Glyphidrilus wararamensis Chanabun & Panha, 2013

Chanabun, Ratmanee, Sutcharit, Chirasak, Tongkerd, Piyoros & Panha, Somsak, 2013, The semi-aquatic freshwater earthworms of the genus Glyphidrilus Horst, 1889 from Thailand (Oligochaeta, Almidae) with re-descriptions of several species, ZooKeys 265, pp. 1-76 : 48-51

33. Glyphidrilus wararamensis Chanabun & Panha sp. n. Figs 31 32 38 Table 1

Type material.

Holotype: CUMZ 3257 ( Fig. 32 ), in muddy swamp at stream near Wattham Wararam, Phanom, Suratthani, Thailand, 08°53'13.6"N, 098°39'54.5"E, 3 m amsl, 17 February 2009. Paratypes: 4 adults and 53 juveniles (CUMZ 3258), 3 adults (ZMH 145670), and 3 adults (NHM), same collection data as holotype.

Other material examined.

3 adults and 3 juveniles (CUMZ 3259), Khaosok National Park, Phanom, Suratthani, Thailand, 08°56'16.14"N, 098°31'48.56"E, 286 m amsl, 17 February 2009. 32 adults and 8 juveniles (CUMZ 3260), Klongsok, Phanom, Suratthani, Thailand, 08°52'45.8"N, 098°33'10.4"E, 61 m amsl, 6 October 2011. 6 adults and 38 juveniles (CUMZ 3261), Namlod cave, Phanom, Suratthani, Thailand, 08°46'57.7"N, 098°50'57.1"E, 6 m amsl, 11 December 2010.

Etymology.

The name " wararamensis " is given to this species for its type locality at the stream near Wattham Wararam, Phanom, Suratthani, Thailand. The locality is a part of this famous stream and is the first record of this genus at this locality.

Diagnosis.

The unique characters of Glyphidrilus wararamensis sp. n. are distinct expanded tissues of wi in 20, 21-26, 27, with clitellum in 11, 12, 13-33, 34, 35; gm: lateral paired or asymmetrical on bc in 14, 15, 17, 18-19, 20, 27, median unpaired on aa in 11-13, 14, 15, 17, 18-19, 20, 28, 29-30; he in 8-11; sv in 9-12; two pairs of ov in 13-14; testis in 10-11; sc sessile, elongated oval or globular in 13/14-17/18, three to nine pairs on each side per segment ( Table 1 ).

Description of Holotype.

Dimensions: body length 112 mm, diameter 3.3 mm in segment 8, 3.1 mm before clitellar wi in segment 19, 3.1 mm behind wi in segment 27 in intraclitellar region. Body cylindrical in anterior part, quadrangular in transverse section behind clitellum. 335 segments. Body colour pale brown with variations from red to pink at adjacent tissues of wi portion in different individuals of newly collected specimens after placement in 30% ethanol for narcotization. Dorsal surface considerably broader than the ventral at posterior end. Clitellar wi on ventro-lateral part of clitellum in 20-26, 3.0 mm in height, and about 2.2 mm in width on both sides. Prostomium zygolobous. Dorsal pores absent. Clitellum annular in 13-35. Four pairs of setae per segment from 2, setal formula aa:ab:bc:cd:dd = 0.6:0.2:0.8:0.3:0.7 in segment 8 and 0.9:0.5:1.4:0.6:1.2 in postclitellar segments. Female pores, male pores, and spermathecal pores are not visible. Gm: lateral paired or asymmetrical on bc in 17-19, 27, median unpaired on aa in 11-13, 15, 18-19, 29-30.

Septa 5/6-8/9 thicker, 9/10-14/15 thick and 15/16 to the last segment thin. Gi small globular within 6-8. Intestine enlarged from 14. Dorsal blood vessel aborted anterior to 8. He in 8-11. No distinguishable np in first eleven segments. Sv in 9-12. Ov in 13-14. Testis in 10-11. Prostate and accessory glands absent. Sc sessile, elongated oval or globular in 13/14-17/18, 0.1-0.3 mm in diameter, three to nine on each side per segment.

Variation.

Body length of adult paratypes (10) and non-types (41) ranged from 18+-120 mm (48.6 ± 21.2), with 46+-279 segments. Wi in 20, 21-26, 27, with clitellum in 11, 12, 13-33, 34, 35; gm: lateral paired or asymmetrical on bc in 14, 15, 17-19, 20, 27, median unpaired on aa in 11-13, 14, 15, 17, 18-19, 20, and 28, 29-30.

Distribution.

The new species is known from the worm cast covered top soil at several locations of Suratthani, South Thailand, found on the shore connected to water.

Remarks.

Glyphidrilus wararamensis sp. n. similar to Glyphidrilus tuberosus reported from Kenduapatna Canal, Ponds at Pubhans, Cuttack, Mud at edge of River Tista, Jalpaiguri in the locations of wi however, Glyphidrilus tuberosus has shorter clitellum in 14, 15, 16-28, 29, 30 and sc in 14-15.The new species similar to Glyphidrilus singaporensis from Bukit Timah, Singapore in the locations of wi, whereas Glyphidrilus singaporensis has shorter clitellum in 18-27, ½28, 29, 30, 31, three pairs of he in 9-11 and sc in 14-17 or 13/14-16/17.

The new species differs from Glyphidrilus malayanus from Malay Peninsula which has a shorter wi in ¾18, 18-21, ½22 and shorter clitellum in 15, 16, 17-23, 24, 25, three pairs of he in 9-11 and sc in 14/15-16/17.It differs from Glyphidrilus birmanicus from Burma by Glyphidrilus birmanicus has longer wi in 21, 22, 23-28, 29, 30, longer clitellum in 12, 13-43, 44 and he in 7-11.Differs from Glyphidrilus gatesi from Johor, Malaysia by Glyphidrilus gatesi has different locations of wi in 18, ½19, 19- ½24, 24, with shorter clitellum in ½17, 17, 18-25, ½26, 26, he in 9-11 and sc in 15-17. Differs from Glyphidrilus vangviengensis from Laos by Glyphidrilus vangviengensis has longer wi in 24, 25-31, 32, with shorter clitellum in 19, 20-35, 36, 37, 38, he in 7-11 and lacks sc.

The new species differs from Glyphidrilus trangensis sp. n. has shorter wi in 22, 23-27, 28, with shorter clitellum in 17, 18-30 and sc in 18-21. Differs from Glyphidrilus kratuensis sp. n. by Glyphidrilus kratuensis sp. n. has wi in 23, 24-28, 29, 30, shorter clitellum in 18-30, 31, 32 and sc in 14/15-17/18 ( Table 1 ).

Figures

Figure 31. Photographs showing the A type locality of Glyphidrilus wararamensis sp. n. in a muddy swamp at a stream near Wat Tham Wararam, Phanom, Suratthani, B Glyphidrilus wararamensis sp. n. casts and C coloration of newly collected paratype CUMZ 3258 just after the first preservation step in 30 % (v / v) ethanol.

Figure 32. Morphology of the holotype (CUMZ 3257) of Glyphidrilus wararamensis sp. n., showing the A external ventral and B internal dorsal views.

Figure 38. Distribution map of the new Glyphidrilus species from Thailand. Filled hexagon: Glyphidrilus vesper sp. n., Open trapezoid: Glyphidrilus wararamensis sp. n., Filled trapezoid: Glyphidrilus trangensis sp. n., Filled star: Glyphidrilus kratuensis sp. n.