Aeminium ludgeri J. Trovao , I. Tiago & A. Portugal

Trovao, Joao, Tiago, Igor, Soares, Fabiana, Paiva, Diana Sofia, Mesquita, Nuno, Coelho, Catarina, Catarino, Lidia, Gil, Francisco & Portugal, Antonio, 2019, Description of Aeminiaceae fam. nov., Aeminium gen. nov. and Aeminiumludgeri sp. nov. (Capnodiales), isolated from a biodeteriorated art-piece in the Old Cathedral of Coimbra, Portugal, MycoKeys 45, pp. 57-73 : 62-63

Aeminium ludgeri J. Trovao, I. Tiago & A. Portugal sp. nov. Figure 2and Suppl. material 5: Figure S4.

Type.

Portugal, Coimbra (40°12'32"N, 8°25'38"W), isolated from a biodeteriorated limestone art piece in the Old Cathedral of Coimbra, 22 November 2016, Igor Tiago, (holotype: permanently preserved in metabolically inactive state DSM 106916).

Etymology.

In memory of our late colleague Ludgero Avelar.

Diagnosis.

Phylogenetic analysis based on the concatenated ITS rDNA, LSU and rpb2 dataset considered in the present study clustered the retrieved strains in a monophyletic separate lineage related to the families Neodevriesiaceae and Extremaceae . Therefore, a new family Aeminiaceae fam. nov, a new genus Aeminium gen. nov., and a new species Aeminium ludgeri sp. nov. in the order Capnodiales are here proposed.

Description.

Mycelium initially consisting of branched, septate, smooth, subhyaline to pale green, 2-3 μm wide hyphae. Hyphae moniliform, gradually becoming widen, thick-walled, darker and developing into meristematic conidial chains. Conidiophores micronematous. Arthroconidia dark brown, thick-walled, smooth, sometimes rugose, globose, measuring 3.5-6 × 4.5-6 μm, single central septa, resulting from the differentiation of intercalary or terminal toruloid-like hyphal cells. Sexual morph unknown.

Culture characteristics.

On 6 weeks old PDA plates, colonies growing slowly, to 8 mm in diameter, cerebriform, irregular, raised centrally, often moist, deeply immersed into agar, pale pink, with scarce velvety, pale pink-hyaline, aerial, short hyphae and well-defined, small, white, and glossy margin; pale pink on reverse. After at least 2 months, colonies become fully mature and melanized, olivaceous brown-black on top and on reverse. On 6 weeks old MEA+10% NaCl plates, colonies growing slowly, to 25 mm in diameter, flat, circular, moist, pale pink, with prominent velvety pale pink-hyaline aerial short hyphae and with undulate margin; pale pink in reverse. After at least 2 months, colonies become fully mature and melanized, with velvety grey-white aerial short hyphae, filiform margin, olivaceous brown on top and in reverse. On 6-weeks-old DG-18 plates, colonies growing slowly, to 20 mm in diameter, flat, irregular, often moist, deeply immersed into agar, with velvety yellow-pale brown aerial short hyphae and with irregular undulate white margin; yellow-pale brown in reverse. After at least 2 months, colonies become fully mature and melanized, raised, rugose, olivaceous brown-black on top and in reverse.

Distribution.

Portugal.

Additional specimens examined.

Portugal, Coimbra (40°12'32"N, 8°25'38"W), isolated from a biodeteriorated limestone art piece in the Old Cathedral of Coimbra, I. Tiago, living culture E8, ibid. living culture E11, ibid. living culture E12, ibid. living culture E13, ibid. living culture E14, ibid. living culture E15, ibid. living culture E16, ibid. living culture E17, ibid. living culture E18; ibid. living culture E19.