Brasilocypria alisonae, Almeida, Nadiny Martins De, Ferreira, Vitor Góis, Martens, Koen & Higuti, Janet, 2023

Almeida, Nadiny Martins De, Ferreira, Vitor Góis, Martens, Koen & Higuti, Janet, 2023, 5237, Zootaxa 5237, pp. 1-88 : 37-46

Brasilocypria alisonae gen. et spec. nov.

( Figs 20–26 )

Diagnosis. Cp suboval, CpD and CpV rather wide; LV overlapping RV, except in the middle of dorsal margin; anterior and posteroventral overlap the largest. Posteroventral tubercles in RV more prominent than anteroventral ones. A2 with natatory setae well-developed, short accompanying seta absent; male A2 with four-segmented endopodite and with t2 and t3 transformed into sexual bristles. Rpp and Lpp asymmetrical. First segment of Rpp stout, broad and long, with V-shaped dorsal margin and distally with a curved spinal edge; second segment subtriangular, with a curved dorsal margin and a rectangular dorso-distal edge. Lpp with shorter first segment, distally slightly tapering; second segment smoothly curved, second half elongated. T1 with one long and hirsute seta next to the two a-setae. T2 without d1 and d2 setae. T3 of male without dp and d2 setae; female only without d2-seta. CR with a long proximal seta. Hp with broad body; ls elongated, with parallel edges; ms consisting of two lobes, dorsal lobe broadly and irregularly curved, not reaching middle of ls, ventral lobe elongated with blunt point.

Differential diagnosis. Brasilocypria alisonae gen. et spec. nov. resembles Keysercypria deformis in the shape of the dorsal margin of the RV. But in the new species, the overlap of the LV on the RV is similar along all margins, except in the middle of dorsal side, while in K. deformis the overlap along the anterior side is much larger. In K. deformis , the lobe ms of the Hp is more than 2/3 the length of the ls, while in B. alisonae gen. et spec. nov., the ms is much shorter than the ls, i.e., ca. 1/2 the length of ls. The second segment of the Rpp in B. alisonae gen. et spec. nov. is short and has a weakly rounded distal margin and a dorsal margin which is nearly straight in its distal part, while in K. deformis this second segment is larger and has a sinuous margin. In addition, the h3-seta of the T2 is very large, which is typical of Keysercypria (see discussion).

Physocypria granadae Hartmann, 1959 and K. schubarti also have a similar morphology of the Hp as in B. alisonae gen. et spec. nov., but the prehensile palps of the first species are more slender, while the second segment of the Rpp is much more robust than in B. alisonae gen. et spec. nov. Furthermore, the dorsal margin of the LV of P. granadae is straighter than in B. alisonae gen. et spec. nov., and in K. schubarti the greatest overlap of the LV over the RV is situated along the anterior margin, while this anterior overlap is much less pronounced in B. alisonae gen. et spec. nov.

Brasilocypria alisonae gen. et spec. nov. has a similar Cp shape as in B. pea gen. et spec. nov., but the dorsal margin is straighter in the former species, while B. pea gen. et spec. nov. is generally longer.

Material examined. Type locality: South Matogrossense Pantanal, Miranda II River ( PAN 66), in Eichhornia azurea , Ludwigia sp. , Pistia sp. and Salvinia spp. Coordinates: 19°2′7.7′′ S, 57°18′49′′ W.

Type material: Holotype: A male, with soft parts dissected in glycerine in a sealed slide and with valves stored dry in a micropaleontological slide ( MZUSP 43123 ).

Allotype: A female, dissected and stored as the holotype ( MZUSP 43124 ).

Paratypes: Two males dissected and stored as the holotype ( MZUSP 43125 , MZUSP 43126 ) . Two male carapaces stored dry in micropaleontological slides ( MZUSP 43127 , MZUSP 43128 ) .

One female dissected and stored as the holotype ( MZUSP 43129 ) . Three female carapaces stored dry in micropaleontological slides ( MZUSP 43130 , MZUSP 43131 , MZUSP 43132 ) .

