Campiglossa ialong David, Salini & Hancock, 2020

David, Karamankodu Jacob, Hancock, David Lawrence, Salini, Santhamma, Gracy, Ramasamy Gandhi & Sachin, Kandiyil, 2020, Taxonomic notes on the genus Campiglossa Rondani (Diptera, Tephritidae, Tephritinae, Tephritini) in India, with description of three new species, ZooKeys 977, pp. 75-100 : 75

Campiglossa ialong David, Salini & Hancock sp. nov. Figures 1 , 2-9 , 10-11

Diagnosis.

Medium-sized fly (3.74-4.25 mm), body grey, pollinose, with white setulae; scutum without prominent stripes; abdomen with submedian black markings; wing with reticulate pattern.

Description.

Male (body length, 3.74-4.25 mm; wing length, 3.76-4.04 mm).

Head: Slightly higher than long (head ratio 0.83-0.86); frons fulvous (frons-head ratio 0.38-0.40), with a medial band of pruinosity from ocellar triangle to lunule leaving two dark fuscous lateral bands devoid of pruinosity; two frontal setae (three in a few specimens); two subequal orbital setae (posterior one white); well-developed proclinate ocellar seta (0.7 length of medial vertical seta); lateral vertical seta white; medial vertical seta black; paravertical seta white; postocular setae intermixed black and white. Scape, pedicel, and flagellomere concolorous with frons; pedicel plus flagellomere shorter than face; arista bare; face concave with raised epistomal margin; gena and occiput fulvous. Eye ratio 0.64-0.69; gena-eye ratio 0.13-0.18; antenna-head ratio 0.45-0.47; arista-antenna ratio 1.20-1.45.

Thorax: Scutum grey pollinose, with three faint stripes and well-developed chaetotaxy (all setae black); one postpronotal lobe seta, one presutural supra-alar seta, one anterior notopleural seta, one posterior notopleural seta, one dorsocentral seta near transverse suture, placed anterior of postsutural supra-alar seta and posterior notopleural seta, one presutural supra-alar seta, one postalar seta, one intra-alar seta, one prescutellar acrostichal seta. Anepisterum grey with single black anepisternal seta in line with posterior notopleural seta; anepisternum covered with tiny white setulae; thick white setulae posteriorly near phragma; anepimeron without any black setae, with thick stubby white setulae anteriorly; katepisternum with single black seta posterior to phragma in dorsal region; anatergite and katatergite grey without any setulae; haltere pale yellow. Scutellum flat, yellow with sparse white setulae; two scutellar setae; apical scutellar seta 2/3 length of basal scutellar seta. Mediotergite grey, without setulae.

Legs: All segments unicolorous, yellowish orange; fore femur with single row of five or six stout ventral setae, two rows of dorsal setae; mid and hind femur covered with tiny black setulae. Mid tibia with four apical spines, one elongate, the others all 1/4 length of prominent spine.

Wing: Reticulate pattern, with hyaline and yellow spots; basal 1/3 hyaline with faint brown markings; apical 2/3 dark brown with numerous hyaline and yellow spots. Cell bc hyaline; cell c hyaline with two faint brown markings; pterostigma dark brown with a medial, yellow spot/patch; apex of cell r1 and r2+3 black without any hyaline spots; cell r2+3 with a preapical dumbbell-shaped spot. Cell r1 with three broad hyaline patches and irregular yellow spots or patches; cell r2+3 hyaline only in basal portion, rest brown to black with irregular yellow spots and, broad hyaline markings that are extensions of the hyaline markings from cell r1 and preapical dumbbell-shaped spot (separate spots in a few specimens). Cell br predominantly hyaline, with irregular brown markings; cell r4+5 predominantly black or brown with an apical hyaline spot, two preapical spots, numerous yellow spots, and a broad basal hyaline spot. Cells bm and bcu hyaline; basal 2/3 of cell dm largely hyaline, with narrow basal and submedial brown transverse bands, apical 1/3 brown with hyaline spots; cell m with four broad irregular markings; cell cu2 and anal lobe predominantly hyaline with irregular brown markings.

Abdomen: Grey pollinose, with white setulae; tergites 1+2 broad, with reduced pruinosity; tergites 3-5 with broad, submedian, quadrate patches; tergite 5 is 2-2.25 × broader than tergites 3 and 4, with apical black setae. Sternites grey; posterior margin of sternite 5 with shallow concavity.

