Lepiota recondita Tatti, Huijser & Vizzini

Vizzini, Alfredo, Tatti, Alessia, Huijser, Henk A., Liang, Jun F. & Ercole, Enrico, 2019, Looking for Lepiotapsalion Huijser & Vellinga (Agaricales, Agaricaceae), MycoKeys 52, pp. 45-69 : 45

Lepiota recondita Tatti, Huijser & Vizzini sp. nov. Figs 7, 8, 9

Holotype.

The Netherlands, prov. Limburg, Valkenburg, Schaelsberg, 02 September 2004, Henk A. Huijser (TR gmb 01482).

Etymology.

From the Latin “reconditus”, meaning hidden, forgotten, which refers to its resemblance with L. psalion with which it was confused.

Diagnosis.

It is distinguished from Lepiota psalion by larger spores (3.7 –)4.4–5.4(– 5.9) × (2.4 –)2.9–3.6(– 4.3) μm, versiform cheilocystidia and different nrITS and nrLSU sequences.

Description.

Macrocharacters (Fig. 7). Pileus 9-26 mm wide, at first slightly obtusely campanulate, hemispherical-trapezoid or broadly conical, later plano-convex to applanate-expanded, subumbonate, with a shallow umbo; not hygrophanous; margin not striated, slightly exceeding the lamellae when young, sinuous-undulate, entire or slightly fringed with age, with minute adhering remnants of partial veil when young; surface dry, at first smooth, later irregularly cracking around centre into concentric non-uplifted squamules; pinkish-light brown at centre from [Light Pinkish Cinnamon (Plate XXIX, 15 ’’.Y-O./d) HTML f19b5f] to [Mikado brown (Plate XXIX 13 ’’.OY-O./i), HTML 9f5425] or [Sayal Brown (Plate XXIX, 15 ’’.Y-O./i) HTML bc662d], paler towards the margin: [Capucine Bluff (Plate III, 13.OY-O./f) HTML fee6cc] or [Orange Pink (Plate II, 11.ORANGE/f) HTML ecc8a3]. Stipe 26-47 × 1.5-3 mm, central, cylindrical, at first white, becoming pink-brown with manipulation [Pinkish Cinnamon (Plate XXIX, 15 ’’.Y-O./b) HTML e1934f]; minutely silky fibrillose along all length; with whitish, ascending and often incomplete annulus on the upper part of the stipe, sometimes disappearing in age; often with minute white rhizomorphs. Lamellae free, crowded, l = 1-3, at first white, soon with evident yellowish tints [Catrige Buff (Plate XXX 19 ’’.yo-y /f) HTML cdaf68] becoming [Honey Yellow (Plate XXX 19 ’’.YO-Y) HTML de9e42] when dry. Context elastic, whitish, smell weak, Lepiota cristata -like, taste not recorded. Spore-print whitish.

Microcharacters (Figs 8, 9). Spores [350, 6, 2] (3.7 –)4.4–5.4(– 5.9) × (2.4 –)2.9–3.6(– 4.3) μm, on average 4.8 × 3.3 μm, Q = (1.1 –)1.3–1.7(– 2.0), Qav = 1.5, from subglobose to oblong, mainly ellipsoid, hyaline, thin-walled, smooth, not verruculose in Melzer’s reagent, binucleate, not metachromatic in Cresyl Blue, nonamyloid, non-dextrinoid, cyanophilic in Cotton Blue (Figs 8f, 9c). Basidia mainly 4-spored, (15.8 –)17.4–25.4(– 28.6) × (5.7 –)6–7.3(– 8.8) μm (n = 60), sometimes 1-2-spored, clavate, hyaline, thin-walled (Fig. 9d); sterigmata (1.9 –)2.4–4.2(– 4.8) × (0.4 –)0.6–1.2(– 1.5) µm (n = 70). Lamella edge sterile. Cheilocystidia (20.1 –)25.4–44(– 50.0) × (3.2 –)7.2–10.4(– 12.0) µm (n = 66), numerous and crowded, hyaline, thin-walled, various in shape, mostly clavate, cylindrical-clavate, sphaeropedunculate to submoniliform, occasionally pyriform, cylindrical (Figs 8 b–d, 9b). Pleurocystidia absent. Pileus covering hymenidermic: terminal elements not tightly packed, (17 –)24.7–51.1(– 59.6) × (8.1 –)10–14(– 27.3) μm (n = 70), vesiculose, sphaeropedunculate to clavate-pyriform (Figs 8a, 9a); slightly thick-walled (walls ca 0.5 μm), with walls embedded in a thin gelatinous matrix; subpellis composed of densely arranged and branching cylindrical hyphae, (40.6 –)47.0–118.3(– 156.2) × (5.8 –)7.6–16.2(– 17.1) µm (n = 20) and containing scattered ramified oleiferous hyphae, (1.5 –)1.8–5.3(– 8.0) µm wide (n = 30). Hymenophoral trama subregular, consisting of ovate hyphae (20.9 –)21.1–40.3(– 42) × (7 –)9.6–13(– 14.5) µm (n = 12). Stipe covering and trama indistinguishable, consisting of cylindrical hyphae, (55.3-) 67.0-165.7 (-213.0) × (5.5 –)7.6–15.0(– 21.0) µm. Caulocystidia absent. Partial veil (annulus) composed of cylindrical elements, (7.2 –)22.3–59(– 70.0) × (2.0 –)2.5–4.2(– 4.7) µm (n = 20) with terminal clavate elements, (10.1 –)12.4–26.7(– 38.1) × (7.0 –)9.5–16.7(– 28.4) µm (n = 40) (Figs 8e, 9e). Clamp-connections present and abundant everywhere.

Ecology and distribution.

Gregarious on rich in nutrients and lime (marl) bare soil, in a mixed deciduous forest; so far known only from the type locality.

Collections examined.

The Netherlands, Limburg province, Valkenburg, Schaelsberg, man-made (anthropized) hilly grove with mainly deciduous trees ( Quercus , Fagus , Corylus , Fraxinus , Robinia , Prunus , Sambucus ), together with Lepiota tomentella , L. poliochloodes , Melanophyllum eyrei , and Limacella ochraceolutea , 22 September 2001, Henk A. Huijser (TR gmb 01481, paratype); ibidem, 02 September 2004, Henk A. Huijser (TR gmb 01482, holotype).