Diptacus rubuscolum Trinidad, Duarte & Navia (Eriophyoidea: Diptilomiopidae) on blackberry (Rubus ulmifolius Schott) in Mexico: occurrence, complementary description, and biology Author González-DomínguezK, Sandra G. Postgrado en Fitosanidad-Entomología y Acarología. Colegio de Postgraduados, km 36.5 Carretera Mexico-Texcoco, Montecillo, Texcoco, Edo. de Mexico, 56264, Mexico. Author Santillán-GaliciaK, Ma. Teresa Postgrado en Fitosanidad-Entomología y Acarología. Colegio de Postgraduados, km 36.5 Carretera Mexico-Texcoco, Montecillo, Texcoco, Edo. de Mexico, 56264, Mexico. Author Guzmán-FrancoK, Ariel W. Postgrado en Fitosanidad-Entomología y Acarología. Colegio de Postgraduados, km 36.5 Carretera Mexico-Texcoco, Montecillo, Texcoco, Edo. de Mexico, 56264, Mexico. Author García-AvilaK, Clemente de Jesús Centro Nacional de Referencia Fitosanitaria, km 37.5, Carretera Federal México-Pachuca, Tecámac, Edo. de México, C. P. 55740, Mexico. Author López-BuenfilK, José Abel Centro Nacional de Referencia Fitosanitaria, km 37.5, Carretera Federal México-Pachuca, Tecámac, Edo. de México, C. P. 55740, Mexico. Author Romero-Rosales, Felipe Postgrado en Fitosanidad-Entomología y Acarología. Colegio de Postgraduados, km 36.5 Carretera Mexico-Texcoco, Montecillo, Texcoco, Edo. de Mexico, 56264, Mexico. text Acarologia 2023 2023-06-27 63 3 793 806 http://dx.doi.org/10.24349/j7im-cara journal article 10.24349/j7im-cara 2107-7207 10786030 Biology of DIPtacus rubuscolum Diptacus rubuscolum has a simple life cycle with the same developmental stages typical of the Eriophyioidea superfamily: egg, larva, nymph and adult. They had two quiescent stages; the first was between the larval stage and the nymphal stage (nymphochrysalis), and the second was between the nymphal stage and the adult stage (imagochrysalis). The crystalline colour of the egg became yellow and translucent, and it took on a semi-spherical shape. Fertilized females oviposited on leaf surfaces, particularly along the secondary ribs. The mean duration of the egg stage was 56.5±2 h. Larvae were crystalline in colour and became translucent before the first quiescent stage; the mean duration of this stage was 56.5±2 h. The first quiescent stage, or nymphochrysalis, was white in colour with a mean duration of 21.8±1.5 h. There was only one nymphal stage, white in colour, which showed greater mobility and feeding activity than the larva; the mean duration of this stage was 41.3±1.9 h. The second quiescent stage, or imagochrysalis, was white in colour, and occurred just before the adult stage and had a mean duration of 19.7±1.3 h. The adult was white in colour and the most active stage with a mean duration of 114.5±2.7 h. Table 2 Percentage of divergence with standard error (SE) for D2 region of the 28S rDNA sequences pairs within and between Diptacus species and other members of Diptilomiopidae , Phytoptidae and Eriophyidae . Samples labelled as 1A and 1C D. are rubuscolum from Mexico. All other sequences were retrieved from GenBank and accession numbers are shown.