Wagneromyia Vieira, Cordeiro, Bravo & Araújo, 2022

Vieira, Gabriel Santos, Cordeiro, Danilo Pacheco, Bravo, Freddy & Araújo, Maíra Xavier, 2022, A new genus of Psychodini (Diptera: Psychodidae) from the Neotropical region, Zootaxa 5188 (4), pp. 330-346 : 331-344

Wagneromyia Vieira, Cordeiro, Bravo & Araújo , gen. nov.

Type species: Wagneromyia aquiri Vieira, Cordeiro, Bravo & Araújo , sp. nov., by present designation.

Etymology. The genus name is in honor to Dr. Rüdiger Wagner, dipterologist and specialist in Psychodidae , with the Greek suffix myia meaning “fly”. The name is feminine.

Diagnosis. Eyes contiguous; antenna with 14 flagellomeres, nodiform (first to 11 th flagellomere), 12 th, 13 th, and 14 th flagellomeres reduced without internodes; setae alveoli of flagellomeres arranged in longitudinal rows; 14th flagellomere with one to three apical spiniform setula; palpus with four segments, fourth segment equal in length or lesser than the first; second segment of the palpus longer than the other segments; first flagellomere with a dorsal patch of sensilla; wing membrane pilose, with pilosity arranged parallel to the veins; gonocoxites contiguous at base; single aedeagus with lateral folds; epandrial appendage with three apical tenacula.

Description. Male. Head semicircular, eye bridge with four rows of facets; eyes contiguous; group of setae on the frons not reaching the lower limit of the eye bridge; interocular suture present or absent, when present small and U-inverted shape. antenna with cylindrical scape slightly longer than subspherical pedicel; 14 nodiform flagellomeres; pair of ascoids, Y-shaped, in each flagellomere; small additional sensilla near the ascoid insertion ( Fig. 5 ), on flagellomeres I-X; setae alveoli of flagellomeres arranged in longitudinal rows; flagellomeres 12 th, 13 th and 14 th reduced, separated from each other, without internodes; 11th, 13th with stout and spiniform setulae and 14th flagellomere with one to three apical spiniform setula; palpus with four segments, first of one with a group of dorsal sensilla near the base ( Fig. 3 ); length of fourth segment equal to or lesser than the first segment; second segment longer than the other segments. Labrum with convex dorsal margin, slightly expanded ( Fig. 3 ); bulbous labella with robust and spiniform setulae on the inner margin and setae on the lateral margin ( Fig. 4 ). Wing membrane pilose in lines parallel to the veins; C vein with two breaks at the base; Sc slightly curved at the apex, ending near the base of R 1; costal cell slightly infuscated; medial fork usually incomplete, the base of M 2 close to M 1 but always fading before joining M 1; sudden angulation at the end of R 2. Male terminalia with a narrow hypandrium, like a transversal band; gonocoxites usually dorsally contiguous; gonostylus conical, curved, with sparse setae and with an accessory long subapical seta; single aedeagus, narrow plate-like, with apex sometimes bifurcated and lateral folds; parameres of varied shapes, arising from the gonocoxal apodemes and articulated ventrally to the aedeagus at base; external lateral margin of the parameres generally projecting dorsally to the aedeagus forming a parameral sheath; parameral sheath of varied shape, connected to the gonocoxites, never exceeding the apex of the aedeagus; epandrium wider than long, with concave base and a large pseudospiracular opening; club-shaped epandrial appendages, with a group of setulae at the base, a group of subapical papilla on the dorsal surface and bearing three apical tenacula; hypoproct slightly projected and rounded, with pilose margin; lateral region of the hypoproct sclerotized and densely pilose, with setae a little longer than in the medial region; epiproct pilose and reduced.

Female. Unknown.

Distribution. South and Central America ( Costa Rica and Acre, Brazil).The current distribution of Wagneromyia is restricted to the Neotropical region.