Etymology: This species is named in honour of Prof. Alison Smith (Kent University, Ohio, USA) in honour of her significant contribution to the taxonomy and ecology of North American non-marine ostracods, both fossil and recent.

Other material illustrated: South Matogrossense Pantanal : One male dissected and stored as the holotype ( MZUSP 43133 ) from Curva Doleque Road , lake 4 (PAN 08) .

One male dissected and stored as the holotype ( MZUSP 43134 ) from Corumba Road , pool 2 (PAN 14) .

One male dissected and stored as the holotype ( MZUSP 43135 ) from Corumba Road , lake 4 (PAN 18) .

One male dissected and stored as the holotype ( MZUSP 43136 ) and three male carapaces stored dry in micropaleontological slides ( MZUSP 43137 , MZUSP 43138 , MZUSP 43139 ) from Camp Road , pool 4 (PAN 31) . One female dissected and stored as the holotype ( MZUSP 43140 ) and one female carapace stored dry in a micropaleontological slide ( MZUSP 43141 ) from Camp Road, pool 4 (PAN 31) .

Other material examined: South Matogrossense Pantanal : Two males dissected and stored as the holotype ( MZUSP 43142 , MZUSP 43143 ) from Curva Doleque Road , lake 4 (PAN 08) . Two females dissected and stored as the holotype ( MZUSP 43144 , MZUSP 43145 ) from Curva Doleque Road, lake 4 (PAN 08) .

One male dissected and stored as the holotype ( MZUSP 43146 ) from Corumba Road , lake 4 (PAN 18) .

One male dissected and stored as the holotype ( MZUSP 43147 ) from BEP wetland 2 (PAN 27) .

One male dissected and stored as the holotype ( MZUSP 43148 ) from Camp Road , pool 4 (PAN 31) .

Measurements of illustrated specimens. See Table 2 .

Description of male. Valves with suboval shape, with greatest height in middle of valves. LVi ( Figs 20A, C–D ) with narrow calcified anterior and posterior inner lamella; an inwardly displaced inner list running along the anterior margin, ending in an internal ventral tooth; tubercle-sockets displaced along anteroventral and posteroventral margins.

RVi ( Figs 20B, E–F ) with narrow calcified anterior and posterior inner and an inwardly displaced inner list along the anterior margin, disappearing in the anteroventral margin; strong marginal tubercles along posteroventral margin, weakly along middle and anteroventral margins.

CpRl ( Fig 20G ) with a suboval shape, with greatest height in middle; LV overlapping RV along all margins, except in middle of dorsal side. CpD ( Fig 20H ) and CpV ( Fig 20I ) with greatest width in middle, external surface with shallow pits ( Fig 20J ).

Only the differences with the male of Brasilocypria pea gen. et spec. nov. are described here.

A1 (not illustrated) third segment with two apical setae: ventrally with one short seta reaching middle of fifth segment and dorsally with one short hirsute seta reaching tip of fifth segment.

A2 (not illustrated) exopodite a small plate with one long hirsute seta (reaching tip of the terminal segment) and two shorter, unequally long setae (the long ca. 10x length of short). Terminal segment with one g-seta ca. 1/2 length of y3.

Md-palp (not illustrated) with second segment with stout hirsute β-seta (ca. 1/2 of the length of the third segment).

Mx1(not illustrated) first endite short with two basal setae(one short and one long, about6x length of previous one).

T1 with protopodite ( Fig 21A ) and endopodite (asymmetrical prehensile palps) ( Figs 21B–C ). Protopodite apically with a group of 14 unequal and hirsute setae; two short smooth a-setae, one smooth b-seta, one long and smooth d-seta (6x length of b-seta), and one long and hirsute seta next to the two a-setae (almost 2x length of d-seta). Rpp ( Fig 21B ) with first segment stout, broad and long, with V-shaped dorsal margin and distally with a curved spinal edge. Lpp ( Fig 21C ) with shorter first segment, distally slightly tapering; second segment smoothly curved, second half elongated.

