Cylindrocladiella parvispora N.Q. Pham, T.Q. Pham & M.J. Wingfield

Pham, Nam Q., Barnes, Irene, Chen, ShuaiFei, Pham, Thu Q., Lombard, Lorenzo, Crous, Pedro W. & Wingfield, Michael J., 2018, New species of Cylindrocladiella from plantation soils in South-East Asia, MycoKeys 32, pp. 1-24 : 5-8

Cylindrocladiella parvispora N.Q. Pham, T.Q. Pham & M.J. Wingfield sp. nov. Figure 5

Etymology.

Name refers to the small conidia produced by this species.

Type material.

VIETNAM. Tuyen Quang Province, from soil in Acacia hybrid plantation, Nov. 2013, N.Q. Pham & T.Q. Pham, herbarium specimen of dried culture, PREM 62167 (holotype), CMW 47197 = CBS 143554 (ex-type culture).

Description.

Sexual morph not observed. Conidiophores dimorphic, penicillate and subverticillate, mononematous and hyaline. Penicillate conidiophores comprising a stipe, a penicillate arrangement of fertile branches, a stipe extension and a terminal vesicle; stipe septate, hyaline, smooth, 67-107 × 3-6.5 µm; stipe extension aseptate, straight, 112.5-141 µm long, thick-walled with one basal septum, terminating in thin-walled, fusoid to cylindrical vesicles, 4.5-6.5 µm wide. Penicillate conidiogenous apparatus with primary branches aseptate, 10.5-25 × 2-4 µm, secondary branches aseptate, 7.5-17 × 2-3 µm, each terminal branch producing 2-4 phialides; phialides doliiform to reniform to cymbiform, hyaline, aseptate, 7.5-13 × 2-3 µm, apex with minute periclinal thickening and collarette. Subverticillate conidiophores in moderate numbers, comprising of a septate stipe and primary branches terminating in 2-4 phialides; primary branches straight, hyaline, 0-1-septate, 15.5-27 × 2.5-4 µm; phialides cymbiform to cylindrical, hyaline, aseptate, 13.5-41 × 2.5-6 µm, apex with minute periclinal thickening and collarette. Conidia cylindrical, rounded at both ends, straight, 1-septate, (8 –)10– 12(-13) × 2-2.5 µm (av. = 11 × 2 µm), frequently slightly flattened at the base, held in asymmetrical clusters by colourless slime.

Culture characteristics.

Colonies buff to honey to isabelline on the surface and umber to sepia in reverse on MEA after 7 d; smooth to undulate margin; abundant aerial mycelium especially in the middle; chlamydospores moderate, arranged in chains. Optimal growth temperature at 25 °C, no growth at 5 °C and 35 °C; after 7 d, colonies at 10 °C, 15 °C, 20 °C, 25 °C and 30°C reached 5.5 mm, 23.4 mm, 43.8 mm, 63.6 mm and 49.2 mm, respectively.

Distribution.

Tuyen Quang, Vietnam

Additional material examined.

VIETNAM. Tuyen Quang Province, from soil in Acacia hybrid plantation, Nov. 2013, N.Q. Pham & T.Q. Pham, PREM 62168, culture CMW 47207 = CBS 143555; ibid., PREM 62169, culture CMW 47208 = CBS 143556.

Notes.

Cylindrocladiella parvispora is phylogenetically closely related to Cy. arbusta , Cy. natalensis and Cy. obpyriformis . Conidia of Cy. parvispora are slightly smaller than those of Cy. arbusta , Cy. natalensis and Cy. obpyriformis (Table 2).