Included species: Wagneromyia aquiri Vieira, Cordeiro, Bravo & Araújo , sp. nov., Wagneromyia bicalcarata ( Quate, 1996) comb. nov., Wagneromyia deceptrix ( Quate, 1996) comb. nov., Wagneromyia dimorpha ( Quate, 1996) comb. nov., Wagneromyia majorina ( Quate, 1996) comb. nov., Wagneromyia tripetala ( Quate, 1996) comb. nov., and Wagneromyia tritenacula ( Quate, 1996) comb. nov.

Remarks. Among the Psychodini , the bulbous labellum and symmetrical aedeagus, as found in Wagneromyia , are features shared with the genera Eurygarka , Feuerborniella , Mucomyia Kvifte & Curler, 2018 , Perithreticus Vaillant, 1973 , Quatiella Botosaneanu & Vaillant 1970 , Soeliella Kvifte, 2015 , Trichosepedon Krek, 1999 , and some species of Trichopsychoda Tonnoir, 1922 and Philosepedon . Thus, the species of Wagneromyia are easily differentiated from Psychodini genera that include species with laterally-compressed labella and/or an asymmetrical aedeagus, such as Psychoda , Epacretron Quate, 1965 , Neomaruina Vaillant, 1963 , Rhipidopsychoda Vaillant, 1991 , Nielseniella Vaillant, 1972 , Threticus Eaton, 1904 , and Cookiellocapsa Ježek & Le Pont, 2016 .

Wagneromyia and Eurygarka share the presence of contiguous eyes but Eurygarka has ascoids with leaf-shaped anterior branches, fourth segment of the palpus greater in length than the other segments, male terminalia with sclerotized and pilose ventral epandrial plate and two tenacula on the epandrial appendages. On the other hand, Wagneromyia species have ascoids with simple posterior branches, fourth segment of the palpus smaller than or equal to the first segment, weakly sclerotized and bare ventral epandrial plate, and three tenacula on the epandrial appendages.

Epandrial appendages with three tenacula, as in Wagneromyia can be found in Soeliella and some species transferred to Feuerborniella by Cordeiro et al. (2015), but the new genus can be recognized by the contiguous eye bridge, contrasting with the separate eyes in Feuerborniella and Soeliella , and by the length of the fourth segment of the palpus, smaller or equal to the first segment in the new genus and greater in length than the other segments in the other two genera. Furthermore, in Feuerborniella the terminal flagellomeres can be fused, while in Wagneromyia species they are separated, and in Soeliella , the aedeagus is wide and plate-shaped, being narrow and sometimes apically bifurcated in species of Wagneromyia .

The species of the genus Mucomyia , Perithreticus , Philosepedon , Quatiella , Trichopsychoda and Trichosepedon differ from Wagneromyia by the separate eyes, at least in the lower facet rows, and the length of the first segment of the palpus, which is smaller than the others.Also, in Mucomyia the male terminalia has gonostylus without subapical seta, Perithreticus has more than five tenacula, Philosepedon has two tenacula, Quatiella has only one tenaculum in the epandrial appendage, in Trichopsychoda the parameres are absent and in Trichosepedon the gonostylus has a longitudinal groove carrying a row of stout setae and the epandrial appendages have two tenacula. In contrast, in males of Wagneromyia the gonostylus has a subapical seta and no distinct longitudinal groove, the epandrial appendage has three tenacula, and parameres are present.

Omelková & Ježek (2012) proposed a monospecific subgenus Philothreticus Krek, 1999 for Philosepedon , with a contiguous eye bridge, but with the lower facet rows separated, ascoids with only two branches and asymmetric aedeagal complex, different from the new genus with all the rows of facets touching in the midline, ascoids with three branches, and the aedeagal complex symmetrical.

A feature observed for most species of Wagneromyia is the labrum with a convex and slightly expanded dorsal margin. This characteristic has been only observed in this genus until now, but a review of this structure is still needed regarding the other genera of the tribe, as this structure was rarely described and illustrated in previous works. Furthermore, as described for Quatiella truncata Chagas & Cordeiro, 2015 and species of Feuerborniella auctt ( Cordeiro et al. 2015; Pereira et al. 2015), the species of the new genus present an extension of the gonocoxal apodemes (= fused parameres), which surround the aedeagus to form a dorsal sheath, but which do not completely cover the aedeagus as in Brachycera (see Cumming & Wood 2017 for comments). Quate (1996) cited this structure, referring to it as projections of the anterior gonocoxal apodeme, Ibáñez-Bernal (2004) described it as the aedeagal sheath and Kvifte (2019) named these structures as parameral dorsal bridge. Although still not properly named, this is observed for other genera in illustrations (e.g. Kvifte et al. 2016, figs. 4, 8), however, it has gone unnoticed in works and therefore it is necessary to be revised in Psychodini species to understand how diagnostic it can be for the tribe’s genera.