T2 (not illustrated) first endopodal segment with one apical hirsute e-seta, reaching middle of third endopodal segment. Second endopodal segment with one apical hirsute f-seta, almost reaching tip of third endopodal segment. Third endopodal segment with one subapical hirsute g-seta, reaching beyond tip of terminal segment. Terminal segment with h3-seta slightly shorter than h1.

T3 (not illustrated) first segment without dp-seta and with one short d1-seta (ca. 1/4 of length of segment).

CR ( Fig 21D ) well-developed and robust, with long proximal Sp-seta (ca. 2/3 of ramus), subapically with serrated Gp-claw, apically with long serrated Ga-claw and short and smooth Sa-seta.

Hp ( Fig 21E ) with broad body; ls elongated, with parallel edges; ms consisting of two lobes, ventral lobe broadly and irregularly curved, not reaching middle of ls, ventral lobe elongated with blunt point.

Description of female. Only the differences with the male of Brasilocypria alisonae gen. et spec. nov. are described here.

LVi ( Figs 22A, C–D ) and RVi ( Figs 22B, E–F ) higher than male. RVi with tubercles along posteroventral margin, disappearing in middle and few tubercles next to anterior side. CpRl ( Fig 22G ) as in the male. CpD ( Fig 22H ) and CpV ( Fig 22I ) with greatest width behind middle, external surface with shallow pits and setae ( Fig 22J ).

A2 (not illustrated) exopodite with two short setae and with long hirsute seta reaching tip of second endopodal segment. Third endopodal segment apically with three claws (G1, G2, G3) and three setae (z1, z2, z3).

Mx1 (not illustrated) first endite short with two basal setae (one short and one long, about 3x length of previous one).

T1 ( Fig 23A ) with protopodite apically with a group of 15 unequal and hirsute setae; two smooth a-setae, one short and smooth b-seta, one long and smooth d-seta (ca. 7x length of b-seta), and one long and hirsute seta next to the two a-setae (almost 2x length of d-seta). Endopodite apically with one long and two short setae (one ca. 2/3 length of the long and one 1/2 length of the long).

T3 (not illustrated) first segment with one long and hirsute dp-seta and one short d1-seta (the latter ca. 1/2 of length of dp).

CR ( Fig 23B ) well-developed and robust, with long proximal hirsute Sp-seta (ca. 2/3 of ramus), subapically with serrated Gp-claw, apically with long serrated Ga-claw and short and smooth Sa-seta.

Remarks: As this species occurs in more than one locality in South Matogrossense Pantanal, we also illustrate valves of males and one female ( Fig 24C, D ) and carapaces of a male and female ( Fig 25 ) from other lakes of the South Pantanal Matogrossense, to show the similarities among them. We also illustrate the outline of Hp and prehensile palps of four males to show some variabilities between specimens ( Fig 26 ). For instance, the ls of the Hp is robust in some specimens ( Figs 26A–B, I–J, M–N ), and slender in others ( Figs 26E–F ). The distal part of the second segment of the Lpp can be distally pointed ( Fig 26O ) or rounded ( Figs 26 G, K ). Also, the basal part of the first segment of the Lpp is wider in some specimens ( Figs 26H, P ) and narrower in others ( Figs 26D, L ).

Ecology and distribution. Brasilocypria alisonae gen. et spec. nov. was recorded only from South Matogrossense Pantanal, associated with different species of macrophytes. The water temperature recorded was 19 to 24°C. The pH ranged from 6 to 8.1, while the range of electrical conductivity was between 140 and 415 μS. cm-1 and the value recorded of dissolved oxygen was 4.5 mg. L- 1 (see Table 1 ).

