Canariomyces arenarius (Mouch.) X. Wei Wang & Houbraken

Juxu, Rong, Phukhamsakda, Chayanard & Boonmee, Saranyaphat, 2024, Identification of a new species and three new records of Chaetomiaceae associated with commercial herbal teas in northern Thailand, Phytotaxa 653 (2), pp. 134-154 : 144-145

Canariomyces arenarius (Mouch.) X. Wei Wang & Houbraken , in Wang et al., Stud. Mycol. 93: 189 (2019) ( Fig. 4 ), new host record

Index Fungorum number: IF829846, Facesofungi number: FoF15767

Culture characteristics:—Colonies growing on PDA, reaching 3.5 cm diam., in 4 weeks at 25 °C in the dark condition, irregular, slightly effuse, hairy, mycelium radiating outwards, with undulate to fimbriate edge, white grey hyphae on surface with aerial white conidia, dark to black at reverse and margin. Mycelium superficial, partly immersed, hyaline, brown to dark brown, branched, septate, smooth-walled. Conidia produced in culture after one month.

Sexual morph: Not produced in culture during two months old. Asexual morph on PDA culture: Conidiophores reduced to conidiogenous cells developed on hyphae, monoblastic, laterally producing conidia. Conidia 3–6 × 3–4 µm (x = 4 × 3 µm, n = 20), born laterally, terminally or intercalary on the aerial hyphae, globose to subglobose, broad clavate to obovoid, hyaline to light brown, aseptate, smooth-walled.

Material examined:— THAILAND, Chiang Mai Province, Muang, Warorot market, black goji berry tea ( Lycium ruthenicum , Solanaceae ), 2 March 2023, Saranyaphat Boonmee, HBT43B (MFLU 24–0082, dried culture, new host record), living cultures (MFLUCC 24–0065); ibid., red goji berry tea ( Lycium barbarum L., Solanaceae ), 2 March 2023, Saranyaphat Boonmee, HBT46 (MFLU 24–0083, dried culture, new host record), living cultures MFLUCC 24–0066.

Notes:—Our two isolates, MFLUCC 24–0065 and MFLUCC 24–0066, were isolated from herbal tea products, i.e. black goji berry tea ( Lycium ruthenicum ) and red goji berry tea ( Lycium barbarum ) purchased from a local market in Chiang Mai, Thailand. Phylogenetic analysis based on a combined ITS, LSU, TUB2 and RPB2 sequence dataset indicated our isolates grouped with the ex-type strain of Canariomyces arenarius (CBS 507.74) which was isolated from desert soil in Egypt ( Mouchacca 1973), with 93% ML and 0.98 PP statistical support ( Fig. 3 ). Additionally, our isolates ( Fig. 4 ) share identical asexual morph characteristics with Ca. arenarius in the features of conidiophores reduced to conidiogenous cells on hyphae, monoblastic and hyaline to light brown, aseptate, globose to subglobose conidia ( Wang et al. 2019). Therefore, we identified the two isolates as a new record for Ca. arenarius from commercial herbal teas of Solanaceae in northern Thailand.

Figures

FIGURE 3. Phylogram generated from RAxML analysis based on combined ITS, LSU, RPB2 and TUB2 sequence data of Canariomyces taxa. Madurella pseudomycetomatis isolates CBS 129177T and CBS 217.55 are selected as the outgroup taxa. Bootstrap support values for ML values equal to or>70% and PP values equal to or>0.95 are shown as ML/PP above the nodes. Newly generated sequences in this study are in blue. Type strains are indicated in bold.

FIGURE 4. Canariomyces arenarius (MFLUCC 24–0066, new host record). a Red goji berry tea sample. b, c Culture colony grown on PDA from the surface and reverse after one month. d Top view of white conidial colonies in culture. e Hyphae in culture. f–i Developing short conidiophores and conidiogenous cells with apical conidia. Scale bars: d = 500 µm, e–i = 10 µm.