Wagneromyia aquiri Vieira, Cordeiro, Bravo & Araújo , sp. nov.

( Figs 1–9 )

Type material: holotype ♂. BR [ Brazil], Acre, 15 Km a SE do Rio Branco, Embrapa , 170m [altitude], 10º01’S 67º41’N, 7–16.VII.2008, Malaise [trap] G. Melo [leg.] ( MZFS) ; paratypes 9 ♂, same date and locality as holotype ( MZFS) .

Etymology. The specific name, aquiri , refers to “a’quiri” or “a’kiru”, from the word uwákürü, from the dialect of the Ipurinã tribe, the original inhabitants of the region, from which the toponym Acre was derived. Acre is the name of the main river of the region and also the name of the Brazilian state where the species was collected. The name should be treated as a noun in apposition.

Diagnosis. Medial fork incomplete; R 2+3 1.2x length of R 2; apex of the gonostylus bent, with a small apical accessory seta and a long subapical seta on the ventral side; aedeagus straight, bending ventrolaterally to form a tubular-like structure that narrows to a small, bifurcated apex; parameres well sclerotized, broad at the base and tapering to a hook-shaped apex, curved outwards; parameral sheath subrectangular, with concave posterior margin; epandrial appendages with a group of five subapical papillae on the dorsal surface; hypoproct pilose with straight posterior margin and rounded vertices.

Description. Male. Head ( Fig. 1 ) as in genus description; eye bridge with a small medial spur on the dorsal margin and five widely spaced supraocular setae; group of setae on frons does not extend to the lower limit of the eye bridge; frontoclypeal suture present, as a sclerotized area; antenna incomplete in all specimens, flagellomeres beyond 10th lost in holotype; scape, pedicel, flagellomeres, and ascoids as in the genus description; palpus formula 1.0:1.6:1.2:1.0 first segment with a dorsolateral group of sensilla in the middle ( Fig. 3 ); clypeus with four to five prominent lateral alveoli and slightly concave apical margin; labella with four robust spiniform setulae on the inner margin, and three setae on the lateral margin. Wing ( Fig. 2 ) as in genus description; medial fork incomplete; R 2+3 1.2x the length of R 2. Male terminalia ( Figs. 6–9 ) with a narrow band-shaped hypandrium, wider medially; gonocoxites as in genus; gonostylus, with a sudden angulation near the apex, bearing a small apical accessory seta and a long subapical seta on the ventral surface ( Figs. 6, 7 ); aedeagus bending ventrally on the sides forming a tubular-like structure with a tapered and bifurcated apex on the ventral view ( Figs. 6, 7 ); parameres well sclerotized, broad at the base and tapering to a hook-shaped apex, curved outwards; parameres meet each other dorsally to the aedeagus forming a band-shaped parameral sheath with a small concavity at the midline ( Fig. 6 ); epandrium as in genus; epandrial appendages as in genus, with a group of five subapical papillae on the dorsal surface and three apical tenacula with half the length of the appendages ( Fig. 8 ); hypoproct pilose with straight posterior margin and rounded vertices ( Fig. 8 ); epiproct as in genus ( Fig. 9 ); subepandrial sclerite membranous.

Female. Unknown.

Wagneromyia bicalcarata ( Quate, 1996) comb. nov.

( Figs 10–16 )

Philosepedon bicalcaratus Quate, 1996: 58 .

Material examined: holotype ♂ ( Fig. 16 ). COSTA RICA, Heredia, Pr. Puerto Viejo de Sarapaqui , Est. Biol. LaSelva, Lt. Trp 22-25/VII/93, L. W. Quate. ( INBIO CRI001468679 ) .

