Microdochium sinense S. B. Liu, X. Y. Liu, Z. Meng & X. G. Zhang, J.

Zhang, Zhao-Xue, Shang, Yu-Xin, Zhang, Meng-Yuan, Zhang, Jin-Jia, Geng, Yun, Xia, Ji-Wen & Zhang, Xiu-Guo, 2024, Phylogenomics, taxonomy and morphological characters of the Microdochiaceae (Xylariales, Sordariomycetes), MycoKeys 106, pp. 303-325 : 303-325

Microdochium sinense S. B. Liu, X. Y. Liu, Z. Meng & X. G. Zhang, J. Fungi 2022, 8, 577.

Fig. 6

Material examined.

China, Hainan Province, Jianfengling National Forest Park, on diseased leaves of Phragmites australis , 12 April 2023, Z. X. Zhang ( HSAUP 3922-1), living culture SAUCC 3922-1; ibid., ( HSAUP 3922-3), living culture SAUCC 3922-3.

Description.

Endogenic on diseased leaves of Phragmites australis . Mycelia are superficial and immersed, 2.1–2.9 µm wide, branched, membranous and hyaline. Conidia are solitary, hyaline, straight, oblong to ellipsoid, 12.3–15 × 3.5–5.6 µm, multi-guttulate, apex rounded, base usually flattened. Conidiophores were not observed, chlamydospores were not observed, sexual morphs were not observed, see Fig. 6 .

Culture characteristics.

Cultures incubated on PDA at 25 ° C in darkness, reach-ing 72–76 mm diam., had a growth rate of 5.1–5.4 mm / day after 14 days, with moder-ate aerial mycelia, milky white to grey-white, with irregular margin, reverses light brown in the centre, with grey-white and regular margin.

Notes.

Phylogenetic analyses of four combined genes ( ITS, LSU, RPB 2 and TUB 2) showed that SAUCC 3922-1 and SAUCC 3922-3 clustered with the type collection of Microdochium sinense with strong support (Fig. 1 ). We, therefore, identified the isolated strains ( SAUCC 3922-1 and SAUCC 3922-3) as Mi. sinense . Morphologically, the conidia of the both (newly isolated and type) were similar (12.3–15 × 3.5–5.6 vs. 11.5–19.34 × 2.8–5.4 µm).

Figures

Figure 1. A maximum likelihood tree was constructed using a combined dataset of ITS, LSU, RPB 2, and TUB 2 sequence data. Branch support values, shown as ML / BIPP, are indicated above the nodes: MLBV ≥ 70 % on the left and BIPP ≥ 0.90 on the right. Ex-type cultures are denoted in bold and marked with an asterisk (*). Strains from the current study are highlighted in red. The tree was rooted with Cryptostroma corticale (CBS 218.52). The scale bar at the bottom center represents 0.05 substitutions per site.

Figure 6. Microdochium sinense a diseased symptoms on a leaf of Phragmites australis b, c colonies on PDA from above and below after 14 days d conidiomata on PDA e, f conidia. Scale bars: 10 μm (e – f).