Kondoa lulangica Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 105

Kondoa lulangica Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828796. Fig. 12K, L .

Etymology: the specific epithet lulangica refers to the geographic origin of the type strain, Lulang county, Tibet.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and cylindrical, 2.4– 3.8 × 5.0– 7.6 μm and single, budding is polar ( Fig. 12K ), a sediment is formed. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is pale pink, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.6– 2.9 × 5.7– 8.6 μm ( Fig. 12L ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose (delayed), maltose, trehalose (delayed and weak), melezitose (delayed and weak), soluble starch (weak), glycerol, erythritol (delayed), D-mannitol, D-glucitol and Methyl-α- D-glucoside are assimilated as sole carbon sources. Galactose, L-sorbose, cellobiose, lactose, melibiose, raffinose, inulin, D-xylose, L-arabinose, D-arabinose, D-ribose, Lrhamnose, D-glucosamine, methanol, ethanol, ribitol, galactitol, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate and ethylamine hydrochloride are assimilated as sole nitrogen sources. Sodium nitrite, L-lysine and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 24 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Kon. lulangica differs from its closely related species Kon. changbaiensis in its inability to assimilate cellobiose, raffinose and ribitol and its ability to assimilate erythritol, Methylα- D-glucoside and grow in vitamin-free medium ( Table S1.17 ).

Typus: China, Lulang county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2004, F.-Y. Bai (holotype CGMCC 2.2762 T preserved in a metabolically inactive state, ex-type CBS 15456 = XZ36D1).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.