Diagnosis. Medial fork incomplete, M 2 not reaching M 1; gonostylus apically bifurcated, like a chela; aedeagus with slightly enlarged bifid apex; subrectangular parameres apically bifurcated with an external arm like a hook and internal arm rounded; parameral sheath absent; hypoproct with medial lobe subtriangular in dorsal view.

Redescription. Male (holotype). Head ( Fig. 10 ) as in genus description; eye bridge with small medial dorsal spur and six supraocular setae, the innermost three closest; group of setae on frons extending medially close to the limit of the inferior margin of the eye bridge; frontoclypeal suture as a slightly sclerotized area, incomplete laterally; clypeus with three prominent lateral alveoli and slightly concave apical margin; labella with four robust spiniform setulae on the inner margin, and one seta on the lateral margin; antenna ( Fig. 11 ) as in genus; palpus formula 1.0:1.5:1.3:0.8, first segment of palpus with a group of sensilla at the slightly inflated base. Wing ( Fig. 12 ) as in genus; radial fork distal to medial fork; R 2+3 1.5x the length of R 2; M 2 not reaching M 1 (medial fork incomplete). Male terminalia ( Figs. 13–15 ) with hypandrium narrow, like a strip; gonocoxites as in genus; gonostylus apically bifurcated ( Fig. 13 ), with two short and strong projections like a chela, and with a long subapical seta on the ventral margin; aedeagus with slightly enlarged bifid apex; parameres subrectangular ( Figs. 13, 14 ), with the apical portion bifurcated into external arm hook-like, positioned dorsally to the aedeagus ( Fig. 13 ), and an internal arm rounded, lateral to the aedeagus ( Fig. 14 ); parameres not developed into a parameral sheath; epandrium ( Fig. 15 ) damaged and in lateral view in the holotype; epandrial appendages ( Fig. 15 ) curved in lateral view, base wider than the apex, with a group of three subapical papillae on the dorsal margin and tree apical tenacula with half the length of the appendices; epiproct short; hypoproct with medial lobe ( Fig. 15 ).

Female. Unknown.

Remarks. Although Quate (1996) described W. bicalcarata with separated gonocoxites, the examination of the holotype shows that they are contiguous as in the other species of the genus.

Wagneromyia deceptrix ( Quate, 1996) comb. nov.

( Figs 17–24 )

Philosepedon deceptrix Quate, 1996: 58 .

Material examined: holotype ♂ ( Fig. 24 ). COSTA RICA, Heredia Vara Blanca , 1800m, 26/VII/ 93 L. W. Quate, 1800m, Lt Trp, Euparal, LN —239.500-519.000 ( INBIO CRI001469462 ) .

Diagnosis. 14th flagellomere with two apical spiniform setulae; medial fork complete; subtriangular parameral sheath with a rounded apex; aedeagus straight, with bifid apex, like two ventral lobes; parameral sheath subtriangular with a rounded apex; anterior gonocoxal apodemes with setae on the posteromedial margin, continuous with the ventral setae of the gonocoxite; hypoproct pilose and subovalate.

Redescription. Male (holotype). Head ( Fig. 17 ) as in genus description; eye bridge with a very short dorsal medial spur and five supraocular setae; group of setae on frons not extending medially; frontoclypeal suture only as a slightly less sclerotized area; clypeus with three prominent lateral alveoli and slightly concave apical margin; labella with four robust spiniform setulae on the inner margin, and four setae on the external lateral margin, the most apical one shorter than the others; antenna ( Fig. 19 ) as in genus; 14 th flagellomere with two apical stout and spiniform setulae; palpus formula: 1.0:1.6:1.2:0.8, first segment of palpus with a group of sensilla near the base. Wing ( Fig. 18 ) as in genus description; radial fork distal to medial fork, both complete; R 2+3 1.3x the length of R 2. Male terminalia ( Figs. 20–23 ) with hypandrium as a narrow band, little sclerotized; gonocoxite with setae on the posteromedial dorsal margin lined up near the base; gonostylus as in genus description; aedeagus ( Figs. 20, 21 ) straight, with bilobed apex; parameres ( Fig. 21 ) with a membranous (non-sclerotized) region between them and the gonocoxal apodemes; parameres with a broad base from where it projects dorsally to the aedeagus forming a subtriangular parameral sheath with a rounded apex ( Fig. 20 ); anterior gonocoxal apodemes with setae on the posteromedial margin, continuous with the ventral group of setae of the gonocoxite ( Fig. 21 ); epandrium ( Fig. 22 ) typical for genus; epandrial appendages ( Fig. 23 ) as in genus description, with a group of three subapical papillae on the dorsal margin and three apical tenacula half the length of the appendices; epiproct ( Fig. 22 ) small and rounded; hypoproct ( Fig. 23 ) pilose and subovalate.