Male genitalia: Epandrium elongate, tapering towards surstylar end (lateral view) without clear demarcation between surstylus and epandrium. Lateral surstylar flange as high as epandrium, serrate throughout its entire length; apex of lateral surstylus without clear demarcation of anterior and posterior lobes; proctiger hyaline, microtrichose. Epandrium oval in outline (caudal view); medial surstylus well developed with prensisetae. Phallus elongate (1.34 mm); preglans area strongly spinulose; basal lobe absent; glans of phallus sclerotised, 1/2 length of phallus (0.78 mm), with well-developed, elongate, tubular acrophallus.

Female: Similar to male except larger (body length 4.56-5.23 mm; wing length 4.14-4.62 mm). Oviscape shining black (1.66 mm); taeniae short (0.25 of total length of eversible membrane); spicules on anterior end of eversible membrane (1.30 mm) conical with pointed apex, whereas spicules of distal end conical with blunt apex. Aculeus elongate (1.38 mm) with pointed tip, devoid of preapical indentations. Spermatheca dark brown, oval, with transverse striations.

Type material.

Holotype ♂, INDIA: Meghalaya, Mihmyntdu, Ialong, 25.476°N, 92.226°E, 13.x.2019, Salini S. Paratypes: 21♂♂, 7♀♀, same data as above except for two males with collector's name David K.J. 1 larva on slide (III instar), same data as above (NBAIR).

DNA barcode.

GenBank accession number MT169786 (1♂, INDIA: Meghalaya, Mihmyntdu, Ialong, 25.476°N, 92.226°E, 24.x.2019, K.J. David).

Etymology.

The specific epithet is a noun in apposition and refers to the type locality.

Third instar larva (Figs 12-14 ).

Larva short, stout (3.22-3.51 mm), whitish to dull white. Mouthhook pointed with a well-developed preapical tooth as long as apical mouthhook; ventral apodeme 2 × broader than mouthhook; mandibular neck not prominent; dorsal apodeme pointed dorsally; labial sclerite elongate; pharyngeal sclerite 2.5 × longer than broad; hypopharyngeal bridge reduced; parastomal bar prominent; dorsal bridge pointed anteriorly; ventral bridge of hypopharyngeal sclerite pointed anteriorly; anterior sclerite not well developed; dorsal cornua undivided; ventral cornua with two branches. Anterior spiracle weakly sclerotised, with six tubules. Posterior spiracle with spiracular slits oval, slightly longer than wide, devoid of transverse striations; spiracles separated by distance equal to the length of each slit; dorsal and ventral spiracular bundle with 2-6 single hairs; lateral spiracular bundle with 4-6 single hairs.

Remarks.

Campiglossa ialong is most similar to C. iracunda (Hering) in appearance but with only one hyaline spot at the apex of cell R2+3, as in C. siamensis ( Hardy 1973). However, the black posterior notopleural seta differs from C. siamensis , which has a brown or yellowish seta. As per the phylogenetic tree (Fig. 51 ), it is paraphyletic with the misella group.

Figures

Figure 1. Habitus (male) of Campiglossa ialong David, Salini & Hancock, sp. nov.

Figure 51. Maximum likelihood phylogram of 17 Campiglossa and one Tephritis (outgroup) DNA barcode sequences using General Time Reversible model. The number at each node is the boostsrap value based on ML analysis.

Figures 2 - 9. Campiglossa ialong David, Salini & Hancock, sp. nov. 2 head 3 thorax (dorsal view) 4 thorax (lateral view) and legs 5 abdomen 6 wing 7 epandrium and surstyli (lateral view) 8 epandrium and surstyli (posterior view) 9 glans of phallus.

Figures 10, 11. Campiglossa ialong David, Salini & Hancock, sp. nov. 10 spermatheca 11 ovipositor 11 a spicules on proximal end of eversible membrane 11 b spicules on distal end of eversible membrane 11 c aculeus tip.

Figures 12 - 14. Larval morphology of Campiglossa ialong David, Salini & Hancock, sp. nov. 12 cephalopharyngeal skeleton 13 anterior spiracle 14 posterior spiracles.