Polypedates mutus (Smith, 1940) Burmese

Mulcahy, Daniel G., Lee, Justin L., Miller, Aryeh H., Chand, Mia, Thura, Myint Kyaw & Zug, George R., 2018, Filling the BINs of life: Report of an amphibian and reptile survey of the Tanintharyi (Tenasserim) Region of Myanmar, with DNA barcode data, ZooKeys 757, pp. 85-152 : 85

Polypedates mutus (Smith, 1940) Burmese

Description.

All individuals are immature; six females 59.9-66.6 mm, three males 45.3-50.3, and a sex indeterminate specimen 45.2 mm SVL (n=10).

Natural history notes.

See preceding species account.

General Distribution.

Tanintharyi Myanmar, Thailand, Yunnan and Guangxi, China.

Molecular Data.

The second clade of our Polypedates , and an additional specimen from Mandalay (USNM 587059) were placed in a COIBIN with four individuals from Thailand identified as Polypedates sp. Our specimens were placed in a 16S clade with individuals identified as P. impresus ( Pan et al. 2013) and Polypedates cf. mutus 2 of Kuraishi et al. (2012). Note, older specimens in GenBank in this clade are labeled as P. leucomystax , P. megacephalus , and P. sp. The very recently published paper examining Thailand species of Polypedates with COI data (Buddhachat and Suwannpoom 2018) identified five major clades in the P. leucomystax complex. Our specimens were placed in their "Northern A Polypedates sp." clade (not shown). Our ongoing work (Wilkinson, Mulcahy, Zug, in prep.) suggests that this clade represents P. mutus senus stricto (the P. mutus 1 clade of Kuraishi et al. 2012).

Specimens examined.

USNM 587009-018, USNM 587059.

Red List status.

LC.

Additional rhacophorids.

We sequenced three individuals initially identified as Polypedates teraiensis from Sagaing (USNM 524030), Yangon (USNM 587048), and Bago (587049). These were all placed in their own COIBIN and in a 16S clade with specimens from GenBank identified as P. teraiensis (AB530512-21) and two individuals (AB728167-8) labeled P. leucomystax , presumably misidentified. Additionally, we sequenced three additional specimens initially identified as Chiromantis spp. Two specimens (USNM 560923, USNM 560927) from Mandalay initially identified as C. hansenae , were placed in their own COIBIN and in a 16S clade with other individuals from GenBank identified as Chiromantis doriae . There is considerable genetic variation among the C. doriae specimens in GenBank, indicating that C. doriae as currently used is a species complex in need of revision. The third specimen (USNM 524023) from Sagaing was initially identified as C. nongkhorensis , but was placed at the base of the 16S clade containing C. nongkhorensis and C. doriae specimens from GenBank ( Aowphol et al. 2013). This specimen may represent a new species; however, we treat it as Chiromantis sp. A for now.