Ramularia titarpaniensis Kushwaha & Raghv. Singh, 2020

Kushwaha, Prakash, Singh, Raghvendra & Chaurasia, Balmukund, 2020, Ramularia titarpaniensis-a new species of ramularioid complex from central India, Phytotaxa 429 (4), pp. 274-280 : 275

Ramularia titarpaniensis Kushwaha & Raghv. Singh sp. nov. ( Figs. 1–4 )

MycoBank: MB823497

Diagnosis:— Longer and thicker conidia with more septa, when compared to other ramularioid fungi on host family Rubiaceae .

Etymology:— derived from Titarpani village, the name of place of collection.

Type:— INDIA. Madhya Pradesh: Sagar, Deori, Titarpani village, 23.3898° N, 79.0165° E, on living leaves of Wendlandia sp. ( Rubiaceae ), Jan. 2016, leg. Prakash Kushwaha, AMH 9826 (holotype), MH-DHSGU 240 (isotype).

Leaf spots amphigenous, subcircular to irregular, start at tip and spread towards base of leaves, distinct, dark brown to blackish, 1–4 mm diam. Colonies amphiphyllous, mostly hypophyllous, effuse. Mycelium internal. Hyphae branched, septate, hyaline, smooth, thin-walled. Stromata present, well developed, compact, pseudoparenchymatous, 20–50 × 20–60 µm. Conidiophores macronematous, fasciculate, erect to slightly curved, unbranched, hyaline, smooth, 1–2-septate, thin-walled, 26–63 × 3–6 µm. Conidiogenous cells integrated, terminal, cylindrical, with thickened conidial scars, 2–3.5 µm wide. Conidia hyaline, solitary, rarely catenate, straight to curved, cylindrical, mostly 1–3- septate but rarely up to 11-septate, mostly smooth, rarely verruculose, thin-walled, 10–114 × 3–9 µm, hilum thickened, 2–3.5 µm wide.

Figures

FIGURE 1. Ramularia titarpaniensis on Wendlandia sp. a, b. Infected host plant. c. Infection spots on upper surface. d. Infection spots on lower surface. Scale bars c–d = 10 mm.

FIGURE 2. Microphotographs of Ramularia titarpaniensis (holotype, AMH 9826). a–c. Conidiophores in fasicle with stromata. d. Developing conidia at conidiogenous loci. Scale bars a, c = 20 µm, b = 30 µm, d = 15 µm (arrows indicate developing conidia at conidiogenous loci).

FIGURE 3. Microphotographs of Ramularia titarpaniensis (holotype, AMH 9826). a–d. Conidia (arrows indicate catenation points). e, f. Germinating conidia. Scale bars a = 10 µm, b, c = 25 µm, d–f = 20 µm.

FIGURE 4. SEM of Ramularia titarpaniensis (holotype,AMH 9826).a. Catenation in conidia.b, c. Conidia. d. Top view of conidiogenous cell. e, f. Conidiogenous loci. g–i. Hila of conidia. Scale bars a = 10 µm, b = 5 µm, c = 3 µm, d = 2 µm, e–i = 1 µm.