A new species of the genus Eutrombicula Ewing, 1938 (Trombidiformes: Trombiculidae) and new records for the species Eutrombicula batatas (Linnaeus, 1758) in Brazil
Author
Bassini-Silva, Ricardo
Departamento de Medicina Veterinária Preventiva e Saúde Animal, FMVZ-USP, São Paulo, SP, Brazil.
Author
Jacinavicius, Fernado de Castro
Departamento de Medicina Veterinária Preventiva e Saúde Animal, FMVZ-USP, São Paulo, SP, Brazil. & Laboratório Especial de Coleções Zoológicas, Instituto Butantan, São Paulo, SP; Brazil.
Author
Muñoz-Leal, Sebastián
Departamento de Medicina Veterinária Preventiva e Saúde Animal, FMVZ-USP, São Paulo, SP, Brazil.
Author
Maturano, Ralph
Departamento de Zoologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
Author
Welbourn, W. Calvin
Florida State Collection of Arthropods, Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, FL, USA.
Author
Ochoa, Ronald
Systematic Entomology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA.
Author
Bauchan, Gary R.
Electron & Confocal Microscopy Unit, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, U. S. A.
Author
Barros-Battesti, Darci M.
Departamento de Medicina Veterinária Preventiva e Saúde Animal, FMVZ-USP, São Paulo, SP, Brazil. & Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias-UNESP, Jaboticabal, SP, Brazil.
text
Acarologia
2018
2018-11-30
1938
4
976
986
http://dx.doi.org/10.24349/acarologia/20184304
journal article
10.24349/acarologia/20184304
2107-7207
5390982
DC1A4B29-31D8-498C-8F9F-63A12E4E7C13
Eutrombicula batatas
(
Linnaeus, 1758
)
New records
—
1 larva
(IBSP 349D), Butantan,
São Paulo
municipality,
São Paulo state
,
09-VI-1935
, “rodent” unidentified (
Rodentia
:
Cricetidae
);
1 larva
(IBSP 350A), same locality,
06-VI-1935
, “rodent” unidentified (
Rodentia
:
Cricetidae
);
2 larvae
(IBSP 383), same locality,
09-X-1933
,
Rattus norvegicus
(
Rodentia
:
Muridae
);
5 larvae
(IBSP 484C), same locality,
29-X-1933
,
Didelphis aurita
(
Didelphimorphia
:
Didelphidae
);
1 larva
(IBSP 1690), same locality,
Crotophaga ani
(
Cuculiformes
:
Cuculidae
);
1 larva
(IBSP 1647), Morro Grande,
Cotia municipality,
São Paulo state
,
20-II-1939
, “owl” unidentified (
Strigiformes
;
Strigidae
);
2 larvae
(IBSP 1638), Wenceslau Braz municipality,
Parana state
,
03-III-1939
,
Cavia aperea
(
Rodentia
:
Caviidae
);
2 larvae
(IBSP 2128), Santa Cruz do Sul municipality,
Rio Grande do Sul state
,
04-XII-1951
,
Hidrochoerus hidrochoeris
(
Rodentia
:
Caviidae
);
10 larvae
(IBSP 13285), Tucuruí municipality,
Pará state
, no date,
Nyctibius griseus
(
Nyctibiiformes
:
Nyctibiidae
).
Molecular analysis
Two samples (
paratypes
) of the new species were submitted to PCR and were positive to the 18S gene PCR and amplified two identical fragments of 409-bp (GenBank accession numbers:
MG707783
and
MG707784
). BLAST comparisons of these sequences showed
99.7% (408/409-bp) of identity with homologous sequences from
Eutrombicula splendens
(
KP325057
) and 99.5% (407/409-bp) from
Quadraseta brasiliensis
(
MF113413
;
MF113412
;
KY934464
). None of the samples amplified for the COI gene.
In addition, we obtained partial sequences for the 18S gene from the new species. These sequences are the first molecular identification of this genus for
Brazil
. At this time, it is still not possible to infer molecular differences between species that have a high similarity to
Eutrombicula daemoni
n. sp.
based on an analysis of their 18S sequences, due to the slow rate of evolution of this gene, so different genera when compared have high similarity, which reflects that these species belong to a same family of mites.
Unfortunately, there are only these two species of
Eutrombicula
with 18S sequences deposited in Genbank. It is necessary to enrich the gene bank for species of this genus for future comparisons. Until now, we do not have recent material of
E. batatas
for molecular analysis and didn’t possible comparisons with the new species.