Ceratocystiopsis synnemata B. Strzalka , R. Jankowiak & G. Hausner, sp. nov. MycoBank No: 835151

Strzalka, Beata, Jankowiak, Robert, Bilanski, Piotr, Patel, Nikita, Hausner, Georg, Linnakoski, Riikka & Solheim, Halvor, 2020, Two new species of Ophiostomatales (Sordariomycetes) associated with the bark beetle Dryocoetes alni from Poland, MycoKeys 68, pp. 23-48 : 23

Ceratocystiopsis synnemata B. Strzalka, R. Jankowiak & G. Hausner, sp. nov. MycoBank No: 835151 Fig. 5

Etymology.

The epithet ( synnemata ) refers to the synnematous conidiomata formed by this fungus.

Type.

Poland, Paprocice, from Dryocoetes alni beetle infesting Populus tremula , 5 Oct 2018, K. Miśkiewicz (TUR 207995 http://mus.utu.fi/TFU.207995 holotype, ex-holotype cultures: NRIF 16918DA = KFL 16918DA).

Description.

Sexual morph: not observed. Asexual morph: hyalorhinocladiella-like. Conidiophores micronematous or macronematous. The micronematous conidiophores, hyaline, consist of conidiogenous cells arising singly from the vegetative hyphae (6-)8.6-16.4(-23.2) × (0.6-)0.9-1.3(-1.6) µm. The macronematous conidiophores are much larger, (14.5-)17.3-39.8(-76.9) µm long than the preceding forms and from a basal cell, (3.1-)5.3-11.2(-17) × (0.9-)1.1-1.9(-2.6) µm. The basal cells branch lateral or penicillate and form 1-5 branches (mostly 1-2) producing conidiogenous cells at their apices. Conidiophores often aggregate in loosely synnemata , (43.2-)52.3-86.4(-114.7) µm long, (2.4-)3.6-8.2(-12.9) µm wide at the tip. Conidia hyaline, smooth, unicellular, oblong-elliptical, (2.4-)2.8-3.5(-4) × (1-)1.1-1.3(-1.4) µm. Cultural characteristics: Colonies with optimal growth at 25 °C on 2% MEA with radial growth rate 1.4 ( ± 0.1) mm/d, growth very well at 30 °C (1.3 mm/d) and 35 °C (1.0 mm/d). Colonies yellowish gray, margin smooth. Hyphae pale gray in color, smooth, submerged in the medium and aerial mycelium rare, not constricted at the septa, 0.4-2.6 (mean 1.1 ± 0.6) µm diam., asexual morph moderately abundant, very abundant after adding twigs.

Host trees.

Alnus incana , Populus tremula

Insect vector.

Dryocoetes alni

Distribution.

Poland

Note.

Ceratocystiopsis synnemata can be distinguished from C. pallidobrunnea by the shape and size of the conidia. Ceratocystiopsis synnemata has shorter and oblong-elliptical conidia in contrast to the allantoid conidia of C. pallidobrunnea ( C. synnemata : 2.4-4 × 1-1.4 µm; C. pallidobrunnea : 2.5-5 × 0.7-1.2 µm ( Olchowecki and Reid 1974), 2-7 × 0.7-2.5 µm ( Upadhyay 1981) (Table 3 ). In addition, C. synnemata produces conidiophores that aggregate into loosely arranged synnemata .

Figures

Figure 5. Ceratocystiopsis synnemata sp. nov. (NRIF 16918 DA = KFL 16918 DA) a, b micronematous conidiophores c-e macronematous conidiophores f, g conidiophores aggregate in synnemata h conidia i fourteen-day-old culture on MEA. Scale bars: 25 μm (a), 10 μm (b), 25 μm (c-g), 10 μm (h).