of forest,  2000-2100 m, 03-05 Aug 1917,  H.H. Rusby& F.W. Pennell719( holotype: NY (NY-424649)).   Figure 1.  Rubus pendulusRusby Ainflorescence Bhabit and leaves Cbranch D5-foliolate leaf Eleaf adaxial surface Fleaf abaxial surface Gpedicel Hflower Isepal adaxial surface Jsepal abaxial surface Kfruit ( Kbased on Fernandezet al. 606 (QCNE) Bbased on Espinel-Ortiz et al. 301 (QCA) C-Fbased on Espinel-Ortiz et al. 304 (QCA), A, G-Jbased on Espinel-Ortiz et al. 382 (QCA)). Illustrations by Carla Rodriguez.  Description.  Woody vinegrowing up to 10 m long, or scandent or climbing shrub, with all prickles from the base 1⁄3-2⁄3 sparsely villous-hirsute with red setose hairs, glabrous towards the apex, eglandular or with subsessile glands. Branchesobtuse-angled, red to slightly brownish, with red setose hairs, and hirsute, 3.4-9.4 mm diam., eglandular or with some setose hairs ending in a gland, unarmed or with up to 5 prickles (per total area of 5 cm long of the branch), gradually narrowed from a broad base, curved at the apex, 1.1-4.1 x1.2-7.4 mm. Stipulesasymmetrically narrow, subulate, 4.7-9.7 x0.4-1.8 mm, margin entire, chartaceous; adaxial surface sparsely hirsute on veins, with red subsessile glands on margin; abaxial surface with red setose hairs on veins and towards the margin, and hirsute, with red sessile and subsessile glands. Petioles4.8-10.6 (-15.2) cm long, with red setose hairs, and hirsute, with 17-35 prickles, gradually narrowed from a broad base, curved at the apex, 0.9-2.8 x0.6-2.8 mm; basal petiolules 3.0-6.5 mm long, unarmed or with up to 5 prickles, gradually narrowed from a broad base, curved at the apex, 0.7-1.1 x0.3-1.1 mm; lateral petiolules 7.0-29.7 (-45.5) mm long, with 3-17 (-34) prickles, gradually narrowed from a broad base, curved at the apex, 0.6-2.0 x0.4-1.6 mm; terminal petiolules 2.8-5.1 (-9.0) cm long, with 8-27 (-42) prickles, gradually narrowed from a broad base, curved at the apex, 0.6-2.8 x0.4-3.1 mm. Leavestrifoliate to 5-foliate; leaflets ovate to elliptic, base subcordate or asymmetrically subcordate, apex acuminate, margin serrate or bidentate towards the apex, basal leaflets (2.6-) 5.3-7.7 x(1.1-) 2.5-4.0 cm, lateral leaflets 7.1-14.3 x3.7-7.0 cm, terminal leaflet (6.7-) 10.4-15.1 x(3.8-) 4.8-7.3 cm, chartaceous, with 10-19 secondary veins, adaxial surface bullate, sparsely villous-hirsute on each bubble, and densely villous hirsute on the midvein and secondary veins, with red subsessile glands, unarmed, abaxial surface glabrous with red setose hairs, and villous only on the veins, with red subsessile glands on the veins, rarely unarmed or with 8-18 (32) prickles on the primary vein, gradually narrowed from a broad base, curved at the apex, 0.2-1.8 x0.3-2.1 mm. Inflorescencescompact, compound, terminal and axillary cymes, 6-53-flowered, 8.2-15.3 cm long, with simple or trifoliate leaves below; peduncles terete, red to slightly brownish, (5.7-) 8.1-20.3 (-48.7) mm long, villous with abundant red setose hairs, eglandular, unarmed or with up to 14 prickles, gradually narrowed from a broad base, curved at the apex, 1.4-2.2 x0.4-1.6 mm; pedicels terete, red to slightly brownish, 2.4-7.5 (-9.1) mm long, villous with abundant red setose hairs, eglandular, with 6-20 prickles, gradually narrowed from a broad base or triangular, curved at the