Female. Unknown.

Wagneromyia dimorpha ( Quate, 1996) comb. nov.

( Figs 25–30 )

Philosepedon dimorphus Quate, 1996: 58 .

Material examined: holotype ♂ ( Fig. 30 ). COSTA RICA, Heredia, Puerto Viejo de Sarapaqui , Est. Biol. LaSelva, Lt. Trp 23/VII/93, L. W. Quate, 50- 100m . ( INBIO CRI001468641 )

Diagnosis. Medial fork incomplete; gonostylus with small subapical spines lined on the ventrolateral margin and a long subapical seta; aedeagus with a straight and tapered bifid apex; parameres long, robust, losing sclerotinization as they approach an outward hook-like apex; parameral sheath poorly sclerotized, as a narrow and membranous stripe.

Redescription. Male. Head ( Fig. 25 ) as in genus description; eye bridge with a small medial spur and five supraocular setae, the two or three innermost closest; group of setae on frons extending medially; frontoclypeal suture only as a slightly less sclerotized area; antenna incomplete in holotype, flagellomeres beyond 7th lost; ascoids lost in holotype; clypeus with three prominent lateral alveoli and slightly concave apical margin; labella with four robust spiniform setulae on the inner margin and one seta on the external lateral margin; palpus formula 1.0:1.4:1.3:0.9, first segment of palpus as in genus description. Wing ( Fig. 26 ) as in genus description; complete radial fork distal to an incomplete medial fork; R 2+3 1.3x the length of R Male terminalia ( Figs. 27–29 ) with hypandrium as a narrow band; gonocoxites as in genus description; gonostylus with small subapical spines lined on the ventrolateral margin and a long subapical seta; aedeagus straight and bifid on distal half; parameres ( Figs. 27, 28 ) reaching the apex of aedeagus, robust and wide at the base, losing sclerotinization as they approach an outward hook-like apex; parameral sheath ( Fig. 27 ) poorly sclerotized, like a narrow and membranous stripe; epandrium ( Fig. 29 ) as in genus description, but damaged; epandrial appendages with a group of short setulae on the base, a group of three subapical papillae on the dorsal margin and three apical tenacula half the length of the appendices; epiproct ( Fig. 29 ) short; hypoproct not very pronounced.

2.

Female. Unknown.

Wagneromyia majorina ( Quate, 1996) comb. nov.

( Figs 31–37 )

Philosepedon majorinus Quate, 1996: 60 .

Material examined: holotype ♂ ( Fig. 37 ). COSTA RICA, Heredia, S Rafael de Vara Blanca, Rio Santo Dominigo , 18-26/VII/ 93 L. W. Quate, 1700m, Lt Trp, Euparal. ( INBIO CRI001468528 ) .

Diagnosis. Frontoclypeal suture absent; medial fork complete; aedeagus with bifid and straight apex; parameres broad at the base, not extending posteriorly; parameral sheath bilobed.

