Terfezia lusitanica Bordallo, Ant. Rodr., Louro, Santos-Silva, Muñoz-Mohedano, 2018

Bordallo, Juan-Julián, Rodríguez, Antonio, Santos-Silva, Celeste, Louro, Rogério, Muñoz-Mohedano, Justo & Morte, Asunción, 2018, Terfezia lusitanica, a new mycorrhizal species associated to Tuberaria guttata (Cistaceae), Phytotaxa 357 (2), pp. 141-147 : 144-145

publication ID	https://doi.org/ 10.11646/phytotaxa.357.2.7
persistent identifier	https://treatment.plazi.org/id/03A70044-FFB3-FF95-FF24-ABA2FA5FFBA7
treatment provided by	Felipe
scientific name	Terfezia lusitanica Bordallo, Ant. Rodr., Louro, Santos-Silva, Muñoz-Mohedano
status	sp. nov.

Treatment

Terfezia lusitanica Bordallo, Ant. Rodr., Louro, Santos-Silva, Muñoz-Mohedano View in CoL sp. nov. Fig. 2 View FIGURE 2 .

MycoBank MB 824055

Type:— Spain, Extremadura, Valdehúncar, 29 April 2016, leg Ant. Rod. (Holotype, MUB Fung-j682, GenBank accession MG 818753).

Diagnosis: — Ascomata hypogeous to partially emergent at maturity, subglobose to ellipsoid or partially flattened, sometimes with tapered sterile base, 2–3.5 cm in size, light ochre colour at first, becoming yellowish brown with black spots, smooth to slightly rough ( Fig. 2 a–c View FIGURE 2 ). Peridium 200–500 μm thick, not separable from gleba, poorly delimited, whitish in cross section, pseudoparenchymatous, composed of subglobose cells, hyaline and thin-walled in the innermost layers, yellowish and with thicker walls in the outermost layers ( Fig. 2d View FIGURE 2 ). Gleba solid, fleshy, succulent, whitish at first ( Fig. 2c View FIGURE 2 ), darkening with age, becoming greenish black at maturity; with blackish grey pockets of fertile tissue surrounded by whitish, sterile, veins ( Fig. 2b View FIGURE 2 ). Faint odour, not distinctive. Mild taste. Asci nonamyloid, subglobose to ellipsoid, sessile, 60–80 x 50–65 μm, walls 1 μm thick, with 6–8 irregularly disposed spores, randomly arranged in the gleba. Ascospores globose, (20–)21–23(–24) μm diam (median = 22 μm) including ornamentation; (14–)15–17(–18) μm (median = 16 μm) without ornamentation; hyaline, smooth and uniguttulate at first, by maturity dark yellow to light brown and ornamented with conical, blunt spines, sometimes cylindrical, mostly straight, but sometimes curved, separated, 3–3.5(–4) μm long, 1–2 μm wide at the base ( Fig. 2e–f View FIGURE 2 ).

Ecology/Distribution:— Extremadura ( Spain) and Alentejo ( Portugal), in sandy, acid soils, in grassland areas without trees, associated exclusively with Tuberaria guttata , in April.

Etymology:—Referring to Lusitania, the name gave by the Romans to the western region of the Iberian Peninsula, which now covers the Portuguese area below Douro river and the neighbouring regions of Spanish Extremadura.

Additional collections examined:— PORTUGAL: Alentejo, Montemor-o-Novo, 03 April 2012, C. Santos-Silva (MUB Fung-j469, UEVH-FUNGI 2003065, MG818752). Same locality, 20 April 2017, C. Santos-Silva (MUB Fungj817, UEVH-FUNGI 2003876, MG818754).

Notes:— T. lusitanica differs morphologically from other spiny-spored Terfezia , that share the same habitat, by the combination of its ochre peridium colour and spores size, and from all other Terfezia in its ITS nrDNA sequence. For instance, T. fanfani differs from T. lusitanica showing a reddish peridium; T. extremadurensis has ochre peridium, but exhibits distinctly larger spores than T. lusitanica ; T. extremadurensis presents a tuber-like gleba, with meandering veins not completely surrounding the fertile tissue, and does not form pockets ; T. cistophila , has smaller spores than T. lusitanica , possesses a distinctive spermatic odour, and different host plants, never found associated with T. guttata .