Kwanghwana miscanthi Karun., C.H.Kuo & K.D.Hyde, 2020

Karunarathna, Anuruddha, Phookamsak, Rungtiwa, Jayawardena, Ruvishika S., Hyde, Kevin D. & Kuo, Chang H., 2020, Kwanghwana miscanthi Karun., C. H. Kuo & K. D. Hyde, gen. et sp. nov. (Phaeosphaeriaceae, Pleosporales) on Miscanthus floridulus (Labill.) Warb. ex K. Schum. & Lauterb. (Poaceae), Cryptogamie, Mycologie 20 (6), pp. 119-132 : 124-128

Kwanghwana miscanthi Karun., C.H.Kuo & K.D.Hyde , sp. nov.

( Fig. 2 )

INDEX FUNGORUM NUMBER. — IF557101.

FACESOFFUNGI NUMBER. — FoF 07776.

ETYMOLOGY. — Refers to the generic name for the host.

HOLOTYPE. — MFLU 18-1950 .

CULTURE CHARACTERS. — Colonies on PDA slow growing at 20-25° C, circular, white at the growing edge, light to dark pink in the centre; reverse white at the edge, the centre dense, convex or dome-shaped to umbonate, with entire edge, glabrous with tiny granular on surface, no pigment produced.

MATERIAL EXAMINED. — Taiwan, Chiayi Province, Kwang Hwa, alt. 1330 m, 23°30.084’N, 120°41.73.5’ E on Miscanthus floridulus (Poaceae) leaf sheath, 17.III.2018, A. Karunarathna, AKTW 05 (holo-, MFU [ MFU 18-1952]), ex-type living culture, FU 31017, NCYUCC 19-0372.

DESCRIPTION

Associated with Miscanthus floridulus (Poaceae) leaf sheath.

Sexual state

Ascomata. 130-150 µm × 160-170 µm. (ẍ = 138 × 168 µm, n = 5), immersed or semi-immersed, visible as minute black dots on host surface, uniloculate, subglobose, brown to dark brown, solitary; centrally ostiolate, circular, papillate.

Peridium. 20-30 µm wide, with 7-9 layers at the apex and 3-4 layers at the base, thinner towards the base, the outer layers composed of brown, thick-walled cells of textura angularis, the inner layers composed of hyaline to pale brown, thin-walled cells of textura prismatica.

Hamathecium. Composed of numerous,hyaline,frequently septate, broadly cellular 2-3µm wide pseudoparaphyses,often constricted at the septa, branching at the mid-point, embedded in mucilage. Asci. 60-70 × 10-13 µm (ẍ = 67.5 × 11 µm, n = 30), 8-spored, bitunicate, cylindrical to cylindric-clavate, short pedicellate, apically rounded with well-developed narrowly ocular chamber, smooth-walled, arising from the base of ascoma.

Ascospores. 16-18.5 × 5-6 µm (ẍ = 17 × 5 µm, n = 40), fusiform, broadly fusoid with rounded ends, hyaline, overlapping or irregularly biseriate, 3-septate, the cells above and below the middle septum are broader, upper cell is comparatively broader, smooth-walled with large guttules; germinating within 12 hours on PDA, geminating from end cells.

Asexual state

Unknown.

Figures

FIG. 2.— Kwanghwana miscanthi Karun., C.H.Kuo & K.D.Hyde, sp. nov. (MFU 18-1950, holotype): A, B, appearance of ascomata on the host Miscanthus floridulus (Labill.) Warb.ex K.Schum.& Lauterb.(Poaceae) leaf sheath; C, section through ascoma; D, section through peridium at the apex; E, section through peridium at the base; F, hamathecium; G, Pseudoparaphyses; H-K, asci; L-P, ascospores; Q, germinating ascospore; R, S, culture characteristics on PDA (r = from above, s = from below). Scale bars: C, 20 µm; D-F, 25 µm; G-K, 20 µm; L-Q, 25 µm.