Amblyseius myrtilli Papadoulis, Emmanouel and Kapaxidi, 2009

Khaustov, Vladimir A., 2020, Review of Amblyseius Berlese (Acari: Phytoseiidae) in Western Siberia, Russia, Acarologia 60 (4), pp. 769-805 : 795-798

Amblyseius myrtilli Papadoulis, Emmanouel and Kapaxidi

Amblyseius myrtilli Papadoulis, Emmanouel & Kapaxidi, 2009: 57 , Fig. 33.

World distribution — Greece ( Demite et al. 2020).

Material examined — six females, Russia, Tyumen Province, forest park Zatyumensky , 57°09 ′ N, 65°27 ′ E, 26 April 2019, A, Khaustov coll., from soil ; 2 females, Russia, Kurgan Province, vicinity of Zverinogolovskoe , 54°27 ′ N, 64°51 ′ E, 28 September 2019, A, Khaustov coll., from soil ; 2 females, Russia, Tyumen Province, vicinity of lake Kuchak , 57°21 ′ N, 66°03 ′ E, 27 April 2018, A, Khaustov coll., from soil .

Redescription — Female ( Figs. 21 , 22 , 23 , 24 , 25 , 27D ) (n = 4)

Idiosomal setal pattern – 10A:9B/JV–3:ZV.

Dorsal idiosoma ( Figs. 21A , 25A ) – Dorsal shield broadly oval, smooth, 362 (360-363) long and 262 (246-279) wide; with 19 pairs of setae (included r3 and R1), all setae smooth; length of setae: j1 24 (22-25), j3 43 (41-46), j4 6 (6), j5 6 (5-6), j6 6 (5-6), J2 7 (6-7), J5 11 (10-12), z2 13 (11-14), z4 9 (8-10), z5 6 (6), Z1 7 (6-7), Z4 89 (86-92), Z5 164 (156-173), s4 Characters Russia, Georgia Lithuania Characters Russia, Georgia Lithuania

Tyumen district (4) (1) (?) Tyumen district (4) (1) (?)

Dsl 353 (340-371) 515 348 z2 15 (15-16) 16 14

Dsw 232 (229-236) 294 219 z4 15 (13-16) 17 13

j1 26 (25-27) 22 25 z5 5 (5-6) - 6

j3 42 (40-44) 41 41 Z1 6 (5-6) 12 -

j4 6 (5-6) 7 6 Z4 91 (90-92) 95 89

j5 5 (5-6) 7 6 Z5 140 (132-147) 129 139

j6 5 (5-6) 7 5 Vsl 115 (113-117) - 114

J2 5 (5-6) 7 6 Vsw ZV2 93 (91-94) - 89

J5 9 (8-10) 6 9 JV5 66 (63-70) - 67

r3 18 (17-18) - 11 SgeIV 75 (74-80) - 67

R1 14 (13-14) - 10 StiIV 62 (60-64) - 58

s4 63 (62-67) 65 65 StIV 59 (58-59) - 56

S2 9 (8-10) - 8 Nbf 15-16 - 16

S4 8 (7-9) 9 8 Nbm 3 - 3

S5 8 (7-8) 9 -

60 (57-62), S2 11 (10-12), S4 10 (9-10), S5 11 (10-11); setae r3 15 (14-15) and R1 10 (9-11)

on lateral soft cuticle; dorsal shield with seven pairs of solenostomes gd (1, gd2, gd4, gd5, gd6, gd8, gd9) and 16 pairs of poroids.

Gnathosoma ( Figs. 22B, 22C, 22D, 22E ) – Anterior margin of epistome bump-like and smooth. Hypostomal groove with seven transverse rows of denticles, each row with two or three teeth; subcapitular setae h1 25 (24-26), h2 24 (23-24), h3 24 (23-24), palp coxal setae (pc) 28 (27-28). Chaetotaxy of palps: trochanter with two setae v1, v2; femur with five setae, thickened and apically spatulate antero-lateral al, three dorsal (d1, d2, d3) and one postero-lateral (pl); genu with six setae, antero-lateral setae (al1 and al2) thickened, three dorsal setae d (1, d2, d3) and one postero-lateral (pl); tibia with 14 setae, one antero-lateral (al), eight dorsal d1 – 8, two setae di-1, di-2, arise from the dorsal surface at the distal end, two ventral v (1, v2) and one postero-lateral (pl); tarsus with 15 setae (six simple d1, d2, d3, v1, v2, v3; nine stout setae with rounded tips di-1 to di- 9) and two-tined apotele ( Fig. 22C ).

Chelicera ( Figs. 22A , 25C ) – fixed digit 28 (27-30) long, with 3-4 teeth and pilus dentilis; movable digit 28 long, with two small teeth.

Ventral idiosoma ( Figs. 21B , 25B ) – Tritosternum with paired pilose laciniae 78, fused basally 25, columnar base 15-16 × 12 wide. Sternal shield smooth, 62 (59-63) long and 66 (63-67) wide, with three pairs of setae ST1 31, ST2 25 (24-25), ST3 26 (25-28) and two pairs of lyrifissures iv1, iv2. Setae ST4 23 (22-25) situated on small separate metasternal platelets, each with one pore iv3.

Genital shield smooth, 67 (66-71) wide at level of base of setae ST5 31 (29-32), para-genital poroids iv5 on soft cuticle.

