Pseudosterigmatospora motuoensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 111-113

Pseudosterigmatospora motuoensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB832545. Figs 11D and 14P .

Etymology: the specific epithet motuoensis refers geographic origin of the type strain, Motuo, Tibet.

to the

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 2.2–3.0 × 3.7– 5.3 μm and single, budding is polar ( Fig. 14P ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. One or more conidia are produced on each stalk-like conidiophore. Conidiophore is single or multiple, usually bifurcate, somewhat trifurcate. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed and weak), L-sorbose (delayed and weak), sucrose, maltose (delayed and weak), trehalose, melezitose, ethanol, Dmannitol, D-glucitol and salicin (delayed and weak) are assimilated as sole carbon sources. Cellobiose, lactose, melibiose, raffinose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, glycerol, erythritol, ribitol, galactitol, Methyl-α- D-glucoside, D-gluconate, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite (delayed and weak), L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride (delayed and weak) are assimilated as sole nitrogen sources. Maximum growth temperature is 26 – 27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Typus: China, Motuo, Tibet, obtained from a leaf of Achyrospermum wallichianum, Sep. 2014 , Q.-M. Wang (holotype CGMCC 2.5816 T preserved in a metabolically inactive state, ex-type CBS 15591 = XZ119B3).

Figures

Fig. 11. SEM image of vegetative cells grown in YM broth for 5 d at 17 °C. (A) Fi. mali CGMCC 2.4012T, Bars = 4 μm; (B) Boe. sterigmata CGMCC 2.4539T, Bars = 5 μm; (C) St. layueensis CGMCC 2.5817T, Bars = 5 μm; (D) Pse. motuoensis CGMCC 2.5816T, Bars = 2 μm; (E) Me. layueensis CGMCC 2.5818T, Bars = 5 μm; (F) Beg. foliicola CGMCC 2.3164T, Bars = 1 μm; (G, H) Ros. petaloides CGMCC 2.3446T, G Bars = 10 μm, H Bars = 3 μm.

Fig. 14. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Kon. rhododendri CGMCC 2.3463T; (C, D) Ben. wuzhishanensis CGMCC 2.3569T; (E) Ben. pseudorectispora CGMCC 2.5677T; (F) Ps. fusiformis CGMCC 2.5823T; (G, H) Ru. fanjingshanensis CGMCC 2.4542T; (I, J) Ru. bangxiensis CGMCC 2.3454T; (K, L) Ru. lunata CGMCC 2.4426T; (M, N) Boe. sterigmata CGMCC 2.4539T, (O) St. layueensis CGMCC 2.5817T; (P) Pse. motuoensis CGMCC 2.5816T. Bars = 10 μm.