Trichoglossum septatum Ekanayaka, Q. Zhao & K.D. Hyde, 2017

Ekanayaka, Anusha H., Hyde, Kevin D., Jones, E. B. Gareth, Zhao, Qi, Elgorban, Abdallah M. & Bahkali, Ali H., 2017, A new species of Trichoglossum (Geoglossales, Ascomycota) from Thailand, Phytotaxa 316 (2), pp. 161-170 : 163-164

Trichoglossum septatum Ekanayaka, Q. Zhao & K.D. Hyde , sp. nov. ( Fig. 2 )

Index Fungorum number: IF552945 Facesoffungi number: FoF 03236

Etymology:—The specific epithet septatum refers to the ascospore septation.

Holotype:— Thailand, Chiang Mai Province, on soil, November 18, 2016, H. Maoqiang HD058 ( MFLU 17– 0283 ).

Diagnosis:—Apothecia club-shaped, black. Hymenium composed of blackish-brown, thick hymenial setae. Asci long, amyloid, 8-spored. Ascospores long, 7 or 15-septate.

Description:— Saprobic on soil. Sexual morph: Apothecia 2–4 mm diam. × 18–35 mm high, scattered, stipitate, slender, clavate, oval or dumbbell-shaped, hirsute, black, ascogenous portion and sterile portion are clearly distinguishable. Ascogenous portion flattened clavate to lanceolate, sometimes curved, black, densely hirsute with setae. Sterile portion cylindrical or flexuous, densely hirsute from setae, concolorous with fertile part. Ascomatal core composed of brownish cells of textura epidermoidea. Hymenial setae 400–600 × 15–25 μm (x = 520 × 21 μm, n=20), blackish-brown, acuminate, tapered at the apex and base, thick-walled, septate, sometimes protruding above hymenium. Paraphyses 7–9 μm (x = 8.2 μm, n=20) wide at the apices, numerous, filiform, septate, apically enlarged and curved, light brown at the base and darker at the apex, walls granulate. Asci 300–410 × 25–40 μm (x = 341 × 32 μm, n=20), 8-spored, cylindrical-clavate, narrowed below, apex rounded, amyloid, arising from croziers. Ascospores 190–270 × 7–9 μm (x = 230 × 8.5 μm, n=30), light brown when immature and greenish-brown to dark brown at maturity, fusoid to fusoid-clavate, immature spores aseptate, mature spores 7 or 15-septate, single ascus can have spores with both variations in the number of septation, guttulate. Asexual morph: Unknown.

Material examined:— THAILAND. Chiang Mai Province, Mae-Tang district, (Mushroom research center) MRC, on soil, 18 November 2016, H. Maoqiang HD058 (MFLU 17–0283).

Notes:— Trichoglossum septatum is characterized by having larger asci and ascospores and immature ascospores that are light brown and aseptate, while the mature ascospores are dark brown and 7 or 15-septate ( Table 2). Trichoglossum septatum is morphologically and phylogenetically close to T. hirsutum . However, they differ in asci and ascospore sizes ( Table 2).

Figures

FIGURE 2. Trichoglossum septatum MFLU 17-0283 (holotype). a. Apothecia, b. Close up of apothecial surface showing extended hymenial setae, c. Close up of hymenium, d. Hyphal cells at the ascomatal core, e. Dark brown hymenial setae, f. Septate, unbranched paraphyses, g–i.Cylindrical asci in water,j. Cylindrical asci inMelzer’s reagent withJ+, apical apex,k–p.Elongate-subfusiform ascospores. Scale bars b=500 μm, c=200 μm, d=25 μm, e=150 μm, f=100 μm, g–j=100 μm, k–m=50 μm, n–p=60 μm.