Opisthosomatic venter with two pairs of elongated metapodal platelets, primary 24 (22-26) and accessory 18 (17-19) long; four pairs of setae, ZV1 16 (16-17), ZV3 8, JV4 12 (12-13), JV5

68 (67-68) long, all smooth, and five pairs of poroids.

Ventrianal shield pentagonal in shape, noticeably wider than genital shield, with reticulation in anterior part, 120 (116-123) long and 111 (106-116) wide at level of setae ZV2, with three pairs of pre-anal setae JV1 17 (16-18), ZV2 18 (16-19), JV2 24 (23-25), with small rounded preanal pores gv3 (distance between pores 46 (44-48)); para-anal setae 20 (19-21) and post-anal setae 20 (20-21).

Peritreme ( Figs. 21A , 22F , 25A ) – extends anteriorly to setae j1.

Spermatheca ( Figs. 22G , 25D ) – Calyx sacculus-like, 25 (23-26) long and at the opening

15 (12-16) wide; atrium C-shaped, connected without neck with calyx; major duct thick, minor duct visible in some specimens.

Legs ( Figs. 23 , 24 ) – Legs I 351 (349-353) and IV 327 (318-336) longer than legs II 270

(264-279) and III 257 (248-264). Chaetotaxy normal for phytoseiid mites: Leg I: coxa 0 0/1

0/1 0, trochanter 1 0/1 0/2 1, femur 2 3/1 2/2 2, genu 2 2/1 2/1 2, tibia 2 2/1 2/1 2. Leg II: coxa

0 0/1 0/1 0, trochanter 1 0/1 0/2 1, femur 2 3/1 2/1 1, genu 2 2/0 2/0 1, tibia 1 2/1 1/1 1. Leg

III: coxa 0 0/1 0/1 0, trochanter 1 0/1 0/2 1, femur 1 2/1 1/0 1, genu 1 2/1 2/0 1, tibia 1 1/1 2/1 1. Leg IV: coxa 0 0/1 0/0 0, trochanter 1 0/1 0/2 1, femur 1 2/1 1/0 1, genu 1 2/1 2/0 1, tibia 1

1/1 2/0 1.

Chaetotaxy of tarsi II-IV typical for Phytoseiidae and bears 18 setae 3 3/2 3/2 3 + mv, md.

Tarsus I with 37 setae, excluding apical sensorial setal cluster. Setae d3 22-24 with rounded tip, d4 30. Apical sensorial setal cluster (27D) includes 10 short setae of different shape.

Setae df-1 (12), df-3 (7-8) and df-7 (8-9) finger-shaped with blunt tips. Setae df-2 (7), df-5 (6),

df-6 (16) and df-8 (9-10) baculiform with rounded tips, setae df-8 usually curved. Setae df-4

(12-14), df-9 (9) spur-like with lobe-like tips, setae df-10 (6) situated between df-4 and df-9

also spur-like with acuminate tip. Measurements of macrosetae as follows: SgeII 31 (30-32),

SgeIII 32 (31-33), StiIII 25 (24-27), SgeIV 75 (72-80), StiIV 60 (59-60), StIV 55 (52-58). All macrosetae are acuminate.

Remarks — Amblyseius myrtilli is known only from Greece, on leaves of Vaccinium myrtillis (Ericaceae) and from litter under Juniperus sp. Morphological characteristics of

Siberian specimens are consistent with those provided in the original description ( Papadoulis et al., 2009). It is the second record of A. myrtilli in the world and the first report in Russia. The record of A. myrtilli in Russia is not accidental, since in Greece it was found in the mountains at an altitude of 1800 m. At this altitude, the climate is moderate and more or less similar to the southwestern Siberia.

Figures

Figure 21 Amblyseius myrtilli Papadoulis, Emmanouel and Kapaxidi, 2009, female: A – dorsal idiosoma; B – ventral idiosoma.

Figure 22 Amblyseius myrtilli Papadoulis, Emmanouel and Kapaxidi, 2009: A – chelicera, B – right palp, excepting tarsus, dorsal aspect, C – dorsal view of right palp tarsus, D – anterior margin of epistom, E – subcapitulum, F – peritrema, G – spermatheca.

Figure 23 Amblyseius myrtilli Papadoulis, Emmanouel and Kapaxidi, 2009, female: left legs I-IV, respectively, excluding tarsus, ventral aspect, macrosetae depicted in solid black.

Figure 24 Amblyseius myrtilli Papadoulis, Emmanouel and Kapaxidi, 2009, female: left tarsi I-IV, respectively, macrosetae depicted in solid black. A – dorsal aspect; B–D – ventral aspect.

Figure 25 Phase contrast (A, B) and DIC (C, D) micrographs ofAmblyseius myrtilli Papadoulis, Emmanouel and Kapaxidi, 2009: A – dorsal idiosoma of adult female, B – ventral idiosoma of female, C – chelicera of female, D – spermatheca.

Figure 27 Apical sensorial setal cluster area and setaed3 andd4 of tarsus I, female, right leg, dor- sal aspect. A – Amblyseius rademacheri Dosse, 1958, B – Amblyseius obtusus (Koch, 1839), C – Amblyseius omaloensis Gomelauri, 1968, D – Amblyseius myrtilli Papadouliset al., 2009.