Insect

Maia, Valéria Cid & Villagra, Cristian A., 2017, A new genus and species of Lasiopteridi (Diptera, Cecidomyiidae) on Haplopappus foliosus (Asteraceae) from Chile, Revista Brasileira de Entomologia 61 (2), pp. 162-169 : 163

Insect rearing and collection

Apical galls from H. foliosus ( Fig. 2 ) were collected from sites described above and were transported in paper envelopes to laboratory, where part of the sample was dissected under a microscope to obtain larvae and pupae.The remainder was kept at 50% RH, 25 ◦ C and 16/8 light/dark conditions in stand-up position with stem trunk side inserted in individual agricultural foam grow substrate (Tray PeatFOAM ®) material placed in moistened trays. The collected galls were individually kept in transparent mica polypropylene cylinders (3 cm × 5 cm) covered on top by fine mesh cotton tissue caps (50 threads/cm). Cylinders were periodically checked in order to collect any imago emerged. Additionally, live galls were isolated in the field using 5 cm × 10 cm mesh cotton tissue bags and checked every field trip for adults. Voucher specimens are deposited at the Entomological Collection of the Museu Nacional/UFRJ (MNRJ) and entomological collection of the Museo Nacional de Historia Natural, Santiago, Chile (MCCN). We identified the host plant, H. foliosus , based on morphological traits described by Klingenberg (2007).

Figures

Figs. 2–9. (2) Bud gall on Haplopappus foliosus (Asteraceae).Scale bar: 5 mm. (3–9) Haplopappusmyia gregaria Maia and Villagra,new genus and species: (3) adult: male head, frontal; (4) male scape and pedicel; (5) male 1st and 2nd flagellomeres; (6) female 1st and 2nd flagellomeres; (7) male 4th flagellomere; (8) female antenna; (9) female 5th flagellomere.