New records of Tardigrada from Bulgaria with the description of Macrobiotus binieki sp. nov. (Eutardigrada: Macrobiotidae) and a key to the species of the harmsworthi group Author Kaczmarek, Łukasz Author Gołdyn, Bartłomiej Author Prokop, Zofia M. Author Michalczyk, Łukasz text Zootaxa 2011 2781 29 39 journal article 47870 10.5281/zenodo.206999 f488c32f-c9a5-4c9c-9326-75a0ec9e287b 1175-5326 206999 Key to the species of the harmsworthi group 1. Cuticle either with pores, or granulation, or stripes of pigmentation............................................. 2 -. Cuticle smooth....................................................................................... 9 2(1). Pores in cuticle present, granulation and stripes of pigmentation absent........................................... 3 -. Pores in cuticle absent, granulation or stripes of pigmentation present............................................ 4 3(2). Egg shell smooth, processes in the shape of low cones (mammillate-like) without finger-like structures at the process base, bubble-like structures at the top of processes present, 16–21 processes on the egg circumference........................ ....................................................................... M. krynauwi Dastych & Harris, 1995 -. Egg shell covered with dots, processes in the shape of high cones with finger-like structures at the process base, bubble-like structures at the top of processes absent, 6–8 processes on the egg circumference.............. M. liviae Ramazzotti, 1962 4(2). Nine bands of brown pigmentation on cuticle present*................................... M. baltatus McInnes, 1991 -. Cuticle without bands of pigmentation..................................................................... 5 5(4). Granulation on cuticle in the shape of large tubercles (1.5–2.0), present only on the caudal end of the body........................................................................................ M. arguei Pilato & Sperlinga, 1975 -. Granulation present on the entire dorsal side of the body....................................................... 6 6(5). Granulation on cuticle in the shape of small tubercles, eyes present.............................................. 7 -. Granulation on cuticle in the shape of fine dots; eyes absent................................................... 8 7(6). First band of teeth in the oral cavity armature and granulation on legs I–III absent, lunules IV smooth.............................................................................................. M. perfidus Pilato and Lisi, 2009 -. First band of teeth in the oral cavity armature and granulation on legs I–III present, lunules IV dentate......................................................................................... M. pseudocoronatus Pilato et al., 2006 8(6). Areolae around the bases of egg processes absent, egg processes smooth, in the shape of spines with a wide base and a sharp apex, processes smooth, 22–23 processes on the egg circumference....................... M. contii Pilato & Lisi, 2006 -. Areolae around the bases of egg processes present, egg processes in the shape of cones with a blunt apex, processes reticulated, 10–12 processes on the egg circumference.................................... M. pseudonuragicus Pilato et al., 2004 9(1). Spurs on claws present....................................................... M. armatus Pilato & Binda, 1996 -. Spurs on claws absent................................................................................. 10 10(9). Egg processes situated very close (in contact) one to another................. Macrobiotus neuquensis Rossi et al., 2009 -. Spaces between egg processes present.................................................................... 11 11(10). Areolation on eggs present............................................................................. 12 -. Areolation on eggs absent.............................................................................. 19 12(11). Each process inside a single areola...................................................... M. mauccii Pilato, 1974 -. A number of areolae present around each process........................................................... 13 13(12). Egg areolation poorly visible (areolae with thin walls), sometimes not fully developed, processes with a blunt apex...... 14 -. Egg areolation fully developed and well visible, processes with a sharp or indented apex............................ 15 14(13). Eyes present, bubble-like structures at the top of processes absent, egg processes in the shape of cones, egg shell covered by delicate stripes........................................................ M. harmsworthi obscurus Dastych, 1985 -. Eyes absent, bubble-like structures at the top of processes present, egg processes in the shape of a bells (domes), egg shell cov- ered by delicate dots.............................................................. M. peterseni Maucci, 1991 15(13). Body red, the oral cavity armature of the echinogenitus - type , lunules IV not thickened and without indentations, a crown of dots around the bases of processes present.................................... M. ovostriatus Pilato & Patanè, 1998 -. Body transparent, the oral cavity armature of the harmsworthi - type , lunules IV thickened or with indentations, a crown of dots around the bases of processes absent..................................................................... 16 16(15). Sixteen areolae around the base of each egg process............................. M. pseudoliviae Pilato & Binda, 1996 -. Six areolae around the base of each egg process........................................................... 17 17(16). Process top divided into a number of points, more than 13 processes on the egg circumference............................................................................................... M. nuragicus Pilato & Sperlinga, 1975 -. Process top with 1–2 points, less than 13 processes on the egg circumference..................................... 18 18(17). Eyes and granulation on legs I present, lunules IV thickened, pt of stylet supports less than 76% , egg processes without a flex- ible upper portion........................................................ M. hieronimi Pilato & Claxton, 1988 -. Eyes and granulation on legs I absent, lunules IV with indentations, pt of stylet supports more than 77% , egg processes with a flexible upper portion...................................................... M. barbarae Kaczmarek et al., 2007 19(11). Egg processes hemispherical............................................................................ 20 -. Egg processes conical................................................................................. 21 20(19). Egg shell with reticular sculpture.................................................... M. montanus Murray, 1910 -. Egg shell without reticular sculpture............................................... M. mottai Binda & Pilato, 1994 21(19). A well visible crown of swellings, dots or stripes around the bases of egg processes present......................... 22 -. A crown of swellings, dots or stripes around the bases of egg processes absent.................................... 28 22(21). Egg processes smooth....................................................... M. australis Pilato & D'Urso, 1976 -. Egg processes reticulated or with small round bubbles....................................................... 23 23(22). A crown around the bases of egg processes composed of rectangular swellings, processes in the shape of spines with a wide base, more than 25 processes on the egg circumference.......................................... M. binieki sp. nov. -. A crown around the bases of egg processes composed of dots or stripes, processes in the shape of low cones, less than 20 pro- cesses on the egg circumference........................................................................ 24 24(23). A crown around the bases of egg processes composed of radiate stripes forming a larger radiate crown, processes with a blunt, indented apex.................................................................. M. radiatus Pilato et al., 1991 -. A crown around the bases of egg processes composed of dots, processes with a sharp apex.......................... 25 25(24). Weakly visible granulation present only on legs IV, terminal portion of egg processes not flexible................................................................................................... M. rigidus Pilato & Lisi, 2006 -. Evident granulation present on legs I–IV, terminal portion of egg processes flexible................................ 26 26(25). Eyes absent................................................................... M. patiens Pilato et al., 2000