Diaporthe longicicola Y.H. Gao & L. Cai, Fungal Biology 119(5): 303 (2015)

Long, Hui, Zhang, Qian, Hao, Yuan-Yuan, Shao, Xian-Qiang, Wei, Xiao-Xing, Hyde, Kevin D., Wang, Yong & Zhao, De-Gang, 2019, Diaporthe species in south-western China, MycoKeys 57, pp. 113-127 : 113

Diaporthe longicicola Y.H. Gao & L. Cai, Fungal Biology 119(5): 303 (2015) Figure 5

Description.

Colonies on PDA attaining 9 cm diam. in 10 days; coralloid with feathery branches at margin, adpressed, without aerial mycelium, without numerous irregularly zonated dark stromata, isabelline becoming lighter towards the margin; reverse similar to the surface with zonations more apparent. Conidiomata pycnidial and multilocular, scattered, abundant on PDA after 20 d, subglobose or irregular, 1.5-1.8 mm diam., ostiolate and up to 1 mm long. Conidiophores formed from the inner layer of the locular wall, densely aggregated, hyaline to pale yellowish-brown, cylindrical, tapering towards the apex, 15-25 × 1.5-2 μm. Alpha conidia abundant, ellipsoid to fusiform, apparently biguttulate, hyaline, 6-9 × 2-3 μm. Beta conidia scarce to abundant, flexuous to J-shaped, hyaline, 25.5-35.5 × 1-2.5 μm.

Habitat and distribution.

Isolated from leaves of Camellia sinensis in Duyun, Guizhou Province, China

Notes.

Phylogenetic analyses ( Figures 1 , 2 ) indicated that GUCC 9146 has a close relationship with D. longicicola , D. rosicola , D. eres and D. cotoneastri . Morphological comparison indicated that this strain was most similar to D. longicicola but not a related species by the width of alpha conidia and length of beta conidia ( Udayanga et al. 2014; Gao et al. 2015).

Figures

Figure 1. Parsimonious tree obtained from a combined analyses of an ITS, β - tubulin, calmoudulin and tef 1 sequence dataset. MP, ML above 50 % and BPP values above 0.90 were placed close to topological nodes and separated by " / ". The bootstrap values below 50 % and BPP values below 0.90 were labelled with " - ". The tree is rooted with Valsa ambiens (CFCC 89894). The branch of our new Diaporthe species is in pink.

Figure 2. Parsimonious tree obtained from a combined analyses of a β - tubulin and tef 1 sequence dataset (TL = 265, CI = 0.89, RI = 0.76, RC = 0.68 and HI = 0.11). MP, ML above 50 % and BPP values above 0.90 were placed close to topological nodes and separated by " / ". The bootstrap values below 50 % and BPP values below 0.90 were labelled with " - ". The tree is rooted with Diaporthe decedens (CBS 109772).

Figure 5. Diaporthe longicicola (GUCC 9146). a-b upper (a) and lower (b) surface of colony on PDA c-d conidiomata e two types of conidia f conidiophores, conidiogenous loci and conidia g α-conidia h β-conidia. Scale bars: 10 µm (e, f, g, h).