Polypedilum (Pentapedilum) nubens (Edwards)

Oyewo, Emmanuel Adeoye & Saether, Ole A., 2008, Revision of Polypedilum (Pentapedilum) Kieffer and Ainuyusurika Sasa et Shirasaki (Diptera: Chironomidae), Zootaxa 1953, pp. 1-145 : 82-85

Polypedilum (Pentapedilum) nubens (Edwards)

( Figs 26–27)

Pentapedilum nubens Edwards, 1929: 376 ; Pinder (1978: 134).

Polypedilum (Pentapedilum) nubens (Edwards) ; Ashe and Cranston (1990: 301).

Material examined. TURKEY: West Anatolia, Koruköy , 1 male, 30.v.1992, H. Malicky ( ZSM No. TR 29) . ITALIA: Basilicata, Trecchina , 1 male, 02.ix.94, A. Hausmann ( ZSM) . NORWAY: Hordaland, Bergen , 2 males, 27.v.1990, Ø. A. Schnell & E. Willassen ( ZMBN) . AFGHANISTAN: (locality not stated), K. Lindberg ( ZSM No. A485) . GERMANY: Bodensee, Bodanrück , 3 pupae, 12.vi.1962, F. Reiss ( ZSM No. I); Bodensee bei Bodman, 3 pupae, 12.vi.1962, F. Reiss ( ZSM No. I) . ITALY: Lago Maggiore , 3 pupae, 9.v.1960, F. Lenz ( ZSM) . TUNISIA: Gouvernorat de Tunis, Saint-Germain , 1 male, 12.ix.1942, Clastrier ( ZSM No. 520) . MOROCCO: South Marrakesh, Lalla Taerkoust dam, 1 pupa, 26.vi.1992, H. Laville ( ZSM) .

Diagnostic characters. Among species with numerous setae on the wing membrane, uniformly colored abdomen, foretibial scale without spur, base of superior volsella with microtrichia, tergite IX with less than 15 median setae, and cell m basal of RM with more than 10 setae, the species is characterized by having a slightly spatulate anal point, cell m basal of RM with 22–33 setae, squama with 7–15 setae, and projection of superior volsella normally without a lateral seta. The species is close to P. (Pe.) uncinatum , which, however, has a longer, parallel-sided anal point and always has a lateral seta on the projection of the superior volsella. The pupa can be distinguished from other Pentapedilum species by the low number of taeniae (10–13), caudolateral spur with 1 apical or bifid tooth and 1–3 lateral teeth plus several accessory points. Conjunctive III / IV lack spines, and pedes spurii B is not well developed.

Male (n = 5–6, unless otherwise stated)

Total length 3.63–3.74 (3) mm. Wing length 2.05–2.31 (3) mm. Total length / wing length 1.58–1.78 (3). Wing length / length of profemur 2.41–2.73 (3). Thorax and abdominal segments dark brown to brown; femur, tibia and tarsi pale brown.

Head ( Fig. 26A). AR 1.21–1.56, 1.45. Ultimate flagellomere 517–685, 609 µm long. Temporal setae 12 including 2–5, 4 inner verticals; 3–5, 4 outer verticals; and 2–3, 3 postorbitals. Clypeus with 16–24, 19 setae. Tentorium 117–143, 131 µm long; 5–9, 7 µm wide at posterior tentorial pit. Stipes 131–162, 148 µm long. Palpomere lengths (in µm): 26–47, 36; 39–58, 49; 135–159, 144; 136–148, 140; 200–221, 211. Fifth palpomere / third palpomere 1.25–1.57, 1.46. Third palpomere with 2 sensilla clavata.

Thorax ( Fig. 26B). Acrostichals 10–12, 11; dorsocentrals 14–24, 17; prealars 4–6, 5 (4). Scutellum with 7–8, 8 (5) setae.

