Zygophiala montenegroensis G.Y. Sun, Mian Zhang, 2015

Zhang, Mian, Gao, Liu, Shang, Shengping, Han, Xiaolu, Zhang, Rong, Latinović, Jelena, Latinović, Nedeljko, Batzer, Jean C., Gleason, Mark L. & Sun, Guangyu, 2015, New species and record of Zygophiala (Capnodiales, Mycosphaerellaceae) on apple from Montenegro, Phytotaxa 195 (3), pp. 227-235 : 231

Zygophiala montenegroensis G.Y. Sun, Mian Zhang , sp. nov. ( Figs. 3 , 4 ) MycoBank MB 810371

Holotype:— MONTENEGRO. Berane , from apple fruit ( Malus ×domestica ), Sep. 2013, G. Y. Sun & J. Latinović, dried culture HMAS (245580); living cultures from holotype — CGMCC 3.17372 (= culture ZMHS29 ).

Other specimens examined: — MONTENEGRO. Berane, from apple fruit ( Malus ×domestica ), Dec. 2013, G. Y. Sun & J. Latinović, dried culture ZMHS 35.

Colony of isolate ZMHS 29 on the cuticle of apples consisted of shiny, black, round or ovoid, sclerotium-like bodies without visible mycelial mat; hyphae hyaline, reaching 1.2–2.5 μm wide on PDA; colony slow growing, reaching 6–11 mm diameter after 1 month on PDA, granular, overall uplift, mountainous, margins irregular with many small yellow or dark droplets in the center; white, grey and brown throughout; basal cell long and thin, upright, light colour; Conidiophores arising from superficial hyphae, erect, scattered, subcylindrical, flexuous, (19–)24–31(–45) × (3–)5– 7(–9) μm (from basal septum to below phialide), consisting of a hyaline to subhyaline supporting cell that gives rise to a smooth, dark brown cell, and a finely verruculose, medium brown, apical cell (3–)5–7.5(–10) × (2–)4–7(–9) μm from which arise 2 conidiogenous cells. Conidiogenous cells long and thin, (7–)9–13(–18) × (2–)5–6(–8) μm, light brown, doliiform to elipsoidal, smooth, growing from the apical cell; scars prominent, apical, darkened, thickened, to some degree refractive, 2–3 μm wide. Conidia solitary, subcylindrical, hyaline, smooth, thick-walled, (0–)1-septate; 0-septate, (5–)9–15(–17) × (5–)6–9(–11) μm, 1-septate, (10–)13–25(–34) × (3–)5–6(–8) μm, constricted at septum.

Notes: — Z. montenegroensis is distinguished from other species, except Z. cylindrical , by its subcylindrical (0–)1-septate conidia and large conidiogenous cells ( Table 2). The conidia of Z. montenegroensis are smaller than those of Z. cylindrica and are (0–)1-septate whereas those of Z. cylindrica are (0–)1(–2)-septate. The conidiogenous cells of Z. montenegroensis are larger than those of Z. cylindrica ( Table 2). Furthermore, Z. montenegroensis and the other species formed distinct clades both in the ITS tree and the ITS-ACT-TEF matrix tree, with strong bootstrap support in both trees. As a result, Z. montenegroensis is considered to be a new species.

Figures

FIGURE 3. Zygophiala montenegroensis ZMHS29. a. 1-month-old colony on PDA.b.Flyspeck signs on apple peel.c, e, g. Conidiophores, conidiogenous cells and conidia. f. Conidiophore. d, h–k. Conidia. Scale bars = 5 μm.

FIGURE 4. Conidiophores, conidiogenous cells and conidia of Zygophiala montenegroensis ZMHS29. Scale bar = 10 μm.