Redescription. Male. Head ( Fig. 31 ) as in genus description; eye bridge with dorsal medial spur and five to six supraocular setae; group of setae on frons not extending medially to the base of eye bridge; frontoclipeal suture absent; antenna ( Fig. 32 ) as in genus description; clypeus with four prominent lateral alveoli and slightly concave apical margin; labella with robust spiniform setulae on the inner margin; palpus formula 1.0:1.4:1.2:0.9. Wing ( Fig. 33 ) as in genus description; radial fork complete, distal to complete medial fork; R 2+3 1.5x the length of R 2. Male terminalia ( Figs. 34–36 ) with hypandrium reduced to a narrow band wider medially, poorly sclerotized; gonocoxite with setae on the posteromedial dorsal margin, lined up near the base; gonostylus as in genus description; aedeagus ( Fig. 34 ) with bifid and straight apex; parameres ( Fig. 35 ) united to the gonocoxal apodemes by a membranous area; parameres broad at the base, not extending posteriorly, projecting dorsally to the aedeagus to form a bilobed parameral sheath ( Fig. 34 ); anterior gonocoxal apodemes without setae; epandrium and appendages ( Fig. 36 ) as in genus description; epandrial appendages with a group of three subapical papillae on the dorsal margin and with three apical tenacula longer than half the length of the appendices; epiproct inconspicuous and hypoproct slightly pronounced, wider than long.

Female. Unknown.

Wagneromyia tripetala ( Quate, 1996) comb. nov.

( Figs 38–44 )

Philosepedon tripetalis Quate, 1996: 60 .

Material examined: holotype ♂ ( Fig. 44 ). COSTA RICA, Heredia Vara Blanca , 1800m, 26/VII/ 93 L. W. Quate, 1800m, Lt Trp, Euparal, LN —239.500-519.000 ( INBIO CRI001469454 ) .

Diagnosis. Medial fork incomplete; gonocoxites fused at the base; posterior gonocoxal apodemes forming a highly sclerotized ring, dorsal to the aedeagus and the parameral sheath; parameral sheath membranous and rounded; parameres well sclerotized, broad at based tapering to a hook-shaped apex; hypoproct little pronounced, ellipsoid, wider than long.

Redescription. Male. Head ( Fig. 38 ) as in genus description; eye bridge without medial dorsal spur and with seven supraocular setae; fourth row of facet nearly separated; group of setae on frons not extending medially toward base of eye bridge; frontoclipeal suture only as a slightly less sclerotized area; clypeus with nine prominent lateral alveoli and slightly concave apical margin; palpus formula 1.0:1.5:1.4:0.9, first segment of palpus with a group of sensilla in the medial region; labella with four robust spiniform setulae on the inner margin, and six setae on the external lateral margin; antenna ( Fig. 39 ) as in genus description. Wing ( Fig. 40 ) as in genus description; radial fork complete, distal to incomplete medial fork; R 2+3 1.4x the length of R 2. Male terminalia ( Figs. 41–43 ) with hypandrium as a narrow band, a little wider medially; gonocoxites ( Fig. 41 ) fused at the base, with setae on the posteromedial dorsal margin lined up near the base; gonostylus as in genus description; aedeagus narrow, tapering to a single apex; posterior gonocoxal apodeme fused medially, forming a highly sclerotized ring—parameral sclerite, dorsal to the aedeagus and the parameral sheath ( Fig. 41 ); parameres continuous with the posterior gonocoxal apodemes; parameres ( Figs. 41, 42 ) well sclerotized, broad at based tapering to a hook-shaped apex; membranous parameral sheath with a rounded apex ( Fig. 41 ); anterior gonocoxal apodemes ( Fig. 42 ) with setae on the posteromedial margin, continuous with the ventral setae of the gonocoxite; epandrium ( Fig. 43 ) as in genus description; epandrial appendages as in genus description with a group of three subapical papillae on the dorsal margin and three apical tenacula half the length of the appendices; epiproct inconspicuous and hypoproct ( Fig. 43 ) not very pronounced, ellipsoid, wider than long, both pilose.

Female. Unknown.

Wagneromyia tritenacula ( Quate, 1996) comb. nov.

( Figs 45–51 )

Philosepedon tritenaculus Quate, 1996: 58 .

Material examined: holotype ♂ ( Fig. 51 ). COSTA RICA, Guanac. Estacion Pitilla, 700m. 9 Km S Santa Cecilia, 10-14/VII/93, L. W. Quate, Light Trap Euparal. ( INBIO CRI001469215 ) .

