Diatrypella pseudooregonensis S.H. Long & Q.R. Li, 2021

Long, Sihan, Liu, Lili, Pi, Yinhui, Wu, Youpeng, Lin, Yan, Zhang, Xu, Long, Qingde, Kang, Yingqian, Kang, Jichuan, Wijayawardene, Nalin N., Wang, Feng, Shen, Xiangchun & Li, Qirui, 2021, New contributions to Diatrypaceae from karst areas in China, MycoKeys 83, pp. 1-37 : 1

Diatrypella pseudooregonensis S.H. Long & Q.R. Li sp. nov.

Fig. 4

Holotype.

GMB0041

Etymology.

Refers to its similar species of Diatrype oregonensis .

Description.

Saprobic on decaying branches of unidentified plant. Sexual morph: Stromata pustulate, with groups of 3-16 perithecia, rugose, visible as black, erumpent, scattered, surrounded by a thin, black line in host tissue, solitary to gregarious, 1-3 mm long and 0.5-2 mm broad (av. = 2 × 1.5 mm, n = 30), about 1 mm thick. Endostroma white to light yellow. Ostiole opening separately, papillate or apapillate, central. Perithecium immersed in stroma, globose to subglobose, glabrous, with cylindrical neck, brevicollous or longicollous 218.5-465 μm high, 112-257 μm diam. (av. = 306 × 164 μm, n = 10), globose to subglobose, glabrous, ostioles individual. Peridium: 30-50 μm thick, dark brown to hyaline with textura angularis cell layers. Asci 95-149 × 6.5-11.5 μm (av. = 120 × 10.5 μm, n = 30), 8-spored, unitunicate, clavate or cylindrical, long-stalked, apically rounded, apical rings inamyloid. Ascospores 11-16 × 1.5-3.5 μm (av. = 14 × 2.5 μm, n = 30), irregularly arranged, allantoid, slightly or moderately curved, subhyaline to slightly brown, smooth, aseptate, usually with two oil droplets. Asexual morph: undetermined.

Culture characteristics.

Ascospores germinating on PDA within 24 hours. Colonies on PDA, white when young, became pale brown, dense, but thinning towards the edge, margin rough, white from above, white at margin and light brown at centre from below, no pigmentation produced on PDA medium, no conidia observed on PDA or on OA media.

Specimens examined.

China, Yunnan Province, Baoshan City, Lancang River Nature Reserve (25°1'19.88"N, 99°35'30.68"E) on branches of an unidentified plant, 5 October 2019. Altitude: 2677 m, Y.H. Pi & Qiong Zhang, LC 323 (GMB0041, holotype, KUN-HKAS 112646, isotype, ex-type living culture GMBC0041)

Additional specimens examined.

China, Yunnan Province, Baoshan City, Lancang River Nature Reserve (25°1'13.51"N, 99°35'25.59"E) on branches of an unidentified plant, 6 October 2019. Altitude: 2630 m, Y.H. Pi & Qiong Zhang, LC 384 (GMB0043, KUN-HKAS 112681, living culture GMBC0043) ; China, Yunnan Province, Baoshan City, Lancang River Nature Reserve (25°1'15.00"N, 99°35'39.73"E) on branches of an unidentified plant, 5 October 2019. Altitude: 2698 m, Y.H. Pi & Qiong Zhang, LC 312 (GMB0040, KUN-HKAS 112674, living culture GMBC0040) ; China, Yunnan Province, Baoshan City, Lancang River Nature Reserve (25°35'19.09"N, 99°35'19.09"E) on branches of an unidentified plant, 5 October 2019. Altitude: 2569 m, Y.H. Pi & Qiong Zhang, LC 193 (GMB0039, KUN-HKAS 112667, living culture GMBC0039) ; China, Yunnan Province, Baoshan City, Lancang River Nature Reserve (25°1'9.11"N, 99°35'24.80"E) on branches of an unidentified plant, 5 October 2019. Altitude: 2649 m, Y.H. Pi & Qiong Zhang, LC 335 (GMB0042, KUN-HKAS 112647, living culture GMBC0042) ; China, Guizhou Province, Anshun City, Pingba District (26°25'9.65"N, 106°24'24.48"E) on branches of an unidentified plant, 1 August 2020. Altitude: 1250 m, Y.H.Pi, PB51 (GMB0044, KUN-HKAS 112693, living culture GMBC0044) .

Additional sequences. GMB0041 (LSU: MW797062 , RPB2: MW814906 ); GMB0043 (LSU: MW797064 , RPB2: MW814907 ); GMB0040 (LSU: MW797061 , RPB2: MW814905 ); GMB0039 (LSU: MW797059 , RPB2: MW814904 ); GMB0042 (LSU: MW797063 ); GMLB0044 (LSU: MW979054 , RPB2: MW814899 ).

Note. Morphologically, Diatrype has 8 ascospores in a single ascus, while Diatrypella has more than eight ascospores in each ascus ( Senanayake et al. 2015). However, previous research (e.g. Acero et al. 2004 and Trouillas et al. 2011) suggested that both Diatrypella and Diatrype are polyphyletic within the family. In the phylogenetic analyses, Diatrypella pseudooregonensis grouped closely to the D. verruciformis and thus, we consider this new species to belong in the genus Diatrypella , because it is doubtful whether the number of ascospores per asci is useful as a basis for generic classification.

Figures

Figure 4. Diatrypella pseudooregonensis (GMB 0041, holotype) A stromata on host substrate B, C stromata on host substrate D transverse section through ascostroma E vertical section through ascostroma F culture on PDA G section through the ascostroma H ostiolar canal I, J asci K-N ascospores. Scale bars: 20 μm (G); 10 μm (H-N).