Figures

FIGURE 20. Brasilocypria alisonae gen. et spec. nov. ♁ from South Matogrossense Pantanal, Miranda II River, (PAN 66). A. LVi (MZUSP 43123). B. RVi (MZUSP 43123). C. LVi, detail of posterior part (MZUSP 43123). D. LVi, detail of anterior part (MZUSP 43123). E. RVi, detail of anterior part (MZUSP 43123). F. RVi, detail of posterior part (MZUSP 43123). G. CpRl (MZUSP 43127). H. CpD (MZUSP 43128). I. CpV (MZUSP 43128). J. CpRl, detail of anterior part showing the surface of Cp (MZUSP 43127). Scale bars: A–B, H = 250 μm; C–F = 100 μm; G, I = 300 μm; J = 50 μm. Arrow indicates anterior direction.

FIGURE 21. Brasilocypria alisonae gen. et spec. nov. ♁ from South Matogrossense Pantanal, Miranda II River, (PAN 66). A. T1 (MZUSP 43125). B. Rpp (MZUSP 43123). C. Lpp (MZUSP 43123). D. CR (MZUSP 43125). E. Hp (MZUSP 43123). Scale bars: A–E = 50 μm.

FIGURE 22. Brasilocypria alisonae gen. et spec. nov. ♀ from South Matogrossense Pantanal, Miranda II River, (PAN 66). A. LVi (MZUSP 43124). B. RVi (MZUSP 43124). C. LVi, detail of posterior part (MZUSP 43124). D. LVi, detail of anterior part (MZUSP 43124). E. RVi, detail of anterior part (MZUSP 43124). F. RVi, detail of posterior part (MZUSP 43124). G. CpRl (MZUSP 43130). H. CpD (MZUSP 43131). I. CpV (MZUSP 43132). J. CpRl, detail of anterior part showing the surface of Cp (MZUSP 43130). Scale bars: A–B = 250 μm; C–F = 200 μm; G–I = 300 μm; J = 50 μm. Arrow indicates anterior direction.

FIGURE 23. Brasilocypria alisonae gen. et spec. nov. ♀ from South Matogrossense Pantanal, Miranda II River, (PAN 66). A. T1 (MZUSP 43124). B. CR (MZUSP 43124). Scale bars: A–B = 50 μm.

FIGURE 24. Brasilocypria alisonae gen. et spec. nov. from South Matogrossense Pantanal. A–B, E–H. ♁. C–D. ♀. A–D. Camp Road, pool 4 (PAN 31): A. LVi (MZUSP 43136). B. RVi (MZUSP 43136). C. LVi (MZUSP 43140). D. RVi (MZUSP 43140). E–F. Curva Doleque Road, lake 4 (PAN 08): E. LVi (MZUSP 43133). F. RVi (MZUSP 43133). G–H. Corumbá Road, lake 4 (PAN 18): G. LVi (MZUSP 43135). H. RVi (MZUSP 43135). Scale bars: A–H = 250 μm. Arrow indicates anterior direction.

FIGURE 25. Brasilocypria alisonae gen. et spec. nov. from South Matogrossense Pantanal, Camp Road, pool 4 (PAN 31). A–D. ♁. E–H. ♀. A. CpRl (MZUSP43137). B. CpD (MZUSP 43138). C. CpV (MZUSP 43139). D. CpRl, detail of anterior part showing the surface of Cp (MZUSP43137). E. CpRl (MZUSP 43141). F. CpD (MZUSP 43141). G. CpV (MZUSP 43141). H. CpV, detail of anterior part showing the surface of Cp (MZUSP 43141). Scale bars: A–C, E–F = 250 μm; D, H = 100 μm.Arrow indicates anterior direction.

FIGURE 26. Brasilocypria alisonae gen. et spec. nov. ♁ from South Matogrossense Pantanal. A–D. Camp Road, pool 4 (PAN 31) (MZUSP 43136). E–H. Corumbá Road, pool 2 (PAN 14) (MZUSP 43134). I–L. Curva Doleque Road, lake 4 (PAN 08) (MZUSP 43133). M–P. Corumbá Road, lake 4 (PAN 18) (MZUSP 43135). A–B; E–F; I–J; M–N. Hp. C, G, K, O. Rpp. D, H, L, P. Lpp. Scale bars: A–P = 50 μm.