Brinkmanniella simplex, Diez & Monnens & Wuyts & Brendonck & Reygel & Schmidt-Rhaesa & Artois, 2023
|
publication ID |
https://doi.org/ 10.1007/s13127-023-00623-w |
|
publication LSID |
lsid:zoobank.org:pub:4D2516BA-19CF-46C6-8D96-F17DD505B4FF |
|
persistent identifier |
https://treatment.plazi.org/id/0C021059-6F7F-FF80-1D20-FD30FB44FC82 |
|
treatment provided by |
Felipe |
|
scientific name |
Brinkmanniella simplex |
| status |
|
Brinkmanniella Luther, 1943 View in CoL
Brinkmanniella simplex sp. n. Diez, Reygel & Artois ( Fig. 10a, b, e View Fig )
urn:lsid:zoobank.org:act:62C1E916-AE14-4571-AFD1-443E047266C3
Brinkmanniella sp. 1 in Diez et al. (2023)
Material and distribution. Cuba: Observations on one live animal, whole mounted afterwards, designated holotype ( ZMH, No. V13683 ), collected in Bueycabón (type locality) (25 May 2018); on the alga Digenea simplex , 0.5 m deep, salinity 32 ‰.
Etymology. Species named after the simple morphology of its stylet. Lat. simplex : simple.
Diagnosis. Species of Brinkmanniella with a funnel-shaped 60-µm-long stylet, which is 29 µm wide proximally and 3 µm wide distally.
Description. The live animal is about 0.6 mm long, unpigmented, with a pair of eyes ( Fig. 10a View Fig : e). The pharynx ( Fig. 10a View Fig : ph) has a diameter of about 30% of the body length of the live specimen and is located caudally.
The testes ( Fig. 10a View Fig : t) are located in the second body half, caudal to the ovaries. The vasa deferentia open separately into the copulatory bulb, where they join and form the single seminal vesicle ( Fig. 10a View Fig : sv). The copulatory bulb is located beside the pharynx and apart from the large and rounded seminal vesicle. It also encloses the prostate vesicle which is connected to the stylet. The funnel-shaped stylet ( Fig. 10a View Fig : st, b, e) is 60 µm long, 29 µm wide proximally, and 3 µm wide distally.
The ovaries ( Fig. 10a View Fig : ov) are located anterior to the testes in the anterior half of the body.
Brinkmanniella tenebrosa sp. n. Diez, Reygel & Artois ( Fig. 10c, d, f View Fig )
urn:lsid:zoobank.org:act:4D693C04-8A69-4B99-A7E2-5D05FCB82F70
Brinkmanniella sp. 2 in Diez et al. (2023)
Material and distribution. Cuba: Observations on live animals. Four whole mounts from Bueycabón (type locality) (6 February 2018), one of which is designated holotype ( ZMH, No. V 13684) and the others paratypes (HU, No. 862–864), and one specimen used for molecular analyses; on leaves of T. testudinum and the alga Galaxaura rugosa , 0.5 m deep, salinity 33‰.
Etymology. The epithet refers to the dark pigmentation of the animal. Lat. tenebrosus: dark.
Diagnosis. Species of Brinkmanniella darkly pigmented, with a ~ 59-µm-long tubular stylet, which is ~ 17 µm wide proximally and ~ 7 µm wide distally.
Description. The live specimens are about 0.8 mm long, darkly pigmented, with a pair of eyes ( Fig. 10c View Fig : e). Adenal rhabdite tracts present, with the cell bodies caudal to the eyes, their necks in between the eyes and opening terminally at the anterior end of the body. The pharynx ( Fig. 10c View Fig : ph) is located at 75%. It has a diameter of 20% of the body length in the live animals.
Organisation of the genital and atrial organs as in B. simplex sp. n. The funnel-shaped stylet ( Fig. 10d, f View Fig ) is 55–63 µm long (x = 59 µm; n = 3), 16–18 µm wide proximally (x = 17 µm; n = 3), and 6–9 µm wide distally (x = 7 µm; n = 3).
The globular ovaries ( Fig. 10c View Fig : ov) are located just caudal to the eyes and lie in the anterior body half.
Kymocarens Ehlers & Ehlers, 1981
Kymocarens morrisi sp. n. Diez, Reygel & Artois
( Fig. 11 View Fig )
urn:lsid:zoobank.org:act:78F39A2A-509D-4DC5-9569-990DD14830FE
Kymocarens sp. in Diez et al. (2023)
Material and distribution. Cuba: Observations on live animals, whole mounted afterwards. Seven whole mounts, one of which is designated holotype ( ZMH, No. V13685 ) and the others paratypes (HU, No. 865–870), and eight serially sectioned specimens (UH XIX.3.50, XIX.4.01– XIX.4.07) collected in Aguadores (Type locality) (24 January 2017); intertidal, upper 20 cm of medium-grained sand, salinity 35‰. One whole mount from Belmares , Bahía de Santiago de Cuba (January 31, 2017) (UH XIX.4.08); intertidal, upper 10 cm of coarse shell gravel, salinity 35‰. One whole mount from Juraguá (February 9, 2020) (UH XIX.4.09); intertidal, upper 20 cm of medium-coarse sand, salinity 33‰. One whole mount from Chivirico , near Hotel Guamá , Guamá (October 21, 2020) (UH XIX.4.10); sublittoral, 0.8 m deep, fine-grained and silty sand, surrounded by the turtlegrass Thalassia testudinum , salinity 33 ‰.
