Biolus brunoi, Lumen, Ryan & Kamdńskd, Marcdn J., 2024

Lumen, Ryan & Kamdńskd, Marcdn J., 2024, evdsdon of the darkldng beetle genus Eurynotus (Blaptdnae: Platynotdnd % and new records of ovovdvdpary dn ºenebrdonddae, Zoological Journal of the Linnean Society 201 (201), pp. 358-386 : 376-377

3. Biolus brunoi sp.nov.

( Figs 1B , 2C , 6J , 7C , 8C )

urn:lsid:zoobank.org:act:57A85549-53E4-4EA1-ADF2- DDE094DD4D91.

Diagnosis: This taxon is most similar to some specimens of Biolus asperipennis . In addition to characteristics provided above, B. brunoi can be separated from other Biolus species via: tarsal setae dark/black rather than golden; tubercles absent on elytral disc; abdominal ventrite V with large setigerous punctures; head

punctation coarse and closely spaced (separated by ≤1 puncture diameter). Additionally, B. brunoi aedeagal parameres are dis - tinctly pointed (truncate or apically blunted or rounded in all other species of Biolus and Eurynotus ).

Description: Length 15–18 mm; width 7.5–10 mm. Head: Coarsely punctate, punctures closely spaced, separated by ≤1 puncture diameter. Mentum without apical notch. Eye with impressed sulcus situated around perimeter of dorsal lobe. Prothorax: Pronotum coarsely punctate, punctures separated by ≤1 puncture diameter. Hypomeron shallowly and coarsely punctate and lightly sculptured/wrinkled. Pterothorax: Elytron not costate, without tubercles on disc. Elytral interval X terminates between hind coxae and elytra base. Epipleuron narrower than width of elytral intervals 9 + 10. Elytral inter - vals not obscured by epipleuron in ventral view. Abdomen: Punctate. Abdominal ventrite V coarsely punctate, punctures separated by ~1 puncture diameter with larger, setigerous punctures intermixed. Females without median apical notch. Legs: Male hind tibiae very slightly flattened/twisted, ap - pearing bent. Female legs unmodified. Tarsi with black or dark setae. Males: pro/meso tarsi enlarged, with ventral, tomen - tose pads. Male terminalia: Parameres strongly tapered and pointed apically. Female terminalia: Bursa copulatrix sac -like and without sclerites, spermatheca thick and coiled, accessory pouch absent.

Material examined: Holotype (TMNH): S. Afr.; E Cape; 1070m 30km NE Somerset East 32.31 S — 25.34 E / 30.11.2007; E -Y:3767 general collecting leg. Ruth Müller. Paratypes (N = 17): TMNH: ‘ S. Afr., Cape - Karroo Woodcliff farm 31.57 S - 24.38 E; 13.9.1983; E -Y:2004 singled day leg. Penrith (2 specimens)’, ‘ S.Afr.; E Cape 1450m 50km NE Graaf Reinet 32.09 S - 24.51 E; 27.11.2007.E -Y: 3766 under stones leg. Ruth Müller (5 specimens)’, ‘ S. Afr.; E Cape; 1070m 30km NE Somerset East 32.31 S - 25.34 E / 30.11.2007; E -Y:3767 general collecting leg. Ruth Müller (4 specimens)’, ‘ S.Afr., E. Cape Prov. Swaershoek Pass 32.17 S - 25.32 E, 10.10.1984; E -Y: 2142 ground and vegetation leg. R. Müller’, ‘ S.Afr.; E Cape; 1070m 30km N Bedford 32.24 S - 26.05 E, 12.12.2007; E -Y 3768 general collecting leg. Ruth Müller (3 specimens)’. MNHN: ‘ Cap. B. Esp. ’.

Etymology: Named after Bruno, dear friend of Ruth Müller (Pretoria, South Africa).

Distribution: South Africa (Fig. 9).

Figures

Figure 2. Biolus stat.nov.species.A, Biolus analisetosus (Koch) comb. nov. Holotype; B, B.asperipennis (Mulsant and Rey) stat.res., comb.nov. Lectotype; C, B. brunoi sp. nov. Holotype; D, B. denticosta (Mulsant and Rey) comb. nov. Lectotype; E, B. graafi (Koch) comb. nov. Holotype; F,B. granulatus (Fabricius) comb. nov. Lectotype; G, B. omeri (Koch) comb.nov.Holotype; H, B. sauroides (Koch) comb. nov. Holotype; I, Eurynotus norrisi Mulsant and Rey syn. nov. of B. granulatus, Lectotype.

Figure 6. Characters used in the cladistic analysis.Arrows indicate particular states of given characters from the morphological matrix [e.g.

Figure 7. Phylogeny of morphology, ovovivipary, and female reproductive structures of Eurynotina.A, Eurynotus barbosai, 1st instar larva dissected from bursa copulatrix. B, Biolus omeri, genital tubes. C, B. brunoi, spermatheca and ovipositor.D, E. capensis, genital tubes of two individuals.E, B.analisetosus, sclerite and egg dissected from bursa copulatrix. F, B. granulatus, sclerite from bursa copulatrix.G, E. asperatus, paired sclerites on distal part of bursa copulatrix. H, Oncotus cedrimontis, bursa copulatrix. Abbreviations: c1–c4, lobes of coxities. Numbers in parentheses represent characters and states used in analysis.

Figure 8. Aedeagal morphology of Eurynotina species.A, Biolus analisetosus; B, B.asperipennis; C, B. brunoi sp. nov.; D, B. denticosta; E, B. graafi; F, B. granulatus; G, B. omeri; H, B. sauroides; I, Eurynotus asperatus; J, E.barbosai; K, E. capensis; L, E. graniderma; M, Capidium tuberculatum; N, Oncotus cedrimontis; O, Heteropsectropus amaroides; P, Schyzoschelus africanus. Parameres highlighted red. Scale = 1mm.

Figure1. Ovoviviparyin Tenebrionidae and its geographical distribution.A, sympatricPlatynotini species displaying different reproductionstrategies: Biolusgranulatus–ovoviviparous,Crypticanus sp.–oviparous.B, female genital tract ofBiolus brunoi sp.nov.with developed 1stinstar larva.Scale