Dothideopsella Höhn., Sber. Akad. Wiss. Wien, Math.

Ariyawansa, Hiran A., Kang, Ji-Chuan, Alias, Siti A., Chukeatirote, Ekachai & Hyde, Kevin D., 2013, Towards a natural classification of Dothideomycetes: The genera Dermatodothella, Dothideopsella, Grandigallia, Hysteropeltella and Gloeodiscus (Dothideomycetes incertae sedis), Phytotaxa 147 (2), pp. 35-47 : 38-40

Dothideopsella Höhn., Sber. Akad. Wiss. Wien, Math. -naturw. Kl., Abt. 1 124: 70. 1915. Mycobank: MB 1696

Saprobic on dead wood and stems in terrestrial habitats. Ascomata immersed, erumpent to nearly superficial, globose, subglobose or obypyriform, smooth. Ostiole well-developed, broadly or narrowly conical, with a dark brown to black papilla, without periphyses. Peridium composed of large pigmented thin-walled pseudoparenchymatic cells of textura angularis. Hamathecium composed of cellular, branched, anastomosing pseudoparaphyses, without a gelatinous coating. Asci 8-spored, fissitunicate, cylindrical to oblong, with a knob like pedicel. Ascospores fusoid or narrowly fusoid, brown or yellowish brown, septate and constricted at the septum and deeply constricted at the second septum, with smooth spore wall. Asexual state: unknown.

Type species: Dothideopsella agminalis (Sacc. & Morthier) Höhn., Sber. Akad. Wiss. Wien, Math. -naturw. Kl., Abt. 1 124: 70. 1915. FIG. 2 A–K .

MycoBank: MB 431657

≡ Leptosphaeria agminalis Sacc. & Morthier, Michelia 1(no. 5): 498. 1879.

≡ Heptameria agminalis (Sacc. & Morthier) Cooke, Grevillea 18(no. 86): 30. 1889.

≡ Phaeosphaeria agminalis (Sacc. & Morthier) Tomilin , Nov. sist. Niz. Rast. 22: 173. 1985.

Saprobic on dead wood and stems in terrestrial habitats. Ascomata 410–550× 420–580 µm (x = 465× 500 µm, n = 10), immersed, erumpent to nearly superficial, globose, subglobose or obypyriform, black to dark brown, smooth, ostiolate. Ostiole well-developed, broadly or narrowly conical, with a dark brown to black papilla, without periphyses. Peridium 50–80 µm (x = 65, n = 20) thick, composed of large pigmented thinwalled pseudoparenchymatic cells of textura angularis, thin at the upper parts and thicker and darker at the base. Hamathecium composed of dense 2–3 µm diam (x = 2, n = 20), numerous, cellular, septate, branched, hyaline, anastomosing pseudoparaphyses, without a gelatinous coating. Asci 140–185× 20–35 µm (x = 160× 25 µm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to oblong, with a knob like pedicel, apically rounded with an ocular chamber. Ascospores 30–45× 10–25 µm (x = 35× 16 µm, n = 40), sometimes uniseriate and partially overlapping, mostly biseriate, fusoid or narrowly fusoid, brown or yellowish brown, septate and constricted at the septum and deeply constricted at the second septum, with smooth spore wall. Asexual states: unknown.

Notes: Dothideopsella was introduced by Höhnel (1915) with four species and is typified by Dothideopsella agminalis (Sacc. & Morthier) Höhn. (≡ Leptosphaeria agminalis Sacc. & Morthier ), a species introduced by Saccardo (1879) The genus is placed in Leptosphaeriaceae in Index Fungorum (2013) and Dothideomycetes , genera incertae sedis in Lumbsch and Huhndorf (2010) The genus is characterized by its broad ascomatal wall, with conical, papillate ostioles, without periphyses, and multi-septate brown ascospores (Ahn and Shearer 1997). Dothideopsella shows similarities with Phaeosphaeriaceae treated in Quaedvlieg et al. 2013. Both Phaeosphaeria and Dothideopsella have immersed ascomata with short papillate ostioles, a peridium of pseudoparenchymatous cells, cellular pseudoparaphyses and pale brown, septate ascospores. Dothideopsella differs from Phaeosphaeria and other genera of Phaeosphaeriaceae in having a broader peridium and conical ostioles without periphyses. Dothideopsella is somewhat similar to Chaetoplea in having immersed ascomata, with cellular pseudoparaphyses and pale brown, septate ascospores ( Hyde et al. 2013), but differs in width of the peridium (broad versus thin), conical ostioles, without periphyses and the characters of the asci (cylindrical to oblong asci with a knob like pedicel versus cylindro-clavate asci with a thick, furcate pedicel)

Four Dothideopsella species are listed in Index Fungorum (2013) and no molecular data is available for any of these species. Therefore, fresh collections of the type species of the genus are needed so that molecular data can be obtained to verify the natural taxonomic affinities of this genus. Based on the above morphological characters we refer Dothideopsella to Phaeosphaeriaceae .

Material examined: Switzerland, Neuchâtel, on branches of Clematis vitalba L., April 1880, leg. Dr. P. Morthier, de Thümen, Mycotheca universalis, No. 1740 (ILL, holotype)

Shiraiaceae Liu YX, Liu ZY & K.D. Hyde, Phytotaxon 103: 53. 2013.

MycoBank: MB: 803214

Shiraiaceae is monotypic and typified by Shiraia bambusicola ( Liu et al. 2013) The family is characterized by superficial, pinkish ascostromata with an outer layer of external ascomata with 6-spored asci and hyaline, muriform ascospores, and central conidiomata producing similar, hyaline, muriform conidia. Phylogenetic studies based on nr LSU concluded that Shiraia bambusicola forms a robust clade within Pleosporales in the suborder Pleosporineae ( Hyde et al. 2013; Liu et al. 2013), thus a new family Shiraiaceae was introduced by Liu et al. (2013).

Figures

FIGURE 2. Dothideopsella agminalis (holotype). A. Ascomata on host substrate. B. Close up of ascomata. C. Section of ascoma. D. Close up of the peridium. E. Close up of the conical aperiphysate ostiole. F–H. Asci with short, knob-like pedicel bearing eight ascospores. I–K. Ascospores with smooth walls. Scale Bars: C = 300 µm, D = 30 µm, E = 50 µm, F–H = 80 µm, I–K = 20 µm.