apex, 0.8-2.4 x0.1-1.4 mm. Flowers8.21-15.25 mm diam.; sepals 5, obovate to elliptic or slightly lanceolate, apex mucronulate, margin entire, 5.1-7.9 x2.5-4.6 mm, greenish-red to red, adaxial surface concave, villous-sericeous, and tomentose towards the apex and the margin, with sessile and subsessile glands, unarmed, abaxial surface convex, tomentose, with subsessile glandular, unarmed; petals 5, narrowly obovate to elliptic, margin entire, 9.0-13.9 x6.1-7.8 mm, fuchsia to pink, glabrous, eglandular, adaxial surface deeply concave, abaxial surface deeply convex, stamens with anthers glabrous, filaments pale pink, glabrous; pistils, stigmas glabrous, styles slightly hirtellous, ovaries densely villous. Fruitsgreen to red when immature, and black at maturity, ovoid to globose, 7.8-15.4 x6.6-11.0 mm (when dry); drupelets 66-115 per receptacle, 2.1-4.3 x1.1-2.8 mm (when dry), sparsely villous.   Figure 2.  Rubus pendulusRusby Ahabit Btrifoliate leaf adaxial surface Cbullate leaf adaxial surface Dflowers. Photos by David A. Espinel-Ortiz.  Specimens examined.   Colombia. - Huila: Neiva, Vereda La Plata, Finca La Colonia (Antigua Carolina), 2000 m, 31 Oct 1996(fl), F. Llanos & W.F. Gerardino 2797(COL). -  Magdalena: Sierra Nevada de Santa Marta, Finca Cecilia, Quebrada Indiana, ca 10°59.000'N, 73°58.000'W, ca 1750 m, 03 Sep 1972(fl), J.H. Kirkbride 2082(COL); Sierra Nevada de Santa Marta, Finca Los Arroyitos, ca 10°56.000'N, 73°58.000'W, ca 1800 m, 07 Oct 1972(fl, fr), J.H. Kirkbride 2436(COL, US (US-3733777)). -  Santander: Between Piedecuesta and Las Vegas, 2000-2500 m, 19-24 Dec 1926(fr), E.P. Killip & A.C. Smith 15567(NY); Municipio Onzaga, Vereda Chaguaca, 2640 m, 30 Mar 1976(fr), J.H. Torres, G. Lozano & S. Diaz539(COL). -  Cundinamarca: Facatativa, Alto de PenaNegra, 2810-2820 m, 29 May 1941(fl, fr), H. Garcia-Barriga& R. Jaramillo 104033(US (US-3733540)). -  Bogota-DC: 25 milesSW of  Bogota, 18 Mar 1952(fr), G.M. Darrow s.n.(US (US-3733541)). -  Cesar: Municipio Valledupar, Corregimiento de Puerto Bello, 1200-2000 m, 13 Jul 1983(fl), Cuadros H. V. 1685(COL).  Ecuador. - Pichincha: San Josede Mindo, Nono-Tandayapa road, route of the OCP Heavy Crude Oil Pipeline, Cerro Castillo and La Bola, 00°01.750'S, 78°40.984'W, 2600 m, 05 Oct 2001(fl, fr), D. Fernandez, E. Toapanta, M. Mites & C. Morales 606(MO, QCNE (QCNE-159936)); Quito, Nanegalito, viaa San Tadeo, AreaProtegida Privada Bellavista, 00°02.170'S, 78°42.067'W, 2297 m, 03 Dec 2021, D.A Espinel-Ortiz & H.G. Abad 300(QCA (QCA-244065, QCA-7010819 to QCA-7010822 and QCA-7010828)); same locality as for preceding, 00°02.178'N, 78°42.227'W, 2297 m, 03 Dec 2021, D.A Espinel-Ortiz & H.G. Abad 301(QCA (QCA-244068 and QCA-7010829 to QCA-7010831)); Quito, Nanegalito, viaal AreaProtegida Privada Bellavista desde carretera E26, 00°00.077'N, 78°41.356'W, 2281 m, 07 Dec 2021, D.A Espinel-Ortiz & H.G. Abad 303(QCA (QCA-244067 and QCA-7010825 to QCA-7010827)); same locality as for preceding, 00°02.178'S, 78°42.227'W, 2315 m, 20 Apr 2022, D.A Espinel-Ortiz & H.G. Abad 327(QCA); same locality as for preceding, 00°02.274'S, 78°42.275'W, 2303 m, 20 Apr 2022, D.A Espinel-Ortiz & H.G. Abad 328(QCA); same locality as for preceding, 00°02.281'S, 78°42.316'W, 2 m, 16 May 2022(fl), D.A Espinel-Ortiz & H.G. Abad 382(QCA); Quito, Nanegalito, El