Tylopilus pallidocervicolor Z.J. Lin & L.H. Qiu, 2023

Lin, Zhi-Jun, Li, Hui-Bin, Liu, Xiao-Chun, Liang, Ying-Shan, Huang, Xiao-Xia & Qiu, Li-Hong, 2023, Tylopilus pallidocervicolor, a new species from Baiyun Mountain, Guangzhou, China, Phytotaxa 619 (2), pp. 137-151 : 139-144

Tylopilus pallidocervicolor Z.J. Lin & L.H. Qiu , sp. nov. ( Figs. 3 , 4 )

MycoBank: MB 848015

Etymology:—“ pallido ” (Lat.)—pale; “ cervicolor ” (Lat.)—deer color, referring to the color of the basidiomata.

Holotype:— CHINA, GUANGDONG PROVINCE: Guangzhou City, Baiyun Mountain , 23°10′10″ N 113°17′31″ E, elev. 120 m, 20 August 2022, L.H. Qiu, GDGM91094 (GenBank acc. no.: OQ617298 for nrITS, OQ617304 for nrLSU, OQ676968 for rpb1, OQ676973 for rpb2, OQ676977 for tef1-α).

Diagnosis:— Tylopilus pallidocervicolor is characterized by tannish brown to light brown basidiomata, stipe with distinct reticulum on upper portion, and a palisadoderm pileipellis.

Description:— Basidiomata medium to large-sized. Pileus (3.5) 5–11 cm in diam., hemispherical to subhemispherical when young, applanate to plano-depressed at maturity; surface dry, finely tomentose, reddish brown (#8C3613) to dark brown (#704214) when young, tannish brown (#D2B48C) to light brown (#AC8260) at maturity, paler towards margin with age; context solid when young, spongy with age, white, without discoloration when bruised. Hymenophore poroid, adnate when young, slightly depressed around the apex of stipe with age; surface yellowish brown (#AD8F77) to pinkish brown (#E3B7AC), staining nude (#BF7258) when bruised; pores angular, 0.6–1.1 mm wide; tubes 6–14 mm long, concolorous with hymenophoral surface, slowly staining deep brown (#8E5627) when bruised or aging. Stipe 4–6.8 × 2.1–2.3 cm, clavate to subcylindrical, moderately enlarged downwards when young, attenuate at middle at maturity; surface predominantly concolorous with pileus, distinctly reticulate over upper portion, slowly staining brownish red when touched; context solid, pallid, slowly staining brown (#8C794E, #5A3E29) when bruised; basal mycelium pallid to yellowish. Spore print not obtained. Taste not obtained. Odor mild.

Basidiospores [200/3/3] (8.8) 10.1–12.0 (15.0) × (3.1–)3.5–4.1 (4.8) μm, Q = (2.39–)2.66–3.23 (–3.51), Q m = 2.94 ± 0.22, ellipsoid to cylindrical, smooth under light microscope and SEM, hyaline to yellowish brown in KOH, yellowish brown in Melzer’s reagent. Basidia 22–29 × 7–10 μm, clavate, 4-sterigmata, hyaline in KOH. Hymenophoral trama boletoid, composed of 8–11 μm wide cylindrical hyphae, thin-walled, hyaline in KOH. Cheilocystidia moderate, scattered, 43–56 × 8–13 μm, ventricose to ventricose-mucronate, thin-walled, yellowish brown in KOH. Pleurocystidia rare, scattered, 64–78 × 7–14 μm, fusiform to subfusiform, thin-walled, yellowish brown in KOH. Pileipellis a palisadoderm, composed of 6–10 wide μm hyphae, thin-walled, hyaline or yellowish in KOH, yellowish brown in Melzer’s reagent; terminal cells 24–54 (64) × 9.0–16.1 (20) μm, fusiform to subfusiform or ventricose, thin-walled, hyaline or yellowish to brownish in KOH. Pileal trama composed of 5–12 μm wide interwoven cylindrical hyphae, thin-walled, hyaline in KOH. Stipitipellis a cutis about 236 μm thick, composed of 3–7 μm wide cylindrical hyphae, thin walled, yellowish to brownish in KOH. Stipe trama composed of cylindrical hyphae 5–8 μm wide, thin-walled, hyaline in KOH. Clamp connections absent in all tissues.

Habitat:—Scattered to solitary on soil in subtropical forests dominated by Fabaceae and Fagaceae .

Distribution:—Currently known from southern China.

Additional specimens examined:— CHINA, GUANGDONG PROVINCE: Guangzhou City, Baiyun Mountain : 23°10′7″ N 113°17′31″ E, elev. 90 m, 5 June 2021, GDGM91091 , L.H. Qiu ; 23°10′7″ N 113°17′31″ E, elev. 119 m, 12 June 2021, B21061201 , X.X. Huang ; 23°10′3″ N 113°17′32″ E, elev. 88 m, 16 August 2021, GDGM91092 , L.H. Qiu ; 23°10′3″ N 113°17′29″ E, elev. 88 m, 4 September 2021, GDGM91093 , Z.J. Lin & Y.S. Liang .

Figures

FIGURE 3. Tylopilus pallidocervicolor: a–f. Basidiomata (a–c: GDGM91091; d–g: GDGM91094, holotype); h. Scanning electron micrograph of basidiospores (GDGM91094, holotype). Photos: a–b, d–g: L.H. Qiu; c: X.X. Huang; h: Z.J. Lin. Scale bars: b–c, e–g = 1 cm; h = 1 μm.

FIGURE 4. Microscopic features of Tylopilus pallidocervicolor (GDGM91094, holotype): a. Basidiospores; b. Cheilocystidia; c. Pleurocystidia; d. Basidia; e. Terminal cells of pileipellis. Scale bars: a–e = 10 μm. Drawings by Z.J. Lin.