Sergey tzotzil Martinez & Zaldivar-Riveron

Martinez, Juan Jose, Lazaro, Rubi Nelsi Meza, Pedraza-Lara, Carlos & Zaldivar-Riveron, Alejandro, 2016, Sergey gen. n., a new doryctine genus from temperate forests of Mexico and Cuba (Hymenoptera, Braconidae), ZooKeys 589, pp. 143-164 : 156-159

Taxon classification Animalia Hymenoptera Braconidae

Sergey tzotzil Martinez & Zaldivar-Riveron sp. n. Figs 30-35, 36-37

Diagnosis.

See diagnosis of Sergey tzeltal .

Description.

Body length 3.7mm (Fig. 30), fore wing 3.2mm; ovipositor sheaths 2.3mm. Colour: head uniformly brown, antenna brown, gradually darkening towards apex, except for a light band composed of most of the 19th and the basal half of the 20th flagellomeres (Fig. 34). Mesosoma uniformly dark brown, except for a slightly lighter area on median area of mesoscutum. Metasoma brown. Legs light brown, except fore and middle coxae, trochanters and trochantelli and hind trochantellus which are pale yellow; hind coxa and apical three fourths of hind femur dark brown. Wings hyaline; pterostigma and veins brown. Ovipositor sheaths brown.

Head: in anterior view 0.9 times as high as wide (Fig. 31), and 0.6 times as long as wide in dorsal view (Fig. 32). Clypeus, face, frons and vertex smooth and shining; temple smooth (Fig. 33). Eye 1.3 times higher than wide. Malar space height/eye height ratio 0.4. Temple/eye length ratio (dorsal view) 0.6. Antenna with 25 flagellomeres, first flagellomere five times longer than wide and about as long as the second one.

Mesosoma: 2.1 times longer than wide (Fig. 35) and 2.0 times longer than high (Fig. 36). Pronotal groove wide, deep, and scrobiculate, pronotal carina distinct. Propleuron smooth. Mesoscutum slightly transverse, 0.7 times as long as wide. Mesoscutal lobes smooth, notauli deep and scrobiculate, obscured in an irregularly rugose median area before reaching the scuto-scutellar suture. Prescutellar sulcus with three distinct carinae. Scutellar disc smooth and triangular. Mesopleuron smooth. Precoxal sulcus, deep and scrobiculate, running along basal two-thirds of mesopleuron. Subalar sulcus deep and scrobiculate-rugose. Metanotum with a distinct median projection. Metapleuron entirely areolate rugose. Propodeum with two divergent carinae running fron median anterior edge delimiting two dorsolateral areas, these areas are mostly smooth, but turn rugose areolate near carinae; beyond these carinae the propodeum almost uniformly areolate-rugose.

Wings: Fore wing length 2.9 mm, length/width ratio 3.3; vein 1cu-a slightly postfurcal to vein 1M; veins 2RS/2M ratio 0.5.

Legs: Fore tibia with a row of spines. Hind coxa transversally striate dorsally, smooth ventrally, with a distinct basoventral tubercle.

Metasoma: Basal sternal plate/length of first tergum 0.6. First metasomal tergite 2.1 times longer than apically wide (Fig. 37). Second median tergite longitudinally costate on basal three fourths, smooth apically. Suture between second and third median tergites almost straight. Remaining terga smooth and polished. Ovipositor length 2.4 mm, 1.1 times as long as metasoma.

Males. Similar to female, slightly smaller and with antenna uniformly brown.

Distribution.

Known only from the type locality in El Triunfo, Chiapas, Mexico.

Biology.

Unknown.

Comments.

This species and Sergey tzeltal were collected in the same locality in Chiapas.

Etymology.

The name of this species refers to the Tzotzil ethnic group, descendant from the Mayans, who inhabits the Altos, a mountain region located in central Chiapas.

Material examined.

Holotype (CNIN): female, Mexico, Chiapas, Mpo. Albino Corzo, Reserva el Triunfo, 15° 39.428N, 92° 48.67W, YPT, 16/XI/2001, Kovarik col., DNA voucher number CNIN717, GenBank accession nos. KC821999 (COI), KC822259 (cyt b), KX074199 (28S), KC822124 (wingless, not included in this work), KC822080 (EF-1alpha; not included in this work). Paratype (CNIN): one male, same data as holotype; DNA voucher number, CNIN716, DNA voucher nos. KC821998 (COI), KC822258 (cyt b), KX074198 (28S), KC822123 (wingless, not included in this work), KC822079 (EF-1alpha; not included in this work).

Gene genealogies.

Intraspecific corrected genetic divergences varied from 0 to 2.1 (excluding CNIN573), 0.27 to 3.18 and 0 to 0.33% for COI, cyt b and 28S, respectively. Interspecific distances within Sergey on the other hand ranged from 7.99 to 15.28, 12.64 to 13.6 and 0.17 to 0.5% for COI, cyt b and 28S, respectively.

The Bayesian phylograms derived from the separate COI and cyt b analyses are included in the Figure 38. The COI bayesian phylogram significantly supported the monophyly of the three described species. Sergey coahuilensis was recovered as sister to Heterospilus but without statistical support (PP = 0.5). A clade with the remaining species of Sergey (PP = 0.5) recovered Sergey cubaensis from Cuba (PP = 0.5) as sister to a Sergey tzotzil (Chiapas) + Sergey tzeltal (Chiapas and Oaxaca) clade (PP = 1.0). Within Sergey tzeltal , there were three non-significantly supported, subclades, two of which were composed of specimens from Comaltepec, Oaxaca, but with one of them being more closely related to the subclade containing the specimens from El Triunfo, Chiapas.

The bayesian phylogram derived from the cyt b sequences yielded similar relationships with the COI topology. Again, some of the specimens of Sergey tzeltal from Comaltepec, Oaxaca were more closely related to the ones from El Triunfo, Chiapas (PP = 0.6) than with the remaining specimens from the same locality. The 28S tree was largely unresolved (phylogram not shown), with the sequenced specimens Sergey tzeltal and Sergey tzotzil grouped together (PP = 1.0). The reconstructed mt gene genealogies, together with the geographic provenance and morphological variation found in the specimens of Sergey tzeltal from Oaxaca and Chiapas suggests that this taxon could consist of two sympatric, recently derived lineages in which there is incomplete lineage sorting or hybridization. Further morphological and genetic studies will help to confirm the taxonomic status of the populations of Sergey tzeltal from the latter two Mexican regions.