Golan, between El Aliand El Porvenir, 00°06.570'N, 78°35.150'W, 2444 m, 25 May 2021(fl), C.E. Ceron& C.I. Reyes-Tello 88459(QAP (QAP-106468 and QAP-106757)); Quito, Nanegalito, El Golan, between Eden Magicoand El Porvenir, 00°05.270'N, 78°33.230'W, 2402 m, 10 Jul 2021, C.E. Ceron, C.I. Reyes-Tello, D. Bacuilima & A. Acosta88667 (QAP (QAP-106886)); Quito, Yunguilla, pasando la entrada a la comunidad El Golan, 00°06.485'N, 78°33.207'W, 2641 m, 08 Dec 2021, D. Espinel-Ortiz & H.G. Abad304 (QCA (QCA-244066 and QCA-7010824). -  Napo: National Park Los Llanganates, Salcedo-Tena road, km 60, "La Poderosa''ranch, descending to Mulatos river, 4 km, 00°57.000'S, 78°14.000'W, 2500-2870 m, 16 Mar 1995(fl, fr), H. Vargas & D. Sandoval 451(MO (MO-1610744), NY). -  Loja: Ca. 5 kmof Paso de Sabanilla, on road Yangana-Valladolid, 04°27.00'S, 79°10.000'W, 2500 m, 03 Sep 1985, S. Laegaard55178(AAU). -  Morona Santiago: Sangay National Park, Guamote-Macas road, near Purshi-Zuna, 02°11.000'S, 78°20.000'W, 2400-2700 m, 07 Jun 1998, C.E. Ceron36281(QAP (QAP-91)). -  Zamora Chinchipe: Nanguipa Cordillera, Cerro Colorado, about 8 kmby air SSE of Nambija, 20 kmESE of Zamora, montane cloud forest, 04°07.483'S, 78°46.417'W, 2500 m, 18 Feb 2002(fr), D. Neill, W. Quizhpe, J. Manzanares, A. Hirtz, T. DeLinks & C. Cole 13778(MO, QCNE (QCNE-162651)); Parque Nacional Yacuri, San Andres, colecciones en la viaJimbura-Zumba, ca 500 mdel rioIsimanchi, 04°47.100'S, 79°22.668'W, 2653 m, 29 Apr 2015,  A.J. Perez, N. Zapata, W. Santillan& R. Jimenez8997(QCA (QCA-233885)).  Peru. - Cajamarca: Cutervo, San Andresde Cutervo, Parque Nacional Cutervo, arriba de Sucedal pasando por Chorro Blanco, 06°11.353'S, 78°41.578'W, 2250 m, 03 Aug 1988(fr), C. Diaz& H. Osores 2942(F, MO).   Figure 3.  Rubus pendulusRusby. Collection Fernandezet al. 606 (QCNE) with flowers and fruits.  Distribution.   Rubus pendulusis distributed in the Northern and Central Andes (Fig. 4). In Colombia, it is known from seven collections in BogotaDC and the Departments of Cesar, Cundinamarca, Huila, Magdalena and Santander. In Ecuador it was found in the provinces of Loja, Morona Santiago, Napo, Pichincha and Zamora Chinchipe. Lastly, from Peru it is known from one collection in Cajamarca. This species inhabits the Andean cordillera from 2000 to 2900 m a.s.l.; however, there were two specimens from Magdalena which showed a lower distribution from ca 1700 to 1800 m.   Figure 4.Distribution map of  Rubus pendulus(black circle) in Colombia, Ecuador and Peru.  Ecology. This species occurs in montane cloud forests dominated by trees and shrubs and in nearby disturbed areas.  Rubus penduluscan be found living in sympatry with  Rubus adenotrichosSchltdl.,  R. boliviensisFocke,  R. longistipularis,  R. porphyromallosand  R. urticifolius. Flowering and fruiting collections dated from February, March, May and October.  Conservation status.   Rubus pendulusis known from at least 18 localities, impacted by human activities, including regression to agriculture and road openings. Following the IUCN (2022)guidelines, based on the geographic distribution and altered land use at the localities, this species should be categorized as least concern (LC). 1917-08-03 1917-08-05 1917-08-03 H. H. Rusby, F. W. Pennell Colombia 2050 Balsillas river Balsillas 1 Huila holotype