Wing ( Fig. 26E). Anal lobe developed. VR 1.09–1.21, 1.15. Brachiolum 2–3, 3 setae; R with 31–61, 48;

R 1 with 37–43, 40; R 4+5 with 57–88, 72; Sc with 14–33, 22; RM with 0–2, 1; M with 8–12, 10; M 1+2 with 56– 67, 61; M 3+4 with 54–65, 59; Cu with 29–40, 32; Cu 1 with 19–32, 24; PCu with 28–44, 40; An with 10–34, 18 setae. Cells r 4+5, m 1+2 and m 3+4 each with more than 100 macrotrichia; cell m basal of RM with 22–33, 30; cu plus an with 90 to more than 100 macrotrichia. Squama with 7–15, 11 (4) setae.

Legs. Scale on foretibia ( Fig. 26C) 29–37, 34 µm long, triangular, without apical spur. Spurs on midtibia

( Fig. 26D) 48–51, 46 µm long; on hind tibia 57–64, 58 µm long. Comb on midtibia 12–23, 17 µm long; combs on hind tibia 14–28, 20 µm and 12–21, 13 µm long. Width at apex of foretibia 38–49, 45 µm; of midtibia 28–49, 36 µm; of hind tibia 55–64, 58 µm. Length and proportions of legs as in Table 31.

Hypopygium ( Fig. 26F). Tergite IX with 7–13, 10 median setae, laterosternite IX with 2–3, 3 setae. Anal point 51–64, 57 µm long, 6–14, 10 µm wide at base, gradually tapering to nearly parallel-sided towards 5–10, 8 µm wide apex. Transverse sternapodeme 26–65, 42 µm long; phallapodeme 58–100, 78 µm long. Gonocoxite 129–193, 148 µm long. Superior volsella ( Fig. 26H–K) 58–81, 72 µm long; base bare with 1–5, 3 inner setae; projection rarely with lateral seta. Inferior volsella ( Fig. 26G) 93–125, 108 µm long, nearly parallelsided, with prominent apical seta. Gonostylus 145–179, 165 µm long. HR 0.75–1.13; HV 1.63–2.23 (3).

wise stated).

Pupa (n = 10, unless otherwise stated)

Total length 3.31–4.88, 4.09 (6) mm. Frontal setae, exuviae cephalothorax, margins of segments and caudolateral spur brown.

Cephalothorax ( Figs 27A–C). Frontal apotome as in Figure 27A, cephalic tubercules absent. Frontal setae 36–57, 53 (5) µm long. Prealar tubercle apparently absent. Wing sheath 1.17–1.54, 1.40 mm long.

Abdomen ( Figs 27D–I). Tergite I bare; tergites II – VI each with strong, prominent anterior row of spines. Tergites II – VI with medial anterior to posterior patches of shagreen, VII with sparse, weak anterior to posterior shagreen. Tergites VII and VIII both bare or with paired anterolateral spots of fine spinules, and without shagreen. Conjunctive IV / V with 2–3 transverse bands of posterior pointed spinules or spines. Tergite II with single row of 53–89, 71 (6) caudal hooks. Pedes spurii A present on segment IV. Pedes spurii B weakly developed on II. Caudolateral spur ( Fig. 27I) 49–98, 82 µm long, with bifid tooth or occasionally 1 strong apical tooth with several accessory points. Segments I– IV without L taeniae, V and VI each with 3 taeniae; VII and VIII each with 4 taeniae. Anal lobe fringe with 10–13, 12 (8) taeniae. Length of genital sac overreach 191– 416, 301 µm.

Remarks. The foretibial scale is triangular with blunt apex. One of two specimens from Bergen, Norway, has a lateral seta on the projection of the superior volsella, absent in all other examined specimens. All specimens observed lack microtrichia on the superior volsella, although specimens described and illustrated by some authors (e.g. Pinder 1978) bear microtrichia.

Distribution. Known from the Czech Republic, France (including Corsica), Germany, Great Britain, Ireland, Italy, Macedonia, Norway, Portugal, Spain, North Africa, Iran, Switzerland, Iraq, Turkey, Russia, Georgia, Armenia, Azerbaijan, Lebanon, Syria, Israel, Jordan ( Saether & Spies 2004).