A new species of the genus Eutrombicula Ewing, 1938 (Trombidiformes: Trombiculidae) and new records for the species Eutrombicula batatas (Linnaeus, 1758) in Brazil Author Bassini-Silva, Ricardo Departamento de Medicina Veterinária Preventiva e Saúde Animal, FMVZ-USP, São Paulo, SP, Brazil. Author Jacinavicius, Fernado de Castro Departamento de Medicina Veterinária Preventiva e Saúde Animal, FMVZ-USP, São Paulo, SP, Brazil. & Laboratório Especial de Coleções Zoológicas, Instituto Butantan, São Paulo, SP; Brazil. Author Muñoz-Leal, Sebastián Departamento de Medicina Veterinária Preventiva e Saúde Animal, FMVZ-USP, São Paulo, SP, Brazil. Author Maturano, Ralph Departamento de Zoologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil. Author Welbourn, W. Calvin Florida State Collection of Arthropods, Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, FL, USA. Author Ochoa, Ronald Systematic Entomology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA. Author Bauchan, Gary R. Electron & Confocal Microscopy Unit, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, U. S. A. Author Barros-Battesti, Darci M. Departamento de Medicina Veterinária Preventiva e Saúde Animal, FMVZ-USP, São Paulo, SP, Brazil. & Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias-UNESP, Jaboticabal, SP, Brazil. text Acarologia 2018 2018-11-30 1938 4 976 986 http://dx.doi.org/10.24349/acarologia/20184304 journal article 10.24349/acarologia/20184304 2107-7207 5390982 DC1A4B29-31D8-498C-8F9F-63A12E4E7C13 Eutrombicula batatas ( Linnaeus, 1758 ) New records1 larva (IBSP 349D), Butantan, São Paulo municipality, São Paulo state , 09-VI-1935 , “rodent” unidentified ( Rodentia : Cricetidae ); 1 larva (IBSP 350A), same locality, 06-VI-1935 , “rodent” unidentified ( Rodentia : Cricetidae ); 2 larvae (IBSP 383), same locality, 09-X-1933 , Rattus norvegicus ( Rodentia : Muridae ); 5 larvae (IBSP 484C), same locality, 29-X-1933 , Didelphis aurita ( Didelphimorphia : Didelphidae ); 1 larva (IBSP 1690), same locality, Crotophaga ani ( Cuculiformes : Cuculidae ); 1 larva (IBSP 1647), Morro Grande, Cotia municipality, São Paulo state , 20-II-1939 , “owl” unidentified ( Strigiformes ; Strigidae ); 2 larvae (IBSP 1638), Wenceslau Braz municipality, Parana state , 03-III-1939 , Cavia aperea ( Rodentia : Caviidae ); 2 larvae (IBSP 2128), Santa Cruz do Sul municipality, Rio Grande do Sul state , 04-XII-1951 , Hidrochoerus hidrochoeris ( Rodentia : Caviidae ); 10 larvae (IBSP 13285), Tucuruí municipality, Pará state , no date, Nyctibius griseus ( Nyctibiiformes : Nyctibiidae ). Molecular analysis Two samples ( paratypes ) of the new species were submitted to PCR and were positive to the 18S gene PCR and amplified two identical fragments of 409-bp (GenBank accession numbers: MG707783 and MG707784 ). BLAST comparisons of these sequences showed 99.7% (408/409-bp) of identity with homologous sequences from Eutrombicula splendens ( KP325057 ) and 99.5% (407/409-bp) from Quadraseta brasiliensis ( MF113413 ; MF113412 ; KY934464 ). None of the samples amplified for the COI gene. In addition, we obtained partial sequences for the 18S gene from the new species. These sequences are the first molecular identification of this genus for Brazil . At this time, it is still not possible to infer molecular differences between species that have a high similarity to Eutrombicula daemoni n. sp. based on an analysis of their 18S sequences, due to the slow rate of evolution of this gene, so different genera when compared have high similarity, which reflects that these species belong to a same family of mites. Unfortunately, there are only these two species of Eutrombicula with 18S sequences deposited in Genbank. It is necessary to enrich the gene bank for species of this genus for future comparisons. Until now, we do not have recent material of E. batatas for molecular analysis and didn’t possible comparisons with the new species.