Lacydonia sp.

Hookabe, Natsumi, Jimi, Naoto, Tsuchida, Shinji, Fujiwara, Yoshihiro & Kajihara, Hiroshi, 2020, Fig. 21. Sesarmops mora n in Paralbunea dayriti, Zoological Studies 59 (28), pp. 1-9 : 4-6

Lacydonia sp. Tosa

Material examined: Four specimens, collected by sledging during a cruise of the oceanographic research-training vessel Toyohata-maru at a depth of 100 m about 33°18.452'N, 133°35.829'E, Tosa Bay, Kochi, off western Japan, on 24 July 2019: one specimen, preserved in formalin; another specimen, Au-coated and mounted on a SEM stub; the other two specimens used for DNA extraction.

Remarks: All the specimens were represented by anterior or posterior fragments and heavily damaged during sledging on the bottom and/or onboard sieving while extraction from bottom sediments. Detailed morphological data were not available from these specimens.

Genetic distances and phylogenetic analyses

The interspecific genetic distance based on COI was 10.4–11.3% in p -distance and 11.3–12.3% in K2P between Lacydonia japonica sp. nov. and the unidentified Lacydonia sp. Tosa ( Table 3). Our phylogenetic tree of Lacydonia shows that the two Japanese species form a clade, while the relationships among Lacydonia eliasoni , L. laureci , and another unidentified Lacydonia sp. MB-2010 were not well resolved with the sequences currently available in GenBank ( Fig. 5 ).

Figures

Fig. 5. A preliminary tree of the genus Lacydonia reconstructed with a maximum likelihood analysis based on concatenated partial sequences of COI, 16S rRNA, 18S rRNA, and 28S rRNA genes. Numbers near nodes indicate bootstrap support values (BS) generated by maximum likelihood analysis with 1,000 replicates and posterior probability (PP) of a separate partitioned Bayesian analysis (BS/PP). Eulalia viridis, Paranaitis wahlbergi, and Phyllodoce longipes were used as outgroup taxa.