Colacogloea hydrangeae Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 132

Colacogloea hydrangeae Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828863. Fig. 19O .

Etymology: the specific epithet hydrangeae refers to Hydrangea , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical and ellipsoidal, 2.7–4.1 × 5.7– 10.9 μm and single, budding is polar ( Fig. 19O ), a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish cream, butyrous, smooth with partly wrinkled, glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, trehalose, melezitose (delayed), D-glucosamine, ethanol, ribitol (delayed), D-mannitol, Dglucitol and salicin are assimilated as sole carbon sources. Galactose, L-sorbose, cellobiose, lactose, raffinose, melibiose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, Lrhamnose, methanol, glycerol, erythritol, galactitol, Methyl-α- Dglucoside, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine and ethylamine hydrochloride are assimilated as sole nitrogen sources. Cadaverine dihydrochlorideare is not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is weak. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Co. hydrangeae differs from its closely related species Co. rhododendri in its inability to assimilate glycerol and its ability to assimilate salicin ( Table S1.38 ).

Typus: China, Lulang county, Tibet, obtained from a leaf of Hydrangea heteromalla, Sep. 2004 , Q.-M. Wang (holotype CGMCC 2.2798 T preserved in a metabolically inactive state, ex-type CBS 15463 = XZ46B3).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.