Diagnosis. 14th flagellomere with three apical stout and spiniform setulae; medial fork complete; aedeagus with lateral folds subapically, forming an abrupt bottleneck anterior to a bifurcated apex; parameres broad at the base, not extending posteriorly; parameral sheath trapezoidal; gonocoxal apodemes with four setae posteriorly.

Redescription. Male. Head ( Fig. 45 ) as in genus description; eye bridge without medial dorsal spur and with five to six supraocular setae; group of setae on frons extending medially near the inferior limit of the eye bridge; frontoclipeal suture only as a slightly less sclerotized area; clypeus with three prominent lateral alveoli and slightly concave apical margin; labella with four robust spiniform setulae on the inner margin and three setae on the external lateral margin; antenna ( Fig. 46 ) as in genus description; palpus formula 1.0:1.5:1.2:0.8, first segment of palpus with a group of sensilla near the base. Wing ( Fig. 47 ) as in genus description; radial fork complete, distal to the complete medial fork; R 2+3 1.4x the length of R 2. Male terminalia ( Figs. 48–50 ) with hypandrium reduced to a narrow band; gonocoxite with setae on the posteromedial dorsal margin lined up near the base; gonostylus as in genus description; aedeagus ( Figs. 48, 49 ) with subapical lateral folds, forming a bottleneck anterior to the bifurcated apex; parameres united to the gonocoxal apodemes by a membranous area; parameres ( Fig. 49 ) broad at the base, not extending posteriorly, projecting dorsally to the aedeagus to form a trapezoidal parameral sheath ( Fig. 48 ); gonocoxal apodema with four setae posteriorly; epandrium ( Fig. 50 ) as in genus description; epandrial appendages with a group of short setulae on the base, a group of three subapical papillae on the dorsal margin and three apical tenacula of half the length of the appendices; epiproct inconspicuous and hypoproct ( Fig. 50 ) slightly pronounced, wider than long

Female. Unknown.

Identification key for males of Wagneromyia species

The species of the new genus are present in the key of Quate (1996) to the genus Philosepedon . A new key is provided below to identify the new described species and the species transferred to the new genus.

1 Gonostylus ending in a single and narrow apex; each paramere with a non-bifurcated apex........................... 2

- Gonostylus ending apically bifurcated, like a chela; each paramere apically bifurcated in one external arm hook-like, dorsal to the aedeagus, and an internal arm rounded...................................................... W. bicalcarata

2 Wing with medial fork incomplete; gonocoxites with a group of setae concentrated in the dorsal posteromedial region; parameres well developed, reaching the level of the aedeagus apex...................................................... 3

- Wing with medial fork complete; gonocoxites with a group of setae in a row from the apex to near the base; parameres not extending posteriorly ventral to the aedeagus............................................................... 5

3 Presence of medial dorsal spur on the eye bridge; fourth facet row completely contiguous; gonocoxites contiguous, posterior gonocoxal apodeme not extended medially; parameral sheath wider than long; aedeagus with bifurcated apex........... 4

- Medial dorsal spur on the eye bridge absent; fourth facet row nearly separated; gonocoxites continuous, with posterior gonocoxal apodemes extended and fused medially; parameral sheath as long and wide at the base; aedeagus with single apex................................................................................................... W. tripetala

4 Clypeus with five prominent lateral alveoli; gonostylus bent in the subapical region, bearing a small apical accessory seta and a long subapical seta on the ventral side; parameres greatly sclerotized............................ W. aquiri sp. nov.

- Clypeus with three prominent lateral alveoli; gonostylus slightly curved through its extension, with a row of small subapical spines on the ventrolateral margin and a long subapical seta, almost at the apex; parameres with reduced sclerotization while approaching the apex........................................................................ W. dimorpha

5 14th flagellomere with more than one apical spiniform setula; eye bridge without medial dorsal spur; parameral sheath apex with single lobe; anterior gonocoxal apodemes with setae on the posterior region, continuous with the ventrolateral setae of the gonocoxite; length of tenacula equal to half the length of the epandrial appendages................................. 6

- 14th flagellomere with one apical spiniform setula; eye bridge with medial dorsal spur; parameral sheath apex bilobed; gonocoxal apodemes without setae in the posterior region; tenacula longer than half the length of the epandrial appendages.......................................................................................... W. majorina

