Short-spored Subulicystidium (Trechisporales, Basidiomycota): high morphological diversity and only partly clear species boundaries Author Ordynets, Alexander https://orcid.org/0000-0002-2904-7094 Department of Ecology, FB 10 Mathematics and Natural Sciences, University of Kassel, Heinrich-Plett-Strasse 40, 34132 Kassel, Germany a.ordynets@uni-kassel.de Author Scherf, David Department of Ecology, FB 10 Mathematics and Natural Sciences, University of Kassel, Heinrich-Plett-Strasse 40, 34132 Kassel, Germany Author Pansegrau, Felix Department of Ecology, FB 10 Mathematics and Natural Sciences, University of Kassel, Heinrich-Plett-Strasse 40, 34132 Kassel, Germany Author Denecke, Jonathan Department of Ecology, FB 10 Mathematics and Natural Sciences, University of Kassel, Heinrich-Plett-Strasse 40, 34132 Kassel, Germany Author Lysenko, Ludmila Department of Ecology, FB 10 Mathematics and Natural Sciences, University of Kassel, Heinrich-Plett-Strasse 40, 34132 Kassel, Germany Author Larsson, Karl-Henrik Natural History Museum, University of Oslo, P. O. Box 1172 Blindern, 0318 Oslo, Norway Author Langer, Ewald Department of Ecology, FB 10 Mathematics and Natural Sciences, University of Kassel, Heinrich-Plett-Strasse 40, 34132 Kassel, Germany text MycoKeys 2018 2018-06-27 35 41 99 http://dx.doi.org/10.3897/mycokeys.35.25678 journal article http://dx.doi.org/10.3897/mycokeys.35.25678 1314-4049-35-41 FF86652FFFAF2557FFB20B15FFF6330B 1305473 Subulicystidium fusisporum Ordynets & K.H.Larss. sp. nov. Figs 4a-c ; 10e Diagnosis. Differs from Subulicystidium longisporum (Pat.) Parmasto by fusiform basidiospores which are ca. 10-13 µm long and 2.5-3.5 µm broad. Type . PUERTO RICO . Municipio Rio Grande , Luquillo Mts , El Verde Research Area , between Field Station and 16-hectare grid, 320-380 m , 18.3233 , -65.8172 , on fallen tree log, 9 Jun 1998 , K.-H.Larsson ( KHL 10360 in GB) . Etymology. fusisporum (Lat.), having fusiform basidiospores. Description. Basidiomata annual, effused, resupinate, soft and fragile, arachnoid, thin, loosely adnate. Hymenophore smooth, finely velutinous due to numerous protruding cystidia, whitish. Margin thinning out, adnate. system monomitic. All septa with clamps. Subiculum thin, with loosely interwoven richly branched hyphae 2.5-3.5 µm wide, usually thin-walled, hyaline and smooth. Subhymenium thin, with hyphae slightly broader than in subiculum, 2.7-4 µm wide, compactly arranged, often slightly thick-walled and covered with hyaline crystalline sheath. Cystidia subulate, 65-90 x 3.5-5 µm including encrustation, projecting up to 40 µm , without or occasionally with basal swelling (up to 6 µm wide), terminal, with thick hyaline cell wall and outer hyaline crystalline sheath covering the whole cystidium except the thin-walled, acuminate apex. Crystal protrusions on cystidium are small to moderately large and clearly rectangular and arranged in longitudinal rows. Basidia suburniform, 12-14 x 4.5-6 µm , thin-walled, with 4 sterigmata and a basal clamp, often with a hyaline crystal collar at the base. Basidiospores fusiform, L=(9.7-)10.7-12.8(-13.3) µm , W=(2.1-)2.4-3.4(-3.7) µm , Q= (3.0-)3.3-4.9(-5.9), N=127/3, with minute apiculus, smooth, thin-walled, hyaline, occasionally with oil drops, negative in Melzer's reagent. Tolerance limits for basidiospore length, width and length to width ratio in S. fusisporum based on 3 sequenced specimens are provided in the Table 2 . Table 2. 90% tolerance limits defined for the 90% probability level for the mean basidiospore length, width and length to width ratio for Subulicystidium species with at least 3 sequenced specimens. The following specimens were used to estimate tolerance limits for species: Subulicystidium fusisporum : GB:KHL 9093, 10360 and 12761; S. harpagum : GB:KHL 10733, O:F:LR 15736, KAS:L 0244 and 1726a; S. parvisporum : KAS:GEL 5032, KAS:L 0140 and 1226 and LY 12750; S. robustius : GB:KHL 10039, 10272, 10780 and 10813.
Measurement type Estimate Species
Subulicystidium fusisporum Subulicystidium harpagum Subulicystidium parvisporum Subulicystidium robustius
Spore length, µm Sample mean 11.78 6.74 5.61 9.78
Lower limit of 90% tolerance interval 9.64 4.34 4.78 7.81
Upper limit of 90% tolerance interval 13.92 9.13 6.43 11.75
Spore width, µm Sample mean 2.92 2.6 2.51 3.00
Lower limit of 90% tolerance interval 1.65 1.62 2.06 2.44
Upper limit of 90% tolerance interval 4.19 3.58 2.95 3.57
Spore length/width ratio Sample mean 4.09 2.63 2.25 3.27
Lower limit of 90% tolerance interval 1.89 0.83 1.91 2.52
Upper limit of 90% tolerance interval 6.28 4.42 2.59 4.02
Additional specimens examined. COSTA RICA . Puntarenas : Coto Brus , Sabalito , Zona Protectora Las Tablas , La Neblina , 8.9149 , -82.7719 , on stem of angiosperm tree, 5 Nov 2004 , K.-H.Larsson ( KHL 12761 in GB) . COTE D'IVOIRE . Abidjan : Foret du Banco , 5.3932 , -4.0525 , on dead wood, 6 Jul 1974 , G.Gilles (LY 7375) . JAMAICA . Cornwall County : Trelawny parish , N of Crowlands , trail/road into park area, 18.2611 , -77.6511 , on stem of angiosperm tree, 10 Jun 1999 , K.-H.Larsson ( KHL 10612 in GB) . PUERTO RICO . Municipio Rio Grande , Luquillo Mts , El Verde Research Area , between Field Station and 16-hectare grid, 320-380 m , 18.3233 , -65.8172 , on strongly decayed stem of angiosperm tree, 19 Jun 1996 , K.-H.Larsson (KHL 9093 in GB), on uprooted angiosperm tree, 19 Jun 1996 , K.-H.Larsson (KHL 9061 in GB) . Remarks on species. Amongst the species considered in this study, S. fusisporum is the most probable to be confused with S. longisporum . However, careful measurement of basidiospores (length below 13 µm , see Fig. 10e vs. 10g) and rDNA sequence identity clearly point to the species of its own. The regular rectangular shape of crystal protrusion as well as their dense arrangement in longitudinal rows on cystidia in S. fusisporum is also prominent.