Amphichorda kunmingensis Hong Yu

Wang, Zhi-Qin, Zhao, Jing, Dong, Quan-Ying, Wang, Yao, Lu, Ying-Ling, Luo, Run & Yu, Hong, 2024, Multi-locus molecular phylogenetic analysis reveals two new species of Amphichorda (Bionectriaceae, Hypocreales), MycoKeys 106, pp. 287-301 : 287-301

Amphichorda kunmingensis Hong Yu bis, Z. Q. Wang, Q. Y. Dong & Y. Wang sp. nov.

Fig. 4

Etymology.

Named from the location Kunming City where the species was collected.

Type.

China, Yunnan Province, Kunming City, Wild Duck Lake Forest Park, 16 July 2019, Hong Yu and Yao Wang (YHH AKYYH 200704, holotype; YFCC AKYYH 8414, ex-type).

Description.

Sexual morph: Undetermined. Asexual morph: Colonies on PDA attaining a diameter of 52–54 mm after a month at 25 ° C, white to pale grey, with low mycelial density, lanose. Hyphae hyaline, branched, smooth-walled, septate, 0.7–1.9 µm wide. Cultures readily produced phialides and conidia after 3 weeks on potato dextrose agar at room temperature. Phialides arising laterally from aerial hyphae, solitary, occasionally in simple whorls on lateral branches from the mycelia, basal portion cylindrical or fusiform, straight or irregularly bent, 6.1–17.5 × 1.4–2.9 µm. Conidia 2.3–4.2 × 1.6–3.0 µm, one-celled, smooth-walled, hyaline, globose to elliptical, single or aggregating in small heads at the apex of conidiogenous cells. Chlamydospores not observed.

Substrate.

Animal faeces.

Distribution.

China.

Other material examined.

China, Yunnan Province, Kunming City, Wild Duck Lake Forest Park, 16 July 2019, Hong Yu and Yao Wang (YHH AKYYH 200776, paratype; YFCC AKYYH 8487, ex-paratype).

Commentary.

Three species of Amphichorda were from China and A. yunnanensis was distributed in Yuxi City, Yunnan Province. The two new species in this study were from Kunming City, Yunnan Province. According to the phylogenetic tree, the new species, A. kunmingensis , forms a separate branch in Amphichorda and is sister to A. guana . However, it differs from A. guana by its smaller conidia. Although A. kunmingensis , A. excrementa and A. yunnanensis were all collected from Yunnan, their morphology was quite different (see Table 2 ). Amphichorda kunmingensis differs from A. excrementa in its usually curved and longer phialides (6.1–17.5 × 1.4–2.9 μm vs. 4.1–13.9 × 1.3–2.1 μm) and larger conidia (2.3–4.2 × 1.6–3.0 μm vs. 1.7–3.0 × 1.2–2.5 μm). Amphichorda kunmingensis differs from A. yunnanensis in the shape of its phialides and narrower conidia.

Figures

Figure 4. Morphology of Amphichorda kunmingensis A, B colony character on PDA medium after 30 d (A obverse B reverse) C – K conidiogenous cells and conidia. Scale bars: 2 cm (A, B); 20 µm (C – E, H); 10 µm (F – G, I – K).