6 14th flagellomere with two apical spiniform setulae; group of setae on frons not extending medially; apex of parameral sheath with rounded lobe; aedeagus with margins slightly concave before apex............................... W. deceptrix

- 14th flagellomere with three apical setulae; group of setae on frons extending medially near the base of eye bridge; apex of parameral sheath truncated; aedeagus with sinuous margins like a bottleneck........................... W. tritenacula

Figures

FIGURES 1–5. Wagneromyia aquiri, sp.nov. 1. Head.2.Wing.3. Mouthparts.4. Labellum (ventral view).5. Third flagellomere. Abbreviations: asc—ascoid; plp 1 sen—first palpus sensilla; flgm sen—flagellomere sensilla; lbr—labrum.

FIGURES 6–9. Wagneromyia aquiri, sp. nov. 6. Gonopods and aedeagal complex (dorsal view). 7. Gonopods and aedeagal complex (ventral view). 8. Epandrium (dorsal view). 9. Epandrium (ventral view). Abbreviations: aed—aedeagus; epand app— epandrial appendages; epiprct—epiproct; goncx apod—gonocoxal apodemes; hypd—hypandrium; hyprct—hypoproct; pm— paramere; pm sh—parameral sheath; sbepand scl—subepandrial sclerite.

FIGURES 10–16. Wagneromyia bicalcarata. 10. Head. 11.Terminal flagellomeres.12.Wing.13. Male terminalia (dorsal view). 14. Male terminalia (ventral view). 15. Epandrium and epandrial appendages (lateral view). 16. Holotype labels. Abbreviations: aed—aedeagus; goncx apod—gonocoxal apodemes; hyprct—hypoproct; pm—paramere.

FIGURES 17–24. Wagneromyia deceptrix. 17. Head. 18. Wing. 19. Terminal flagellomeres. 20. Gonopods and aedeagal complex (dorsal view). 21. Gonopods and aedeagal complex (ventral view). 22. Epandrium (ventral view). 23. Epandrial appendages (lateral view). 24. Holotype labels. Abbreviations: aed—aedeagus; epiprct—epiproct; goncx apod—gonocoxal apodemes; hyprct—hypoproct; pm—paramere; pm sh—parameral sheath.

FIGURES 25–30. Wagneromyia dimorpha. 25. Head. 26. Wing. 27. Gonopods and aedeagal complex (dorsal view). 28. Gonopods and aedeagal complex (ventral view). 29. Epandrium and epandrial appendages. 30. Holotype labels. Abbreviations: aed—aedeagus; epiprct—epiproct; goncx apod—gonocoxal apodemes; pm—paramere; pm sh—parameral sheath.

FIGURES 31–37. Wagneromyiamajorina. 31.Head.32.Terminal flagellomeres.33. Wing.34. Gonopods andaedeagal complex (dorsal view). 35. Gonopods and aedeagal complex (ventral view). 36. Epandrium and epandrial appendages. 37. Holotype labels. Abbreviations: aed—aedeagus; goncx apod—gonocoxal apodemes; pm—paramere; pm sh—parameral sheath.

FIGURES 38–44. Wagneromyia tripetala. 38.Head. 39.Terminal flagellomeres.40. Wing. 41. Gonopods and aedeagal complex (dorsal view). 42. Gonopods and aedeagal complex (ventral view). 43. Epandrium and epandrial appendages (ventral view). 44. Holotype labels. Abbreviations: aed—aedeagus; goncx apod—gonocoxal apodemes; hyprct—hypoproct; pm—paramere; pm scl—parameral sclerite; pm sh—parameral sheath.

FIGURES 45–51. Wagneromyia tritenacula. 45. Head. 46. Terminal flagellomeres. 47. Wing. 48. Gonopods and aedeagal complex (dorsal view). 49. Gonopods and aedeagal complex (ventral view). 50. Epandrium and epandrial appendages (dorsal view). 51. Holotype labels. Abbreviations: aed—aedeagus; goncx apod—gonocoxal apodemes; hyprct—hypoproct; pm— paramere; pm sh—parameral sheath.