Etymology. Species dedicated to Prof. Dr Humberto Joaquín Morris Quevedo (Universidad de Oriente, Santiago de Cuba, Cuba), nutraceutical specialist and dear friend of the first author.
Diagnosis. Species of Kymocarens with a pair of eyes. Tubular stylet ~ 81 µm long, with a longitudinal slit in its proximal end and a 90° bend at its mid-length. Internal bursal vesicle surrounded by a parenchymal sheath.
Description. The live animals are 0.5–0.7 mm long, translucent, with a pair of eyes ( Fig. 11a View Fig : e). Adenal rhabdite tracts present ( Fig. 11a View Fig : ar), with the cell bodies caudal to the eyes, their necks in between the eyes and opening terminally at the anterior tip of the body. These rhabdites are 5–12 µm long (x = 7 µm; n = 11), measured on sections. The syncytial and fully ciliated epidermis is 6–9 µm thick dorsally, with rhabdites 5–7 µm long (x = 6 µm; n = 15). Ventrally, the epidermis is 2–3 µm thick, containing few ~ 2 µm-long rhabdites. The cilia are 3–4 µm long. Two types of vacuoles are present in the epidermis: some translucent and some filled with a granular secretion. The caudal body end is differentiated into a tail plate ( Fig. 11a View Fig : tp), with conspicuous adhesive glands.
The pharynx ( Fig. 11a View Fig : ph, b) is situated at 50%. At least four types of glands open into the pharyngeal lumen. These glands are, from distal to proximal, coarse-grained eosinophilic (stained reddish) ( Fig. 11b View Fig : phg1), coarse-grained basophilic (stained dark blue-black) ( Fig. 11b View Fig : phg2), and two fine-grained eosinophilic ones (stained brownish and yellowish, respectively) ( Fig. 11b View Fig : phg3 and phg4). The musculature of the pharynx consists of a layer of longitudinal muscles just outside of the septum ( Fig. 11b View Fig : lm1) and continuous with the longitudinal muscles of the prepharyngeal cavity. Apart from the radial muscles, we did not observe any other musculature inside the pharynx septum. Radial muscles run between the internal and external walls of the pharynx ( Fig. 11b View Fig : rm). The pharynx lumen is surrounded by a thin, anucleated epithelium ( Fig. 11b View Fig : ep), a longitudinal ( Fig. 11b View Fig : lm2), and circular muscle layer ( Fig. 11b View Fig : cm). The distal lips of the pharynx are ciliated ( Fig. 11b View Fig : ci); cilia 2 µm long. The distal opening of the pharynx lumen is surrounded by a sphincter ( Fig. 11b View Fig : sph2). The prepharyngeal cavity ( Fig. 11b View Fig : ppc) is lined with a thin, anucleated epithelium and an external layer of longitudinal muscles. A layer of denser parenchyma ( Fig. 11b View Fig : pa) surrounds the prepharyngeal cavity outside of the longitudinal muscle layer. The mouth ( Fig. 11b View Fig : m) is surrounded by a sphincter ( Fig. 11b View Fig : sph1).
The testes ( Fig. 11a View Fig : t) are located ventrally, rostrolaterally from the pharynx. The vasa deferentia form the seminal vesicles ( Fig. 11a, c View Fig : sv) caudal to the pharynx. The seminal vesicles fuse just before entering the copulatory bulb. They are lined by a nucleated epithelium. The prostate vesicle ( Fig. 11a, c View Fig : pv) is globular and contains medium-grained, eosinophilic secretion. We did not observe a muscle layer surrounding the prostate vesicle. The tubular stylet ( Fig. 11a, c View Fig : st, d–e) is 66–89 µm long (x = 81 µm; n = 9) and 8–15 µm wide proximally (x = 12 µm; n = 9). A longitudinal slit is present in its proximal end and the stylet is curved over 90° at its midpoint. The male atrium is lined by a nucleated epithelium and a layer of longitudinal muscles. It enters the common genital atrium anteriorly ( Fig. 11c View Fig : ca).
The paired vitellaria ( Fig. 11a, c View Fig : vi) extend dorsally along both sides of the body, from the eyes to the caudal body end. The ovaries ( Fig. 11a, c View Fig : ov) are situated caudally in the ovovitellaria. They are oval shaped, with the oocytes organised in a row. Each oviduct proximally forms a seminal receptacle ( Fig. 11c View Fig : sr), proximally of which a bundle of fine-grained, eosinophilic glands enter the oviduct ( Fig. 11c View Fig : eg2). Both oviducts then unite to form the female duct, which receives two types of female glands: fine-grained, eosinophilic ones proximally ( Fig. 11c View Fig : eg1) and coarse-grained, basophilic ones distally ( Fig. 11c View Fig : bg). The oviducts and female duct are lined with a nucleated epithelium; muscles were not observed. The female duct enters the common genital atrium caudally. The bursa ( Fig. 11a, c View Fig : b) is connected to the common genital atrium through its dorsal wall. This bursa is filled with sperm and a fine-grained, eosinophilic secretion. It is lined by a high, nucleated epithelium (~ 5 µm thick) and a circular muscle layer. The bursa is lined by longitudinal muscles in its distal half and a layer of dense parenchyma more proximally (~ 4 µm thick) ( Fig. 11c View Fig : pa). The common genital atrium is lined by a nucleated epithelium and a layer of longitudinal muscles. The common gonopore ( Fig. 11c View Fig : cg) is situated at 70% and surrounded by a sphincter ( Fig. 11c View Fig : sph).
| ZMH |
Zoologisches Museum Hamburg |
| V |
Royal British Columbia Museum - Herbarium |
No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.