Russula multilamellula B. Chen & J. F. Liang, 2021

Chen, Bin, Song, Jie, Zhang, Jin-Hua & Liang, Jun-Feng, 2021, Morphology and molecular phylogeny reveal two new species in Russula sect. Ingratae from China, Phytotaxa 525 (2), pp. 109-123 : 117

Russula multilamellula B. Chen & J. F. Liang , sp. nov. ( Figs 2E–H , 5,6)

MycoBank: MB 836307

Etymology: — multilamellula (Latin) , referring to the species has many lamellulae.

Holotype:— CHINA. Guizhou Province, Zunyi city, Daozhen County, Yuxi Town , 24 September 2014, on the ground in Pinus forest, 28°53′26.22″N, 107°36′31.05″E, 819m asl., leg. LHJ14092423 ( RITF 3346 ).

Diagnosis: — Basidiomata medium–sized; brownish orange to hazel centre, margin satin white to yellowish-white with brownish tinge; lamellulae often present, usually irregular in length and often anastomosing with lamellae; lily white stipe with brownish spot at the base, slightly curved towards centre; subglobose to broadly ellipsoid spores with short or long ridges; hymenial cystidia apically mainly obtuse, contents granulose or crystalline, turning to yellowishbrown in SV; marginal cells sometimes flexuous; Hyphal terminations near the pileus margin sometimes flexuous, terminal cells apically usually obtuse, occasionally attenuated; pileocystidia always one-celled, apically often obtuse, contents turning light purple in SV.

Description: — Basidiomata medium-sized, 50–75 mm in diam., hemispheric to planoconvex with a slightly depressed centre above the stipe; margin incurved, tuberculate striation up to the 1/3 of the radius; cuticle smooth, glabrous, slightly viscid when wet, peeling readily; brownish orange (6C8) to hazel (6E7) at the centre, margin satin white (2A1) to yellowish-white (3A2) with brownish tinge. Lamellae adnate to adnexed, relatively dense, up to 6mm deep, lily white(1A1) to satin white (2A1); lamellulae often present, (0)1–3 between each pair of the lamellae, usually irregular in length and often anastomosing with lamellae; furcations absent or rare; edge entire and concolour. Stipe 50–60 × 12–16 mm, central to subcentral, subcylindrical to cylindrical, slightly curved towards centre, slightly inflate near the base, rugulose longitudinally, surface dry, lily white (1A1) with brownish spot at the base, pithy and hollow. Context 2–3 mm thick at pileus centre, white to cream, without colour changing when bruised; tastes lightly bitter; odour indistinct. Spore print white to cream.

Spores (5.6–)6.3–6.9–7.4(–8) × (5–)5.8–6.4–7(–7.3) μm, Q=(1.0–)1.01–1.07–1.13(–1.25), subglobose to broadly ellipsoid; ornamentation of large, moderately distant to dense (4–7 in a 3 μm diam. circle) amyloid warts, up to 1.5 μm high, occasionally fused in long chains (0–2 fusions in the circle), often forming short or long ridges (2–3 fusions in the circle), line connections absent; suprahilar spot large, inamyloid. Basidia (38.8–)45.3–52.9–60.4(–85) × (5.6–)10.8– 13.7–16.6(–18.2) μm, mostly 4-spored, some 2- and 3-spored, subclavate or clavate; basidiola subcylindrical or clavate, ca. 7–15 μm wide. Hymenial cystidia on lamellae sides moderately numerous, ca. 1000/mm 2, (61.6–)73.5–84.5–95.5(– 115) × (6.9–)10.2–12.6–15(–18.8) μm, clavate or subcylindrical, apically obtuse and mucronate, often with 2–6 μm long appendage, thin-walled, contens granulose or crystalline, turning to yellowish-brown in SV. Hymenial cystidia on lamellae edges (43–)54.7–67–79.3(–88.5) × (5–)8.6–11.3–14(–20) μm, often smaller, clavate or fusiform, apically mainly obtuse, occasionally mucronate, sometimes with 2–5 μm long appendage, contents granulose or crystalline, thin-walled, turning to yellowish-brown in SV. Marginal cells (10–)12.3–16.7–21(–28) × (3–)4.4–5.4–6.5(–8) μm, narrowly clavate or fusiform, often flexuous. Pileipellis orthochromatic in Cresyl blue, not sharply delimited from the underlying context, 180–260 μm deep, two-layered, weakly gelatinized; suprapellis 120–160 μm deep, consisting of erect or repent hyphal terminations; subpellis 80–120 μm deep, consisting of horizontally orientated, relatively dense, intricate, 2-6 μm wide hyphae. Hyphal terminations near the pileus margin occasionally branched, sometimes flexuous, thin-walled; terminal cells (12.9–)17.3–24.6–32(–40.3) × (3–)3.5–4–4.7(–5.6) μm, mainly cylindrical, rarely lageniform or fusiform, apically usually obtuse but occasionally attenuated; subterminal cells often shorter and wider, ca. 3–7 μm wide, unbranched or occasionally branched. Hyphal terminations near the pileus centre less flexuous; shorter terminal cells (8.4–)9.5–13.6–17.6(–23.6) × (2.4–)3.4–4.3–5.3(–7.4) μm, mainly cylindrical and clavate, apically usually obtuse and rarely attenuated; subterminal cells usually wider, ca. 3–6 μm wide, unbranched or rarely branched. Pileocystidia near the pileus margin always one–celled, (40.7–)45.3–59.3–73.3(–90) × (3.6–)4.2–5–6(–7) μm, narrowly clavate to subcylindrical, often flexuous, apically usually obtuse, with granulose contents, turning light purple in SV. Pileocystidia near the pileus centre often longer and wider, (53.5–)61.5–77.4–93.4(–120) × (4.8–)5.6– 9.2–12.9(–18.8) μm, usually clavate or subcylindrical, often flexuous, apically obtuse and with similar contents, turning light purple in SV. Cystidioid hyphae in subpellis and context, with granulose contents. Oleiferous hyphae abundant in subpellis, with oil contents.

Additional specimens examined: — CHINA, Guizhou Province, Tongren city, Mount Fanjing National Nature Reserve , 11 July 2012, 27°56′36″N; 108°36′47″E, 810m, asl., leg. Wuxingliang 03 ( RITF3260 ) .

Figures

FIGURE 2. Russula clavulus (RITF 3334, Holotype).A–B Basidiomata, Scale bars= 20mm.C–D Spores in SEM (JSM-6510LV); Russula multilamellula (RITF 3346, Holotype). E–F Basidiomata, Scale bars = 15 mm. G–H Spores in SEM (JSM-6510LV).