Apiospora coryli S.J. Li & C.M. Tian, 2023

Li, Shuji, Peng, Cheng, Yuan, Rong & Tian, Chengming, 2023, Morphological and phylogenetic analyses reveal three new species of Apiospora in China, MycoKeys 99, pp. 297-317 : 297

Apiospora coryli S.J. Li & C.M. Tian sp. nov.

Fig. 3

Type.

China, Shanxi Province: Ankang City, Huoditang Forest Farm, on dead plant culms of Corylus yunnanensis , 16 July 2021, R. Yuan & S.J. Li, holotype BJFC-S1920, ex-type living cultures CFCC 58978, CFCC 58979 .

Etymology.

Named after the host from which it was isolated.

Description.

Asexual morph: Derived from sporulated cultures on PDA, hyphae hyaline, branched, septate, 1.1-5.2 µm diam. Conidiophores reduced to conidiogenous cells. Conidiogenous cells erect, aggregated in clusters on hyphae, hyaline to pale brown, smooth, doliiform to clavate or lageniform, 2.6-10.6 × 2.1-5.8 µm, mean ( ± SD): 5.5 ( ± 2.4) × 3.4 ( ± 1.1) µm (n = 50). Conidia brown to dark brown, globose to subglobose, oval or irregular, smooth to finely roughened, guttulate, usually with a longitudinal germ slit, 7.4-18.4 × 6.2-12.5 µm, mean ( ± SD): 10.8 ( ± 1.7) × 9.4 ( ± 1.3) µm, L/W = 0.8-1.6 (n = 50). Sexual morph: Undetermined.

Culture characteristics.

On PDA, colonies circular, flat, entire margin, thick and cottony, concentrically spreading with aerial mycelium, margin regular, reddish-brown pigment diffused into medium, surface dark yellowish-brown, reverse dark reddish-brown to yellowish-brown from the centre, mycelia white to pale umber, sporulation on hyphae, reaching 9 cm in 7 days at 25 °C.

Notes.

Strains of A. coryli constitutes a distinct clade, but there is poor support value in concatenated gene trees (Fig. 1 ). The most prominent distinguishing characteristic is the production of reddish-brown pigments on the culture medium.

Figures

Figure 1. Phylogram of Apiospora, based on combined ITS, LSU, tef 1 and tub 2 genes. ML bootstrap support values (≥ 50 %) and Bayesian posterior probability (≥ 0.90) are shown as first and second position above nodes, respectively. Strains from this study are shown in blue boxes, ex-type or ex-epitype cultures are indicated in bold face. Some branches were shortened according to the indicated mulipliers.

Figure 3. Apiospora coryli (CFCC 58978, ex-holotype culture) A leaf of host plant B colony on PDA C conidiomata formed in culture D, E conidiogenous cells giving rise to conidia F conidia. Scale bars: 1000 µm (C); 10 µm (D-F).