Plenodomus sinensis Tennakoon, Phookamsak & K.D Hyde, 2017

Tennakoon, Danushka S., Phookamsak, Rungtiwa, Wanasinghe, Dhanushka N., Yang, Jun-Bo, Lumyong, Saisamorn & Hyde, Kevin D., 2017, Morphological and phylogenetic insights resolve Plenodomus sinensis (Leptosphaeriaceae) as a new species, Phytotaxa 324 (1), pp. 73-82 : 76-79

Plenodomus sinensis Tennakoon, Phookamsak & K.D Hyde , sp. nov

Index Fungorum Number: IF553195; Facesoffungi number: FoF03235, Fig.2

Etymology: — Named after the country where this fungus was collected, China.

Holotype:— MFLU17-0767

Saprobic on dead branch of Tamarindus indica L. Sexual morph: Ascomata 255–285 μm high, 375–410 μm diam., (x = 178 × 30 μm, n = 20), solitary, scattered, appearing as small, raised black dots on the host surface, superficial, globose to subglobose, smooth, easily removed from the host substrate, with a flattened base, black, ostiolate, papillate. Papilla up to 75–80 μm diam., 48–55 μm long, central circular, ostiolar canal filled with periphyses. Peridium 76–85 μm (x = 79 μm, n = 10) at sides, 24–32 μm (x = 27 μm, n = 10) at base, slightly thin at the base, composed of three layers of scleroplectenchymatous cells, inner layer comprising 2–3 cell layers of flattened, light brown cells, arranged in a textura angularis, middle layer comprising several layers of sub-hyaline cells arranged in a textura globulosa, outer layer composed of heavily pigmented, thick-walled, dark brown cells, textura angularis. Hamathecium 1.9–2.6 μm (x = 2.3 μm, n = 10) wide, septate, cellular pseudoparaphyses, branching between the asci, embedded in a gelatinous matrix. Asci (78–)80–100(–108) × (9.5–)10–12(–15) μm (x = 88× 10.8 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical, short pedicel, apically rounded, with a distinct ocular chamber. Ascospores (25–)27–40(–42) × 3.8–4.4 μm (x = 32 × 4.1 μm, n = 20), overlapping uni- or bi-seriate, olivaceous to yellowish, cylindrical to subfusoid, with obtuse ends, 6–7 septate, not or slightly constricted at each septum, cell above central septum slightly wider, guttulate, thick and smooth-walled, with mucilaginous globoid-shaped apical and basal appendages. Asexual morph: Undetermined.

Material examined – CHINA. Yunnan Province. Xishuangbanna, Nabanhe, dead branch of Tamarindus indica ( Fabaceae ), 25 November 2015, D. S. Tennakoon, DXH 015 ( MFLU 17-0767, holotype), ibid. on dead branch of Plukenetia volubilis ( Euphorbiaceae ), 26 November 2015, D. S. Tennakoon, DXH 025 ( MFLU 17-0757, paratype)

Notes − Plenodomus sinensis resembles to P. guttulatus Ariyawansa & K.D. Hyde , P. salvia Thambugala et al. and P. collinsoniae (Dearn. & House) Gruyter, Aveskamp & Verkley in having cylindrical, fissitunicate asci with short bulbous pedicels ( Ariyawansa et al. 2015). Plenodomus sinensis has an unique character in that it can be distinguished from P. guttulatus , P. salvia and P. collinsoniae in having mucilaginous globoid-shaped apical and basal appendages in ascospores(1.5–1.8 μm). As well as the cell which is above the middle septum (third cell from the apex) is slightly wider than other Plenodomus species. Furthermore, the peridium of P. sinensis consists of three types of cell layers (inner layer: light brown, textura angularis; middle layer: sub-hyaline, textura globulosa; outer layer: dark brown, thick and highly pigmented). Additionally, the host occurrence, sizes of asci, ascospores are different in each species ( Table 2).

Figures

FIGURE 2. Plenodomus sinensis (MFLU 17-0767) a Ascomata on host. b–c Vertical section of ascoma. d section through Ostiole. e Section of peridium.f Pseudoparaphyses. g–jAsci. k–o Ascospores. p–q Mucilaginous globoid-shaped apical and basal appendages.Scale bars: b–c = 50 μm, d–j = 20 μm, k– o = 5 μm.